The hidden microbial ecosystem in the perennial ice from a Pyrenean ice cave

Over the last years, perennial ice deposits located within caves have awakened interest as places to study microbial communities since they represent unique cryospheric archives of climate change. Since the beginning of the twentieth century, the temperature has gradually increased, and it is estima...

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Autores: Ruiz-Blas, Fátima, Muñoz-Hisado, Víctor, García-López, Eva, Moreno Caballud, Ana, Bartolomé, Miguel, Leunda Esnaola, María, Martinez-Alonso, Emma, Alcázar, Alberto, Cid, Cristina
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/345373
Acceso en línea:http://hdl.handle.net/10261/345373
Access Level:acceso abierto
Palabra clave:Ice cave
Pyrenees
Global warming
Microbial community profiling
Next-generation sequencing
Environmental variables
Proteomics
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oai_identifier_str oai:digital.csic.es:10261/345373
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv The hidden microbial ecosystem in the perennial ice from a Pyrenean ice cave
title The hidden microbial ecosystem in the perennial ice from a Pyrenean ice cave
spellingShingle The hidden microbial ecosystem in the perennial ice from a Pyrenean ice cave
Ruiz-Blas, Fátima
Ice cave
Pyrenees
Global warming
Microbial community profiling
Next-generation sequencing
Environmental variables
Proteomics
title_short The hidden microbial ecosystem in the perennial ice from a Pyrenean ice cave
title_full The hidden microbial ecosystem in the perennial ice from a Pyrenean ice cave
title_fullStr The hidden microbial ecosystem in the perennial ice from a Pyrenean ice cave
title_full_unstemmed The hidden microbial ecosystem in the perennial ice from a Pyrenean ice cave
title_sort The hidden microbial ecosystem in the perennial ice from a Pyrenean ice cave
dc.creator.none.fl_str_mv Ruiz-Blas, Fátima
Muñoz-Hisado, Víctor
García-López, Eva
Moreno Caballud, Ana
Bartolomé, Miguel
Leunda Esnaola, María
Martinez-Alonso, Emma
Alcázar, Alberto
Cid, Cristina
author Ruiz-Blas, Fátima
author_facet Ruiz-Blas, Fátima
Muñoz-Hisado, Víctor
García-López, Eva
Moreno Caballud, Ana
Bartolomé, Miguel
Leunda Esnaola, María
Martinez-Alonso, Emma
Alcázar, Alberto
Cid, Cristina
author_role author
author2 Muñoz-Hisado, Víctor
García-López, Eva
Moreno Caballud, Ana
Bartolomé, Miguel
Leunda Esnaola, María
Martinez-Alonso, Emma
Alcázar, Alberto
Cid, Cristina
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
Ministerio para la Transición Ecológica y el Reto Demográfico (España)
Organismo Autónomo Parques Nacionales (España)
Ministerio de Economía y Competitividad (España)
Moreno Caballud, Ana [0000-0001-7357-584X]
Bartolomé, Miguel [0000-0002-2924-6261]
Cid, Cristina [0000-0001-5128-4558]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Ice cave
Pyrenees
Global warming
Microbial community profiling
Next-generation sequencing
Environmental variables
Proteomics
topic Ice cave
Pyrenees
Global warming
Microbial community profiling
Next-generation sequencing
Environmental variables
Proteomics
description Over the last years, perennial ice deposits located within caves have awakened interest as places to study microbial communities since they represent unique cryospheric archives of climate change. Since the beginning of the twentieth century, the temperature has gradually increased, and it is estimated that by the end of this century the increase in average temperature could be around 4.0°C. In this context of global warming the ice deposits of the Pyrenean caves are undergoing a significant regression. Among this type of caves, that on the Cotiella Massif in the Southern Pyrenees is one of the southernmost studied in Europe. These types of caves house microbial communities which have so far been barely explored, and therefore their study is necessary. In this work, the microbial communities of the Pyrenean ice cave A294 were identified using metabarcoding techniques. In addition, research work was carried out to analyze how the age and composition of the ice affect the composition of the bacterial and microeukaryotic populations. Finally, the in vivo effect of climate change on the cellular machinery that allow microorganisms to live with increasing temperatures has been studied using proteomic techniques.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
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info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/345373
url http://hdl.handle.net/10261/345373
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104205GB-C22
info:eu-repo/grantAgreement/AEI//MDM-2017-0737
info:eu-repo/grantAgreement/AEI//PTA2016-12325-I
info:eu-repo/grantAgreement/AEI//FJCI2017-31725
Ruiz-Blas, Fátima ; Muñoz-Hisado, Víctor ; García-López, Eva ; Moreno Caballud, Ana ; Bartolomé, Miguel ; Leunda, Maria ; Martinez-Alonso, Emma ; Alcázar, Alberto ; Cid, Cristina; 2023; "Data_Sheet_1_The hidden microbial ecosystem in the perennial ice from a Pyrenean ice cave.pdf. [Dataset]"; Figshare; version 1; https://doi.org/10.3389/fmicb.2023.1110091.s001
Ruiz-Blas, Fátima ; Muñoz-Hisado, Víctor ; García-López, Eva ; Moreno Caballud, Ana ; Bartolomé, Miguel ; Leunda, Maria ; Martinez-Alonso, Emma ; Alcázar, Alberto ; Cid, Cristina; 2023; "Table_2_The hidden microbial ecosystem in the perennial ice from a Pyrenean ice cave.XLSX [Dataset]"; Figshare; version 1; https://doi.org/10.3389/fmicb.2023.1110091.s002
Ruiz-Blas, Fátima ; Muñoz-Hisado, Víctor ; García-López, Eva ; Moreno Caballud, Ana ; Bartolomé, Miguel ; Leunda, Maria ; Martinez-Alonso, Emma ; Alcázar, Alberto ; Cid, Cristina; 2023; "Table_3_The hidden microbial ecosystem in the perennial ice from a Pyrenean ice cave.XLSX [Dataset]"; Figshare; version 1; https://doi.org/10.3389/fmicb.2023.1110091.s003
Ruiz-Blas, Fátima ; Muñoz-Hisado, Víctor ; García-López, Eva ; Moreno Caballud, Ana ; Bartolomé, Miguel ; Leunda, Maria ; Martinez-Alonso, Emma ; Alcázar, Alberto ; Cid, Cristina; 2023; "Table_4_The hidden microbial ecosystem in the perennial ice from a Pyrenean ice cave.XLSX [Dataset]"; Figshare; version 1; https://doi.org/10.3389/fmicb.2023.1110091.s004
https://doi.org/10.3389/fmicb.2023.1110091

dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
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spelling The hidden microbial ecosystem in the perennial ice from a Pyrenean ice caveRuiz-Blas, FátimaMuñoz-Hisado, VíctorGarcía-López, EvaMoreno Caballud, AnaBartolomé, MiguelLeunda Esnaola, MaríaMartinez-Alonso, EmmaAlcázar, AlbertoCid, CristinaIce cavePyreneesGlobal warmingMicrobial community profilingNext-generation sequencingEnvironmental variablesProteomicsOver the last years, perennial ice deposits located within caves have awakened interest as places to study microbial communities since they represent unique cryospheric archives of climate change. Since the beginning of the twentieth century, the temperature has gradually increased, and it is estimated that by the end of this century the increase in average temperature could be around 4.0°C. In this context of global warming the ice deposits of the Pyrenean caves are undergoing a significant regression. Among this type of caves, that on the Cotiella Massif in the Southern Pyrenees is one of the southernmost studied in Europe. These types of caves house microbial communities which have so far been barely explored, and therefore their study is necessary. In this work, the microbial communities of the Pyrenean ice cave A294 were identified using metabarcoding techniques. In addition, research work was carried out to analyze how the age and composition of the ice affect the composition of the bacterial and microeukaryotic populations. Finally, the in vivo effect of climate change on the cellular machinery that allow microorganisms to live with increasing temperatures has been studied using proteomic techniques.This research was funded by the grants PID2019-104205GBC22 and MDM-2017-0737 Unidad de Excelencia “Maria de Maeztu” - Centro de Astrobiología (INTA-CSIC), the Spanish Ministry of Science and Innovation/State Agency of Research MCIN/AEI/10.13039/501100011033, and the MITECO (grant 2552/2020) and National Parks Network (grant 2552/2020). EG-L and MB were supported by the fellowships PTA2016-12325-I and FJCI2017-31725 provided by the MCIN/AEI/10.13039/501100011033.With funding from the Spanish government through the ‘María de Maeztu Unit of Excelence’ accreditation (MDM-2017-0737).Peer reviewedFrontiers MediaMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Ministerio para la Transición Ecológica y el Reto Demográfico (España)Organismo Autónomo Parques Nacionales (España)Ministerio de Economía y Competitividad (España)Moreno Caballud, Ana [0000-0001-7357-584X]Bartolomé, Miguel [0000-0002-2924-6261]Cid, Cristina [0000-0001-5128-4558]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/345373reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104205GB-C22info:eu-repo/grantAgreement/AEI//MDM-2017-0737info:eu-repo/grantAgreement/AEI//PTA2016-12325-Iinfo:eu-repo/grantAgreement/AEI//FJCI2017-31725Ruiz-Blas, Fátima ; Muñoz-Hisado, Víctor ; García-López, Eva ; Moreno Caballud, Ana ; Bartolomé, Miguel ; Leunda, Maria ; Martinez-Alonso, Emma ; Alcázar, Alberto ; Cid, Cristina; 2023; "Data_Sheet_1_The hidden microbial ecosystem in the perennial ice from a Pyrenean ice cave.pdf. [Dataset]"; Figshare; version 1; https://doi.org/10.3389/fmicb.2023.1110091.s001Ruiz-Blas, Fátima ; Muñoz-Hisado, Víctor ; García-López, Eva ; Moreno Caballud, Ana ; Bartolomé, Miguel ; Leunda, Maria ; Martinez-Alonso, Emma ; Alcázar, Alberto ; Cid, Cristina; 2023; "Table_2_The hidden microbial ecosystem in the perennial ice from a Pyrenean ice cave.XLSX [Dataset]"; Figshare; version 1; https://doi.org/10.3389/fmicb.2023.1110091.s002Ruiz-Blas, Fátima ; Muñoz-Hisado, Víctor ; García-López, Eva ; Moreno Caballud, Ana ; Bartolomé, Miguel ; Leunda, Maria ; Martinez-Alonso, Emma ; Alcázar, Alberto ; Cid, Cristina; 2023; "Table_3_The hidden microbial ecosystem in the perennial ice from a Pyrenean ice cave.XLSX [Dataset]"; Figshare; version 1; https://doi.org/10.3389/fmicb.2023.1110091.s003Ruiz-Blas, Fátima ; Muñoz-Hisado, Víctor ; García-López, Eva ; Moreno Caballud, Ana ; Bartolomé, Miguel ; Leunda, Maria ; Martinez-Alonso, Emma ; Alcázar, Alberto ; Cid, Cristina; 2023; "Table_4_The hidden microbial ecosystem in the perennial ice from a Pyrenean ice cave.XLSX [Dataset]"; Figshare; version 1; https://doi.org/10.3389/fmicb.2023.1110091.s004https://doi.org/10.3389/fmicb.2023.1110091Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3453732026-05-22T06:33:51Z
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