Glutamine synthetase in legumes: recent advances in enzyme structure and functional genomics

Glutamine synthetase (GS) is the key enzyme involved in the assimilation of ammonia derived either from nitrate reduction, N2 fixation, photorespiration or asparagine breakdown. A small gene family is encoding for different cytosolic (GS1) or plastidic (GS2) isoforms in legumes. We summarize here th...

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Detalles Bibliográficos
Autores: Betti, Marco, García Calderón, Margarita, Pérez Delgado de Torres, Carmen María, Credali, Alfredo, Estivill, Guillermo, Galván Cejudo, Francisco, Vega Piqueres, José María, Márquez Cabeza, Antonio José
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/47221
Acceso en línea:http://hdl.handle.net/11441/47221
https://doi.org/10.3390/ijms13077994
Access Level:acceso abierto
Palabra clave:glutamine synthetase
Lotus japonicus
functional genomics
nitrogen metabolism
Descripción
Sumario:Glutamine synthetase (GS) is the key enzyme involved in the assimilation of ammonia derived either from nitrate reduction, N2 fixation, photorespiration or asparagine breakdown. A small gene family is encoding for different cytosolic (GS1) or plastidic (GS2) isoforms in legumes. We summarize here the recent advances carried out concerning the quaternary structure of GS, as well as the functional relationship existing between GS2 and processes such as nodulation, photorespiration and water stress, in this latter case by means of proline production. Functional genomic analysis using GS2-minus mutant reveals the key role of GS2 in the metabolic control of the plants and, more particularly, in carbon metabolism.