Neuron-Microglia Contact-Dependent Mechanisms Attenuate Methamphetamine-Induced Microglia Reactivity and Enhance Neuronal Plasticity

Exposure to methamphetamine (Meth) has been classically associated with damage to neuronal terminals. However, it is now becoming clear that addiction may also result from the interplay between glial cells and neurons. Recently, we demonstrated that binge Meth administration promotes microgliosis an...

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Detalles Bibliográficos
Autores: Portugal, Camila Cabral, Bravo, Joana, Ribeiro, Ines, Terceiro, Ana Filipa, Andrade, Elva B., Lopes, Igor M., Azevedo, Maria M., Sousa, Mafalda Machado de, Lopes, Catia D. F., Lobo, Andrea C., Canedo, Teresa, Bettencourt Relvas, Joao, Summavielle, Teresa
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/184165
Acceso en línea:https://hdl.handle.net/2445/184165
Access Level:acceso abierto
Palabra clave:Estimulants
Neuròglia
Stimulants
Neuroglia
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spelling Neuron-Microglia Contact-Dependent Mechanisms Attenuate Methamphetamine-Induced Microglia Reactivity and Enhance Neuronal PlasticityPortugal, Camila CabralBravo, JoanaRibeiro, InesTerceiro, Ana FilipaAndrade, Elva B.Lopes, Igor M.Azevedo, Maria M.Sousa, Mafalda Machado deLopes, Catia D. F.Lobo, Andrea C.Canedo, TeresaBettencourt Relvas, JoaoSummavielle, TeresaEstimulantsNeurògliaStimulantsNeurogliaExposure to methamphetamine (Meth) has been classically associated with damage to neuronal terminals. However, it is now becoming clear that addiction may also result from the interplay between glial cells and neurons. Recently, we demonstrated that binge Meth administration promotes microgliosis and microglia pro-inflammation via astrocytic glutamate release in a TNF/IP(3)R2-Ca2+-dependent manner. Here, we investigated the contribution of neuronal cells to this process. As the crosstalk between microglia and neurons may occur by contact-dependent and/or contact-independent mechanisms, we developed co-cultures of primary neurons and microglia in microfluidic devices to investigate how their interaction affects Meth-induced microglia activation. Our results show that neurons exposed to Meth do not activate microglia in a cell-autonomous way but require astrocyte mediation. Importantly, we found that neurons can partially prevent Meth-induced microglia activation via astrocytes, which seems to be achieved by increasing arginase 1 expression and strengthening the CD200/CD200r pathway. We also observed an increase in synaptic individual area, as determined by co-localization of pre- and post-synaptic markers. The present study provides evidence that contact-dependent mechanisms between neurons and microglia can attenuate pro-inflammatory events such as Meth-induced microglia activation.NLM (Medline)2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/184165Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/cells11030355Cells, 2022, vol 11, num 3https://doi.org/10.3390/cells11030355cc by (c) Portugal, Camila Cabral et al., 2022http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1841652026-05-27T06:46:51Z
dc.title.none.fl_str_mv Neuron-Microglia Contact-Dependent Mechanisms Attenuate Methamphetamine-Induced Microglia Reactivity and Enhance Neuronal Plasticity
title Neuron-Microglia Contact-Dependent Mechanisms Attenuate Methamphetamine-Induced Microglia Reactivity and Enhance Neuronal Plasticity
spellingShingle Neuron-Microglia Contact-Dependent Mechanisms Attenuate Methamphetamine-Induced Microglia Reactivity and Enhance Neuronal Plasticity
Portugal, Camila Cabral
Estimulants
Neuròglia
Stimulants
Neuroglia
title_short Neuron-Microglia Contact-Dependent Mechanisms Attenuate Methamphetamine-Induced Microglia Reactivity and Enhance Neuronal Plasticity
title_full Neuron-Microglia Contact-Dependent Mechanisms Attenuate Methamphetamine-Induced Microglia Reactivity and Enhance Neuronal Plasticity
title_fullStr Neuron-Microglia Contact-Dependent Mechanisms Attenuate Methamphetamine-Induced Microglia Reactivity and Enhance Neuronal Plasticity
title_full_unstemmed Neuron-Microglia Contact-Dependent Mechanisms Attenuate Methamphetamine-Induced Microglia Reactivity and Enhance Neuronal Plasticity
title_sort Neuron-Microglia Contact-Dependent Mechanisms Attenuate Methamphetamine-Induced Microglia Reactivity and Enhance Neuronal Plasticity
dc.creator.none.fl_str_mv Portugal, Camila Cabral
Bravo, Joana
Ribeiro, Ines
Terceiro, Ana Filipa
Andrade, Elva B.
Lopes, Igor M.
Azevedo, Maria M.
Sousa, Mafalda Machado de
Lopes, Catia D. F.
Lobo, Andrea C.
Canedo, Teresa
Bettencourt Relvas, Joao
Summavielle, Teresa
author Portugal, Camila Cabral
author_facet Portugal, Camila Cabral
Bravo, Joana
Ribeiro, Ines
Terceiro, Ana Filipa
Andrade, Elva B.
Lopes, Igor M.
Azevedo, Maria M.
Sousa, Mafalda Machado de
Lopes, Catia D. F.
Lobo, Andrea C.
Canedo, Teresa
Bettencourt Relvas, Joao
Summavielle, Teresa
author_role author
author2 Bravo, Joana
Ribeiro, Ines
Terceiro, Ana Filipa
Andrade, Elva B.
Lopes, Igor M.
Azevedo, Maria M.
Sousa, Mafalda Machado de
Lopes, Catia D. F.
Lobo, Andrea C.
Canedo, Teresa
Bettencourt Relvas, Joao
Summavielle, Teresa
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Estimulants
Neuròglia
Stimulants
Neuroglia
topic Estimulants
Neuròglia
Stimulants
Neuroglia
description Exposure to methamphetamine (Meth) has been classically associated with damage to neuronal terminals. However, it is now becoming clear that addiction may also result from the interplay between glial cells and neurons. Recently, we demonstrated that binge Meth administration promotes microgliosis and microglia pro-inflammation via astrocytic glutamate release in a TNF/IP(3)R2-Ca2+-dependent manner. Here, we investigated the contribution of neuronal cells to this process. As the crosstalk between microglia and neurons may occur by contact-dependent and/or contact-independent mechanisms, we developed co-cultures of primary neurons and microglia in microfluidic devices to investigate how their interaction affects Meth-induced microglia activation. Our results show that neurons exposed to Meth do not activate microglia in a cell-autonomous way but require astrocyte mediation. Importantly, we found that neurons can partially prevent Meth-induced microglia activation via astrocytes, which seems to be achieved by increasing arginase 1 expression and strengthening the CD200/CD200r pathway. We also observed an increase in synaptic individual area, as determined by co-localization of pre- and post-synaptic markers. The present study provides evidence that contact-dependent mechanisms between neurons and microglia can attenuate pro-inflammatory events such as Meth-induced microglia activation.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/184165
url https://hdl.handle.net/2445/184165
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/cells11030355
Cells, 2022, vol 11, num 3
https://doi.org/10.3390/cells11030355
dc.rights.none.fl_str_mv cc by (c) Portugal, Camila Cabral et al., 2022
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Portugal, Camila Cabral et al., 2022
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv NLM (Medline)
publisher.none.fl_str_mv NLM (Medline)
dc.source.none.fl_str_mv Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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