Inhibitory Effect on In Vitro Streptococcus oralis Biofilm of a Soda-Lime Glass Containing Silver Nanoparticles Coating on Titanium Alloy

This paper reports the effect of soda-lime-glass-nAg coating on the viability of an in vitro biofilm of Streptococcus oralis. Three strains (ATCC 35037 and two clinical isolates from periodontitis patients) were grown on coated with glass, glass containing silver nanoparticles, and uncoated titanium...

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Detalles Bibliográficos
Autores: Cabal, Belén, Cafini, Fabio, Esteban-Tejeda, Leticia, Alou Cervera, Luis, Bartolomé, José F., Sevillano Fernández, David, López-Piriz, Roberto, Torrecillas, Ramón, Serafín Moya, José
Tipo de recurso: artículo
Fecha de publicación:2012
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/107044
Acceso en línea:https://hdl.handle.net/20.500.14352/107044
Access Level:acceso abierto
Palabra clave:611.02
616.314
Materiales dentales
Microbiología médica
2414 Microbiología
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oai_identifier_str oai:docta.ucm.es:20.500.14352/107044
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spelling Inhibitory Effect on In Vitro Streptococcus oralis Biofilm of a Soda-Lime Glass Containing Silver Nanoparticles Coating on Titanium AlloyCabal, BelénCafini, FabioEsteban-Tejeda, LeticiaAlou Cervera, LuisBartolomé, José F.Sevillano Fernández, DavidLópez-Piriz, RobertoTorrecillas, RamónSerafín Moya, José611.02616.314Materiales dentalesMicrobiología médica2414 MicrobiologíaThis paper reports the effect of soda-lime-glass-nAg coating on the viability of an in vitro biofilm of Streptococcus oralis. Three strains (ATCC 35037 and two clinical isolates from periodontitis patients) were grown on coated with glass, glass containing silver nanoparticles, and uncoated titanium alloy disks. Two different methods were used to quantify biofilm formation abilities: crystal violet staining and determination of viable counts. The influence of the surface morphology on the cell attachment was studied. The surface morphology was characterized by scanning electron microscopy (SEM) and using a profilometer. SEM was also used to study the formation and the development of biofilm on the coated and uncoated disks. At least a >99.7% inocula reduction of biofilm respect to titanium disks and also to glass coated disks was observed in the glass-nAg coated disks for all the studied strains. A quantitative evaluation of the release of silver was conducted in vitro to test whether and to what extend the biocidal agent (silver) could leach from the coating. These findings suggest that the biofilm formation of S. oralis strains is highly inhibited by the glass-nAg and may be useful for materials which require durable antibacterial effect on their surfaces, as it is the case of dental implants.PLOSWei-Chun ChinUniversidad Complutense de Madrid20122012-08-0320122012-08-03journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/107044reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-ShareAlike 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1070442026-06-02T12:44:21Z
dc.title.none.fl_str_mv Inhibitory Effect on In Vitro Streptococcus oralis Biofilm of a Soda-Lime Glass Containing Silver Nanoparticles Coating on Titanium Alloy
title Inhibitory Effect on In Vitro Streptococcus oralis Biofilm of a Soda-Lime Glass Containing Silver Nanoparticles Coating on Titanium Alloy
spellingShingle Inhibitory Effect on In Vitro Streptococcus oralis Biofilm of a Soda-Lime Glass Containing Silver Nanoparticles Coating on Titanium Alloy
Cabal, Belén
611.02
616.314
Materiales dentales
Microbiología médica
2414 Microbiología
title_short Inhibitory Effect on In Vitro Streptococcus oralis Biofilm of a Soda-Lime Glass Containing Silver Nanoparticles Coating on Titanium Alloy
title_full Inhibitory Effect on In Vitro Streptococcus oralis Biofilm of a Soda-Lime Glass Containing Silver Nanoparticles Coating on Titanium Alloy
title_fullStr Inhibitory Effect on In Vitro Streptococcus oralis Biofilm of a Soda-Lime Glass Containing Silver Nanoparticles Coating on Titanium Alloy
title_full_unstemmed Inhibitory Effect on In Vitro Streptococcus oralis Biofilm of a Soda-Lime Glass Containing Silver Nanoparticles Coating on Titanium Alloy
title_sort Inhibitory Effect on In Vitro Streptococcus oralis Biofilm of a Soda-Lime Glass Containing Silver Nanoparticles Coating on Titanium Alloy
dc.creator.none.fl_str_mv Cabal, Belén
Cafini, Fabio
Esteban-Tejeda, Leticia
Alou Cervera, Luis
Bartolomé, José F.
Sevillano Fernández, David
López-Piriz, Roberto
Torrecillas, Ramón
Serafín Moya, José
author Cabal, Belén
author_facet Cabal, Belén
Cafini, Fabio
Esteban-Tejeda, Leticia
Alou Cervera, Luis
Bartolomé, José F.
Sevillano Fernández, David
López-Piriz, Roberto
Torrecillas, Ramón
Serafín Moya, José
author_role author
author2 Cafini, Fabio
Esteban-Tejeda, Leticia
Alou Cervera, Luis
Bartolomé, José F.
Sevillano Fernández, David
López-Piriz, Roberto
Torrecillas, Ramón
Serafín Moya, José
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Wei-Chun Chin
Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 611.02
616.314
Materiales dentales
Microbiología médica
2414 Microbiología
topic 611.02
616.314
Materiales dentales
Microbiología médica
2414 Microbiología
description This paper reports the effect of soda-lime-glass-nAg coating on the viability of an in vitro biofilm of Streptococcus oralis. Three strains (ATCC 35037 and two clinical isolates from periodontitis patients) were grown on coated with glass, glass containing silver nanoparticles, and uncoated titanium alloy disks. Two different methods were used to quantify biofilm formation abilities: crystal violet staining and determination of viable counts. The influence of the surface morphology on the cell attachment was studied. The surface morphology was characterized by scanning electron microscopy (SEM) and using a profilometer. SEM was also used to study the formation and the development of biofilm on the coated and uncoated disks. At least a >99.7% inocula reduction of biofilm respect to titanium disks and also to glass coated disks was observed in the glass-nAg coated disks for all the studied strains. A quantitative evaluation of the release of silver was conducted in vitro to test whether and to what extend the biocidal agent (silver) could leach from the coating. These findings suggest that the biofilm formation of S. oralis strains is highly inhibited by the glass-nAg and may be useful for materials which require durable antibacterial effect on their surfaces, as it is the case of dental implants.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-08-03
2012
2012-08-03
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/107044
url https://hdl.handle.net/20.500.14352/107044
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-ShareAlike 4.0 International
http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-ShareAlike 4.0 International
http://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv PLOS
publisher.none.fl_str_mv PLOS
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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