DDC expression is not regulated by NFAT5 (TonEBP) in dopaminergic neural cell lines upon hypertonic stress

The nuclear factor of activated T-cells 5 (NFAT5), also known as tonicity-responsive enhancer-binding protein (TonEBP), is a transcription factor that regulates osmoadaptive response in multiple tissues and is highly expressed in the developing central nervous system. A former study reported that NF...

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Authors: Pineda-Cirera, L., Cabana Domínguez, Judit, Benetó, N., Díeze, H., Arenas Solà, Concepción, Cormand Rifà, Bru, Fernàndez Castillo, Noèlia
Format: article
Status:Versión aceptada para publicación
Publication Date:2020
Country:España
Institution:Universidad de Barcelona
Repository:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/207611
Online Access:https://hdl.handle.net/2445/207611
Access Level:Open access
Keyword:Xarxes neuronals (Neurobiologia)
Cèl·lules T
Circuit neuronal
Neural networks (Neurobiology)
T cells
Neural circuitry
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oai_identifier_str oai:diposit.ub.edu:2445/207611
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repository_id_str
spelling DDC expression is not regulated by NFAT5 (TonEBP) in dopaminergic neural cell lines upon hypertonic stressPineda-Cirera, L.Cabana Domínguez, JuditBenetó, N.Díeze, H.Arenas Solà, ConcepciónCormand Rifà, BruFernàndez Castillo, NoèliaXarxes neuronals (Neurobiologia)Cèl·lules TCircuit neuronalNeural networks (Neurobiology)T cellsNeural circuitryThe nuclear factor of activated T-cells 5 (NFAT5), also known as tonicity-responsive enhancer-binding protein (TonEBP), is a transcription factor that regulates osmoadaptive response in multiple tissues and is highly expressed in the developing central nervous system. A former study reported that NFAT5 activation through hypertonic stress increases the expression of the dopa decarboxylase enzyme (DDC), also known as aromatic-l-amino-acid decarboxylase (AADC), in human renal proximal tubule cells, leading to an increase of dopamine synthesis. In a previous study, we identified NFAT5 as a candidate gene for cocaine dependence, a complex psychiatric disorder in which dopaminergic neurotransmission plays an important role. Therefore, to test the hypothesis that NFAT5 may also affect dopamine levels in the nervous system through the regulation of DDC expression, we examined this regulation using two neural dopaminergic cell lines, SH-SY5Y and PC12. The effect of NFAT5 on the expression of the neuronal isoform of DDC was evaluated by qRT-PCR. Upon hypertonic stress, NFAT5 was activated and accumulated into the nuclei and, subsequently, the expression of NFAT5 and its known targets sodium/myo-inositol cotransporter 1 (SMIT) and sodium chloride/taurine cotransporter (TAUT) increased, as expected. However, the expression of DDC decreased. When silencing the expression of NFAT5 with a specific shRNA we observed that the downregulation of DDC is independent from NFAT5 in both cell lines and is due to hypertonic stress. In conclusion, NFAT5 does not regulate the expression of the neuronal isoform of DDC in neural dopaminergic cell lines and, consequently, it does not modulate dopamine synthesis through DDC.Elsevier B.V.2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/207611Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1016/j.gene.2020.144569Gene, 2020, vol. 742https://doi.org/10.1016/j.gene.2020.144569cc-by-nc-nd (c) Elsevier B.V., 2020http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2076112026-05-27T06:46:51Z
dc.title.none.fl_str_mv DDC expression is not regulated by NFAT5 (TonEBP) in dopaminergic neural cell lines upon hypertonic stress
title DDC expression is not regulated by NFAT5 (TonEBP) in dopaminergic neural cell lines upon hypertonic stress
spellingShingle DDC expression is not regulated by NFAT5 (TonEBP) in dopaminergic neural cell lines upon hypertonic stress
Pineda-Cirera, L.
Xarxes neuronals (Neurobiologia)
Cèl·lules T
Circuit neuronal
Neural networks (Neurobiology)
T cells
Neural circuitry
title_short DDC expression is not regulated by NFAT5 (TonEBP) in dopaminergic neural cell lines upon hypertonic stress
title_full DDC expression is not regulated by NFAT5 (TonEBP) in dopaminergic neural cell lines upon hypertonic stress
title_fullStr DDC expression is not regulated by NFAT5 (TonEBP) in dopaminergic neural cell lines upon hypertonic stress
title_full_unstemmed DDC expression is not regulated by NFAT5 (TonEBP) in dopaminergic neural cell lines upon hypertonic stress
title_sort DDC expression is not regulated by NFAT5 (TonEBP) in dopaminergic neural cell lines upon hypertonic stress
dc.creator.none.fl_str_mv Pineda-Cirera, L.
Cabana Domínguez, Judit
Benetó, N.
Díeze, H.
Arenas Solà, Concepción
Cormand Rifà, Bru
Fernàndez Castillo, Noèlia
author Pineda-Cirera, L.
author_facet Pineda-Cirera, L.
Cabana Domínguez, Judit
Benetó, N.
Díeze, H.
Arenas Solà, Concepción
Cormand Rifà, Bru
Fernàndez Castillo, Noèlia
author_role author
author2 Cabana Domínguez, Judit
Benetó, N.
Díeze, H.
Arenas Solà, Concepción
Cormand Rifà, Bru
Fernàndez Castillo, Noèlia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Xarxes neuronals (Neurobiologia)
Cèl·lules T
Circuit neuronal
Neural networks (Neurobiology)
T cells
Neural circuitry
topic Xarxes neuronals (Neurobiologia)
Cèl·lules T
Circuit neuronal
Neural networks (Neurobiology)
T cells
Neural circuitry
description The nuclear factor of activated T-cells 5 (NFAT5), also known as tonicity-responsive enhancer-binding protein (TonEBP), is a transcription factor that regulates osmoadaptive response in multiple tissues and is highly expressed in the developing central nervous system. A former study reported that NFAT5 activation through hypertonic stress increases the expression of the dopa decarboxylase enzyme (DDC), also known as aromatic-l-amino-acid decarboxylase (AADC), in human renal proximal tubule cells, leading to an increase of dopamine synthesis. In a previous study, we identified NFAT5 as a candidate gene for cocaine dependence, a complex psychiatric disorder in which dopaminergic neurotransmission plays an important role. Therefore, to test the hypothesis that NFAT5 may also affect dopamine levels in the nervous system through the regulation of DDC expression, we examined this regulation using two neural dopaminergic cell lines, SH-SY5Y and PC12. The effect of NFAT5 on the expression of the neuronal isoform of DDC was evaluated by qRT-PCR. Upon hypertonic stress, NFAT5 was activated and accumulated into the nuclei and, subsequently, the expression of NFAT5 and its known targets sodium/myo-inositol cotransporter 1 (SMIT) and sodium chloride/taurine cotransporter (TAUT) increased, as expected. However, the expression of DDC decreased. When silencing the expression of NFAT5 with a specific shRNA we observed that the downregulation of DDC is independent from NFAT5 in both cell lines and is due to hypertonic stress. In conclusion, NFAT5 does not regulate the expression of the neuronal isoform of DDC in neural dopaminergic cell lines and, consequently, it does not modulate dopamine synthesis through DDC.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/207611
url https://hdl.handle.net/2445/207611
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.gene.2020.144569
Gene, 2020, vol. 742
https://doi.org/10.1016/j.gene.2020.144569
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Elsevier B.V., 2020
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Elsevier B.V., 2020
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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