Metadata for RNAseq project analysing differential expression in Culex pipiens mosquitoes infected by two avian Plasmodium species
[Description of methods used for collection/generation of data] We collected birds infected by Plasmodium cathemerium and Plasmodium relictum and uninfected birds. We collected mosquito larvae that were raised in the lab. Adult mosquitoes were divided in 3 groups allowed them to feed overnight on a...
| Authors: | , , , , |
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| Format: | conjunto de datos |
| Publication Date: | 2021 |
| Country: | España |
| Institution: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repository: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/340499 |
| Online Access: | http://hdl.handle.net/10261/340499 https://doi.org/10.20350/digitalCSIC/15708 |
| Access Level: | Open access |
| Keyword: | Transcriptomes Avian malaria Culex Gene expresion |
| Summary: | [Description of methods used for collection/generation of data] We collected birds infected by Plasmodium cathemerium and Plasmodium relictum and uninfected birds. We collected mosquito larvae that were raised in the lab. Adult mosquitoes were divided in 3 groups allowed them to feed overnight on a P. relictum-infected bird, a P. cathemerium-infected bird and an uninfected control bird. For transcriptome analyses, we processed mosquitoes at three time points after exposure, 24 hours post-infection (hpi), 10 days post-infection (dpi) and 21 dpi. At each time point, we created pools of 5 mosquitoes of each infection status capturing the mosquitoes alive and immediately transferring them to dry ice. We preserved the mosquitoes at -80ºC until RNA extractions were carried out. We collected a total of 36 samples including controls (4 pools x 3 time points x 3 conditions). We extracted RNA and DNA from pools of 5 mosquitoes using TRIzol® (Invitrogen, Carlsbad, CA, USA) followed by column purification using RNeasy mini kit® (QIAGEN, Hilden, Germany). RNA samples were submitted to the Polo d’Innovazione di Genomica, Genetica e Biologia, Siena (Italy) where library preparation and sequencing were carried out. |
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