High Redox Potential Cathode Based on Laccase Covalently Attached to Gold Electrode

A new strategy for oriented covalent immobilization of Trametes hirsuta laccase on gold electrodes is presented. The strategy is based on the gold surface modification with a mixed monolayer of an aromatic diazonium salt derivative and 6-mercapto-1-hexanol for further use as scaffold for the enzyme’...

Descripción completa

Detalles Bibliográficos
Autores: Pita, Marcos, Gutiérrez-Sánchez, Cristina, Olea, David, Vélez, Marisela, García Diego, Cristina, Shleev, Sergey, Fernández López, Víctor Manuel, López de Lacey, Antonio
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/54208
Acceso en línea:http://hdl.handle.net/10261/54208
Access Level:acceso abierto
Palabra clave:Laccase
Gold
Biocathode
Diazonium salt
Direct electron transfer
id ES_fd9c6a68a39612b7ec7e493a4e4bbc7a
oai_identifier_str oai:digital.csic.es:10261/54208
network_acronym_str ES
network_name_str España
repository_id_str
spelling High Redox Potential Cathode Based on Laccase Covalently Attached to Gold ElectrodePita, MarcosGutiérrez-Sánchez, CristinaOlea, DavidVélez, MariselaGarcía Diego, CristinaShleev, SergeyFernández López, Víctor ManuelLópez de Lacey, AntonioLaccaseGoldBiocathodeDiazonium saltDirect electron transferA new strategy for oriented covalent immobilization of Trametes hirsuta laccase on gold electrodes is presented. The strategy is based on the gold surface modification with a mixed monolayer of an aromatic diazonium salt derivative and 6-mercapto-1-hexanol for further use as scaffold for the enzyme’s covalent linkage. This strategy offers a variety of advantages such as high stability and laccase-friendly support morphology, which turns it into a suitable metal-enzyme interface. Conditions aiming at optimum orientation for direct electron transfer (DET) via the T1 copper site were studied. Current density values up to 40 μA·cm-2 were measured for the electrocatalytic reduction of O2 in absence of redox mediators. This strategy is a big step forward in the development of laccase-modified gold electrodes for bioelectrocatalytic reduction of O2.This work is funded by the European Research Council, project 3D-NanoBioDevice NMP4-SL-2009-229255 and the Spanish MICINN, and project CTQ2009-12649. Dr. M. Pita acknowledges the Ramon y Cajal 2009 program from the Spanish MICINN. Dr. D. Olea acknowledges the JAE program from CSIC.Peer reviewedAmerican Chemical SocietyEuropean Research CouncilConsejo Superior de Investigaciones Científicas (España)Ministerio de Ciencia e Innovación (España)201220122011info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/54208reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/FP7/229255http://doi.org/10.1021/jp203643hinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/542082026-05-22T06:33:51Z
dc.title.none.fl_str_mv High Redox Potential Cathode Based on Laccase Covalently Attached to Gold Electrode
title High Redox Potential Cathode Based on Laccase Covalently Attached to Gold Electrode
spellingShingle High Redox Potential Cathode Based on Laccase Covalently Attached to Gold Electrode
Pita, Marcos
Laccase
Gold
Biocathode
Diazonium salt
Direct electron transfer
title_short High Redox Potential Cathode Based on Laccase Covalently Attached to Gold Electrode
title_full High Redox Potential Cathode Based on Laccase Covalently Attached to Gold Electrode
title_fullStr High Redox Potential Cathode Based on Laccase Covalently Attached to Gold Electrode
title_full_unstemmed High Redox Potential Cathode Based on Laccase Covalently Attached to Gold Electrode
title_sort High Redox Potential Cathode Based on Laccase Covalently Attached to Gold Electrode
dc.creator.none.fl_str_mv Pita, Marcos
Gutiérrez-Sánchez, Cristina
Olea, David
Vélez, Marisela
García Diego, Cristina
Shleev, Sergey
Fernández López, Víctor Manuel
López de Lacey, Antonio
author Pita, Marcos
author_facet Pita, Marcos
Gutiérrez-Sánchez, Cristina
Olea, David
Vélez, Marisela
García Diego, Cristina
Shleev, Sergey
Fernández López, Víctor Manuel
López de Lacey, Antonio
author_role author
author2 Gutiérrez-Sánchez, Cristina
Olea, David
Vélez, Marisela
García Diego, Cristina
Shleev, Sergey
Fernández López, Víctor Manuel
López de Lacey, Antonio
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Research Council
Consejo Superior de Investigaciones Científicas (España)
Ministerio de Ciencia e Innovación (España)
dc.subject.none.fl_str_mv Laccase
Gold
Biocathode
Diazonium salt
Direct electron transfer
topic Laccase
Gold
Biocathode
Diazonium salt
Direct electron transfer
description A new strategy for oriented covalent immobilization of Trametes hirsuta laccase on gold electrodes is presented. The strategy is based on the gold surface modification with a mixed monolayer of an aromatic diazonium salt derivative and 6-mercapto-1-hexanol for further use as scaffold for the enzyme’s covalent linkage. This strategy offers a variety of advantages such as high stability and laccase-friendly support morphology, which turns it into a suitable metal-enzyme interface. Conditions aiming at optimum orientation for direct electron transfer (DET) via the T1 copper site were studied. Current density values up to 40 μA·cm-2 were measured for the electrocatalytic reduction of O2 in absence of redox mediators. This strategy is a big step forward in the development of laccase-modified gold electrodes for bioelectrocatalytic reduction of O2.
publishDate 2011
dc.date.none.fl_str_mv 2011
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/54208
url http://hdl.handle.net/10261/54208
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/FP7/229255
http://doi.org/10.1021/jp203643h
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869425582082621440
score 15.811543

Similares en LA Referencia