Human selenoprotein P and S variant mRNAs with different numbers of SECIS elements and inferences from mutant mice of the roles of multiple SECIS elements
Dynamic redefinition of the 10 UGAs in human and mouse selenoprotein P (Sepp1) mRNAs to specify selenocysteine instead of termination involves two 3′ UTR structural elements (SECIS) and is regulated by selenium availability. In addition to the previously known human Sepp1 mRNA poly(A) addition site...
| Autores: | , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:10230/69437 |
| Acceso en línea: | http://hdl.handle.net/10230/69437 http://dx.doi.org/10.1098/rsob.160241 |
| Access Level: | acceso abierto |
| Palabra clave: | Codon redefinition Ribosome specialization Selenocysteine Selenoprotein P Selenoprotein S |
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Human selenoprotein P and S variant mRNAs with different numbers of SECIS elements and inferences from mutant mice of the roles of multiple SECIS elementsWu, SenMariotti, Marco, 1984-Santesmasses Ruiz, Didac, 1978-Hill, Kristina E.Baclaocos, JaninahAparicio i Prat, Estel, 1986-Li, ShupingMackrill, John J.Wu, YuanyuanHoward, Michael T.Capecchi, MarioGuigó Serra, RodericBurk, Raymond F.Atkins, John F.Codon redefinitionRibosome specializationSelenocysteineSelenoprotein PSelenoprotein SDynamic redefinition of the 10 UGAs in human and mouse selenoprotein P (Sepp1) mRNAs to specify selenocysteine instead of termination involves two 3′ UTR structural elements (SECIS) and is regulated by selenium availability. In addition to the previously known human Sepp1 mRNA poly(A) addition site just 3′ of SECIS 2, two further sites were identified with one resulting in 10–25% of the mRNA lacking SECIS 2. To address function, mutant mice were generated with either SECIS 1 or SECIS 2 deleted or with the first UGA substituted with a serine codon. They were fed on either high or selenium-deficient diets. The mutants had very different effects on the proportions of shorter and longer product Sepp1 protein isoforms isolated from plasma, and on viability. Spatially and functionally distinctive effects of the two SECIS elements on UGA decoding were inferred. We also bioinformatically identify two selenoprotein S mRNAs with different 5′ sequences predicted to yield products with different N-termini. These results provide insights into SECIS function and mRNA processing in selenoprotein isoform diversity.Royal Society202520252016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/69437http://dx.doi.org/10.1098/rsob.160241reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésOpen Biology. 2016 Nov;6(11):160241© 2016 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/694372026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Human selenoprotein P and S variant mRNAs with different numbers of SECIS elements and inferences from mutant mice of the roles of multiple SECIS elements |
| title |
Human selenoprotein P and S variant mRNAs with different numbers of SECIS elements and inferences from mutant mice of the roles of multiple SECIS elements |
| spellingShingle |
Human selenoprotein P and S variant mRNAs with different numbers of SECIS elements and inferences from mutant mice of the roles of multiple SECIS elements Wu, Sen Codon redefinition Ribosome specialization Selenocysteine Selenoprotein P Selenoprotein S |
| title_short |
Human selenoprotein P and S variant mRNAs with different numbers of SECIS elements and inferences from mutant mice of the roles of multiple SECIS elements |
| title_full |
Human selenoprotein P and S variant mRNAs with different numbers of SECIS elements and inferences from mutant mice of the roles of multiple SECIS elements |
| title_fullStr |
Human selenoprotein P and S variant mRNAs with different numbers of SECIS elements and inferences from mutant mice of the roles of multiple SECIS elements |
| title_full_unstemmed |
Human selenoprotein P and S variant mRNAs with different numbers of SECIS elements and inferences from mutant mice of the roles of multiple SECIS elements |
| title_sort |
Human selenoprotein P and S variant mRNAs with different numbers of SECIS elements and inferences from mutant mice of the roles of multiple SECIS elements |
| dc.creator.none.fl_str_mv |
Wu, Sen Mariotti, Marco, 1984- Santesmasses Ruiz, Didac, 1978- Hill, Kristina E. Baclaocos, Janinah Aparicio i Prat, Estel, 1986- Li, Shuping Mackrill, John J. Wu, Yuanyuan Howard, Michael T. Capecchi, Mario Guigó Serra, Roderic Burk, Raymond F. Atkins, John F. |
| author |
Wu, Sen |
| author_facet |
Wu, Sen Mariotti, Marco, 1984- Santesmasses Ruiz, Didac, 1978- Hill, Kristina E. Baclaocos, Janinah Aparicio i Prat, Estel, 1986- Li, Shuping Mackrill, John J. Wu, Yuanyuan Howard, Michael T. Capecchi, Mario Guigó Serra, Roderic Burk, Raymond F. Atkins, John F. |
| author_role |
author |
| author2 |
Mariotti, Marco, 1984- Santesmasses Ruiz, Didac, 1978- Hill, Kristina E. Baclaocos, Janinah Aparicio i Prat, Estel, 1986- Li, Shuping Mackrill, John J. Wu, Yuanyuan Howard, Michael T. Capecchi, Mario Guigó Serra, Roderic Burk, Raymond F. Atkins, John F. |
| author2_role |
author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Codon redefinition Ribosome specialization Selenocysteine Selenoprotein P Selenoprotein S |
| topic |
Codon redefinition Ribosome specialization Selenocysteine Selenoprotein P Selenoprotein S |
| description |
Dynamic redefinition of the 10 UGAs in human and mouse selenoprotein P (Sepp1) mRNAs to specify selenocysteine instead of termination involves two 3′ UTR structural elements (SECIS) and is regulated by selenium availability. In addition to the previously known human Sepp1 mRNA poly(A) addition site just 3′ of SECIS 2, two further sites were identified with one resulting in 10–25% of the mRNA lacking SECIS 2. To address function, mutant mice were generated with either SECIS 1 or SECIS 2 deleted or with the first UGA substituted with a serine codon. They were fed on either high or selenium-deficient diets. The mutants had very different effects on the proportions of shorter and longer product Sepp1 protein isoforms isolated from plasma, and on viability. Spatially and functionally distinctive effects of the two SECIS elements on UGA decoding were inferred. We also bioinformatically identify two selenoprotein S mRNAs with different 5′ sequences predicted to yield products with different N-termini. These results provide insights into SECIS function and mRNA processing in selenoprotein isoform diversity. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016 2025 2025 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10230/69437 http://dx.doi.org/10.1098/rsob.160241 |
| url |
http://hdl.handle.net/10230/69437 http://dx.doi.org/10.1098/rsob.160241 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Open Biology. 2016 Nov;6(11):160241 |
| dc.rights.none.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Royal Society |
| publisher.none.fl_str_mv |
Royal Society |
| dc.source.none.fl_str_mv |
reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Recercat. Dipósit de la Recerca de Catalunya |
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Recercat. Dipósit de la Recerca de Catalunya |
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