QTL mapping of melon fruit quality traits using a high-density GBS-based genetic map

Background: Melon shows a broad diversity in fruit morphology and quality, which is still underexploited in breeding programs. The knowledge of the genetic basis of fruit quality traits is important for identifying new alleles that may be introduced in elite material by highly efficient molecular br...

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Autores: Pereira, Lara|||0000-0001-5184-8587, Ruggieri, Valentino|||0000-0001-6038-8703, Pérez, S., Alexiou, Konstantinos G.|||0000-0003-0121-9517, Fernández, Marta, Jahrmann, Torben, Pujol Abajo, Marta|||0000-0002-3595-5363, Garcia-Mas, Jordi|||0000-0001-7101-9049
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:218127
Acceso en línea:https://ddd.uab.cat/record/218127
https://dx.doi.org/urn:doi:10.1186/s12870-018-1537-5
Access Level:acceso abierto
Palabra clave:Quantitative trait loci
Melon
Fruit quality
Fruit morphology
Genotyping-by-sequencing
Genetic map
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spelling QTL mapping of melon fruit quality traits using a high-density GBS-based genetic mapPereira, Lara|||0000-0001-5184-8587Ruggieri, Valentino|||0000-0001-6038-8703Pérez, S.Alexiou, Konstantinos G.|||0000-0003-0121-9517Fernández, MartaJahrmann, TorbenPujol Abajo, Marta|||0000-0002-3595-5363Garcia-Mas, Jordi|||0000-0001-7101-9049Quantitative trait lociMelonFruit qualityFruit morphologyGenotyping-by-sequencingGenetic mapBackground: Melon shows a broad diversity in fruit morphology and quality, which is still underexploited in breeding programs. The knowledge of the genetic basis of fruit quality traits is important for identifying new alleles that may be introduced in elite material by highly efficient molecular breeding tools. - Results: In order to identify QTLs controlling fruit quality, a recombinant inbred line population was developed using two commercial cultivars as parental lines: "Védrantais", from the cantalupensis group, and "Piel de Sapo", from the inodorus group. Both have desirable quality traits for the market, but their fruits differ in traits such as rind and flesh color, sugar content, ripening behavior, size and shape. We used a genotyping-by-sequencing strategy to construct a dense genetic map, which included around five thousand variants distributed in 824 bins. The RIL population was phenotyped for quality and morphology traits, and we mapped 33 stable QTLs involved in sugar and carotenoid content, fruit and seed morphology and major loci controlling external color of immature fruit and mottled rind. The median confidence interval of the QTLs was 942kb, suggesting that the high density of the genetic map helped in increasing the mapping resolution. Some of these intervals contained less than a hundred annotated genes, and an integrative strategy combining gene expression and resequencing data enabled identification of candidate genes for some of these traits. - Conclusion: Several QTLs controlling fruit quality traits in melon were identified and delimited to narrow genomic intervals, using a RIL population and a GBS-based genetic map. 22018-01-0120182018-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/218127https://dx.doi.org/urn:doi:10.1186/s12870-018-1537-5reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengEuropean Commission https://doi.org/10.13039/501100000780 6655919Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 AGL2015-64625-C2-1-RMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 SEV-2015-0533open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2181272026-06-06T12:50:31Z
dc.title.none.fl_str_mv QTL mapping of melon fruit quality traits using a high-density GBS-based genetic map
title QTL mapping of melon fruit quality traits using a high-density GBS-based genetic map
spellingShingle QTL mapping of melon fruit quality traits using a high-density GBS-based genetic map
Pereira, Lara|||0000-0001-5184-8587
Quantitative trait loci
Melon
Fruit quality
Fruit morphology
Genotyping-by-sequencing
Genetic map
title_short QTL mapping of melon fruit quality traits using a high-density GBS-based genetic map
title_full QTL mapping of melon fruit quality traits using a high-density GBS-based genetic map
title_fullStr QTL mapping of melon fruit quality traits using a high-density GBS-based genetic map
title_full_unstemmed QTL mapping of melon fruit quality traits using a high-density GBS-based genetic map
title_sort QTL mapping of melon fruit quality traits using a high-density GBS-based genetic map
dc.creator.none.fl_str_mv Pereira, Lara|||0000-0001-5184-8587
Ruggieri, Valentino|||0000-0001-6038-8703
Pérez, S.
Alexiou, Konstantinos G.|||0000-0003-0121-9517
Fernández, Marta
Jahrmann, Torben
Pujol Abajo, Marta|||0000-0002-3595-5363
Garcia-Mas, Jordi|||0000-0001-7101-9049
author Pereira, Lara|||0000-0001-5184-8587
author_facet Pereira, Lara|||0000-0001-5184-8587
Ruggieri, Valentino|||0000-0001-6038-8703
Pérez, S.
Alexiou, Konstantinos G.|||0000-0003-0121-9517
Fernández, Marta
Jahrmann, Torben
Pujol Abajo, Marta|||0000-0002-3595-5363
Garcia-Mas, Jordi|||0000-0001-7101-9049
author_role author
author2 Ruggieri, Valentino|||0000-0001-6038-8703
Pérez, S.
Alexiou, Konstantinos G.|||0000-0003-0121-9517
Fernández, Marta
Jahrmann, Torben
Pujol Abajo, Marta|||0000-0002-3595-5363
Garcia-Mas, Jordi|||0000-0001-7101-9049
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Quantitative trait loci
Melon
Fruit quality
Fruit morphology
Genotyping-by-sequencing
Genetic map
topic Quantitative trait loci
Melon
Fruit quality
Fruit morphology
Genotyping-by-sequencing
Genetic map
description Background: Melon shows a broad diversity in fruit morphology and quality, which is still underexploited in breeding programs. The knowledge of the genetic basis of fruit quality traits is important for identifying new alleles that may be introduced in elite material by highly efficient molecular breeding tools. - Results: In order to identify QTLs controlling fruit quality, a recombinant inbred line population was developed using two commercial cultivars as parental lines: "Védrantais", from the cantalupensis group, and "Piel de Sapo", from the inodorus group. Both have desirable quality traits for the market, but their fruits differ in traits such as rind and flesh color, sugar content, ripening behavior, size and shape. We used a genotyping-by-sequencing strategy to construct a dense genetic map, which included around five thousand variants distributed in 824 bins. The RIL population was phenotyped for quality and morphology traits, and we mapped 33 stable QTLs involved in sugar and carotenoid content, fruit and seed morphology and major loci controlling external color of immature fruit and mottled rind. The median confidence interval of the QTLs was 942kb, suggesting that the high density of the genetic map helped in increasing the mapping resolution. Some of these intervals contained less than a hundred annotated genes, and an integrative strategy combining gene expression and resequencing data enabled identification of candidate genes for some of these traits. - Conclusion: Several QTLs controlling fruit quality traits in melon were identified and delimited to narrow genomic intervals, using a RIL population and a GBS-based genetic map.
publishDate 2018
dc.date.none.fl_str_mv 2
2018-01-01
2018
2018-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/218127
https://dx.doi.org/urn:doi:10.1186/s12870-018-1537-5
url https://ddd.uab.cat/record/218127
https://dx.doi.org/urn:doi:10.1186/s12870-018-1537-5
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission https://doi.org/10.13039/501100000780 6655919
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 AGL2015-64625-C2-1-R
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 SEV-2015-0533
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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