Seminal plasma antioxidants are related to sperm cryotolerance in the horse

The objective of this study was to determine the relationship of enzymatic (superoxide dismutase, SOD; glutathione peroxidase, GPX; catalase, CAT; and paraoxonase type 1, PON1) and non-enzymatic antioxidants (measured in terms of: Trolox equivalent antioxidant capacity, TEAC; cupric-reducing antioxi...

Descripción completa

Detalles Bibliográficos
Autores: Catalán, Jaime, Yáñez-Ortiz, Iván, Tvarijonaviciute, Asta, González-Arostegui, Luis Guillermo, Rubio, Camila P., Barranco, Isabel, Yeste Oliveras, Marc, Miró, Jordi
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/21271
Acceso en línea:http://hdl.handle.net/10256/21271
Access Level:acceso abierto
Palabra clave:Semen -- Crioconservació
Semen -- Cryopreservation
Cavalls -- Espermatozoides -- Investigació
Horses -- Spermatozoa -- Research
Antioxidants
id ES_fd331adef102e4c4643a6993ac47ebf1
oai_identifier_str oai:recercat.cat:10256/21271
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Seminal plasma antioxidants are related to sperm cryotolerance in the horse
title Seminal plasma antioxidants are related to sperm cryotolerance in the horse
spellingShingle Seminal plasma antioxidants are related to sperm cryotolerance in the horse
Catalán, Jaime
Semen -- Crioconservació
Semen -- Cryopreservation
Cavalls -- Espermatozoides -- Investigació
Horses -- Spermatozoa -- Research
Antioxidants
title_short Seminal plasma antioxidants are related to sperm cryotolerance in the horse
title_full Seminal plasma antioxidants are related to sperm cryotolerance in the horse
title_fullStr Seminal plasma antioxidants are related to sperm cryotolerance in the horse
title_full_unstemmed Seminal plasma antioxidants are related to sperm cryotolerance in the horse
title_sort Seminal plasma antioxidants are related to sperm cryotolerance in the horse
dc.creator.none.fl_str_mv Catalán, Jaime
Yáñez-Ortiz, Iván
Tvarijonaviciute, Asta
González-Arostegui, Luis Guillermo
Rubio, Camila P.
Barranco, Isabel
Yeste Oliveras, Marc
Miró, Jordi
author Catalán, Jaime
author_facet Catalán, Jaime
Yáñez-Ortiz, Iván
Tvarijonaviciute, Asta
González-Arostegui, Luis Guillermo
Rubio, Camila P.
Barranco, Isabel
Yeste Oliveras, Marc
Miró, Jordi
author_role author
author2 Yáñez-Ortiz, Iván
Tvarijonaviciute, Asta
González-Arostegui, Luis Guillermo
Rubio, Camila P.
Barranco, Isabel
Yeste Oliveras, Marc
Miró, Jordi
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación
dc.subject.none.fl_str_mv Semen -- Crioconservació
Semen -- Cryopreservation
Cavalls -- Espermatozoides -- Investigació
Horses -- Spermatozoa -- Research
Antioxidants
topic Semen -- Crioconservació
Semen -- Cryopreservation
Cavalls -- Espermatozoides -- Investigació
Horses -- Spermatozoa -- Research
Antioxidants
description The objective of this study was to determine the relationship of enzymatic (superoxide dismutase, SOD; glutathione peroxidase, GPX; catalase, CAT; and paraoxonase type 1, PON1) and non-enzymatic antioxidants (measured in terms of: Trolox equivalent antioxidant capacity, TEAC; cupric-reducing antioxidant capacity, CUPRAC; and ferric-reducing ability of plasma, FRAP), as well as the oxidative stress index (OSI) in seminal plasma (SP) with the resilience of horse sperm to freeze-thawing. Twenty-one ejaculates (one per individual) were collected and split into two aliquots: the first was used to harvest the SP and assess the activity levels of antioxidants and the OSI, and the second one was cryopreserved. The following post-thaw sperm quality parameters were evaluated: sperm motility, plasma membrane and acrosome integrity, mitochondrial membrane potential, intracellular levels of reactive oxygen species (ROS), and plasma membrane lipid disorder. Based on post-thaw total motility (TM) and plasma membrane integrity (SYBR14+/PI−), ejaculates were hierarchically (p < 0.001) clustered into two groups of good (GFE) and poor (PFE) freezability. The SP activity levels of PON1, SOD, and TEAC were higher (p < 0.05) in GFE than in PFE, showing a positive relationship (p < 0.05) with some sperm motility parameters and with plasma membrane (PON1 and TEAC) and acrosome (SOD and TEAC) integrity. In contrast, OSI was higher (p < 0.05) in the SP of PFE than in that of GFE, and was negatively correlated (p < 0.05) to some sperm motility parameters and to plasma membrane and acrosome integrity, and positively (p < 0.05) to the percentage of viable sperm with high plasma membrane lipid disorder. In conclusion, enzymatic (PON1 and SOD) and non-enzymatic (TEAC) antioxidants of SP are related to horse sperm cryotolerance. In addition, our results suggest that PON1 could be one of the main antioxidant enzymes involved in the control of ROS in this species. Further investigation is needed to confirm the potential use of these SP-antioxidants and OSI to predict sperm cryotolerance in horses
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer-reviewed
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/21271
http://hdl.handle.net/10256/21271
url http://hdl.handle.net/10256/21271
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/antiox11071279
info:eu-repo/semantics/altIdentifier/issn/2076-3921
info:eu-repo/semantics/altIdentifier/eissn/2076-3921
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113320RB-I00
dc.rights.none.fl_str_mv Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI (Multidisciplinary Digital Publishing Institute)
publisher.none.fl_str_mv MDPI (Multidisciplinary Digital Publishing Institute)
dc.source.none.fl_str_mv Antioxidants, 2022, vol. 11, núm. 7, p. 1279
Articles publicats (D-B)
Catalán, Jaime Yáñez-Ortiz, Iván Tvarijonaviciute, Asta González-Arostegui, Luis Guillermo Rubio, Camila P. Barranco, Isabel Yeste Oliveras, Marc Miró, Jordi 2022 Seminal plasma antioxidants are related to sperm cryotolerance in the horse Antioxidants 11 7 1279
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869425518270480384
spelling Seminal plasma antioxidants are related to sperm cryotolerance in the horseCatalán, JaimeYáñez-Ortiz, IvánTvarijonaviciute, AstaGonzález-Arostegui, Luis GuillermoRubio, Camila P.Barranco, IsabelYeste Oliveras, MarcMiró, JordiSemen -- CrioconservacióSemen -- CryopreservationCavalls -- Espermatozoides -- InvestigacióHorses -- Spermatozoa -- ResearchAntioxidantsThe objective of this study was to determine the relationship of enzymatic (superoxide dismutase, SOD; glutathione peroxidase, GPX; catalase, CAT; and paraoxonase type 1, PON1) and non-enzymatic antioxidants (measured in terms of: Trolox equivalent antioxidant capacity, TEAC; cupric-reducing antioxidant capacity, CUPRAC; and ferric-reducing ability of plasma, FRAP), as well as the oxidative stress index (OSI) in seminal plasma (SP) with the resilience of horse sperm to freeze-thawing. Twenty-one ejaculates (one per individual) were collected and split into two aliquots: the first was used to harvest the SP and assess the activity levels of antioxidants and the OSI, and the second one was cryopreserved. The following post-thaw sperm quality parameters were evaluated: sperm motility, plasma membrane and acrosome integrity, mitochondrial membrane potential, intracellular levels of reactive oxygen species (ROS), and plasma membrane lipid disorder. Based on post-thaw total motility (TM) and plasma membrane integrity (SYBR14+/PI−), ejaculates were hierarchically (p < 0.001) clustered into two groups of good (GFE) and poor (PFE) freezability. The SP activity levels of PON1, SOD, and TEAC were higher (p < 0.05) in GFE than in PFE, showing a positive relationship (p < 0.05) with some sperm motility parameters and with plasma membrane (PON1 and TEAC) and acrosome (SOD and TEAC) integrity. In contrast, OSI was higher (p < 0.05) in the SP of PFE than in that of GFE, and was negatively correlated (p < 0.05) to some sperm motility parameters and to plasma membrane and acrosome integrity, and positively (p < 0.05) to the percentage of viable sperm with high plasma membrane lipid disorder. In conclusion, enzymatic (PON1 and SOD) and non-enzymatic (TEAC) antioxidants of SP are related to horse sperm cryotolerance. In addition, our results suggest that PON1 could be one of the main antioxidant enzymes involved in the control of ROS in this species. Further investigation is needed to confirm the potential use of these SP-antioxidants and OSI to predict sperm cryotolerance in horsesJ.C. was funded by the project “Demetra” (Dipartamente di Eccellenza 2018–2022, Grant: CUP_C46C18000530001), Ministry for Education, University and Research, Italy. I.Y.-O. was funded by the Secretary of Higher Education, Science, Technology and Innovation (SENESCYT), Ecuador (Scheme: Programa de Becas Internacionales de Posgrado 2019; Grant: CZ02-000507-2019). I.B. was financially supported by the European Union’s Horizon 2020 research and innovation scheme (Grant: H2020-MSCA-IF-2019-891382). L.G-A. was funded by Fundación Séneca, Región de Murcia, Spain (Grant: 21453/FPI/20). C.P-R. was supported by the Ministry of Science and Innovation, Spain (Grant: FJC2019-042475-I). The authors also acknowledge the support from the Ministry of Science and Innovation, Spain (Grants: AGL2017-88329-R and PID2020-113320RB-I00), the Catalan Agency for Management of University and Research Grants, Regional Government of Catalonia, Spain (Grant: 2017-SGR-1229), and the Catalan Institution for Research and Advanced Studies (ICREA)MDPI (Multidisciplinary Digital Publishing Institute)Agencia Estatal de Investigación2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer-reviewedapplication/pdfhttp://hdl.handle.net/10256/21271http://hdl.handle.net/10256/21271Antioxidants, 2022, vol. 11, núm. 7, p. 1279Articles publicats (D-B)Catalán, Jaime Yáñez-Ortiz, Iván Tvarijonaviciute, Asta González-Arostegui, Luis Guillermo Rubio, Camila P. Barranco, Isabel Yeste Oliveras, Marc Miró, Jordi 2022 Seminal plasma antioxidants are related to sperm cryotolerance in the horse Antioxidants 11 7 1279reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.3390/antiox11071279info:eu-repo/semantics/altIdentifier/issn/2076-3921info:eu-repo/semantics/altIdentifier/eissn/2076-3921info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113320RB-I00Attribution 4.0 International (CC BY 4.0)https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10256/212712026-05-29T05:05:01Z
score 15.812429

Similares en LA Referencia