Non-invasive and simple methods for sampling rabbit DNA for PCR analysis of melanocortin 1 receptor (MC1R) gene mutations: a technical note
[EN] Simple and non-invasive methods of collecting and preserving biological specimens for genetic analysis can be useful in reducing the discomfort of the involved animals and can be applicable for highthroughput studies that require a large number of biosamples. In order to have the possibility to...
| Autores: | , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2007 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/9480 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/9480 |
| Access Level: | acceso abierto |
| Palabra clave: | Rabbit Hair dna Buccal swabs Biological specimens Direct pcr Mc1r mutations |
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Non-invasive and simple methods for sampling rabbit DNA for PCR analysis of melanocortin 1 receptor (MC1R) gene mutations: a technical noteFontanesi, L.Tazzoli, M.Russo, V.RabbitHair dnaBuccal swabsBiological specimensDirect pcrMc1r mutations[EN] Simple and non-invasive methods of collecting and preserving biological specimens for genetic analysis can be useful in reducing the discomfort of the involved animals and can be applicable for highthroughput studies that require a large number of biosamples. In order to have the possibility to analyse a large number of samples and making easier the collection of rabbit biological materials as well as to reduce the cost and time of the genotyping procedures, here we tested different protocols for the genotyping of mutations identified in the rabbit melanocortin receptor 1 (MC1R) gene that are associated to coat colours in different rabbit breeds. These protocols make use of rabbit hair roots or cell preparations from buccal swabs with a direct PCR amplification without previous DNA extraction. Hair samples were collected from 60 rabbits of different breeds and preserved for up to 12 months at 4°C. For each hair sample 5-10 hair roots were clipped a few mm from the root directly within a 0.2 ml PCR tube. Buccal cells were collected from 10 crossbred and purebred rabbits and prepared after a few steps for PCR. The PCR fragments were analysed by means of polyacrylamide gel electrophoresis and stained with ethidium bromide or using fluorescent labelled products resolved in a capillary automatic sequencer. The success genotyping rate of the hair and buccal cell samples was 98% and 80%, respectively. These values are comparable to the success rates of other protocols described in human or other animals involving previous DNA extraction. The obtained results show that noninvasive methods of biological specimen collection that make it possible to analyse the DNA without previous extraction can be easily and routinely applied in rabbit molecular genetic studies. However, due to the higher success rate and the easier method of collection, plucked hairs seem the best source for high-throughput biosampling in this species.This work was supported by RFO funds from the University of Bologna. We thank several rabbit breeders and Associazione Nazionale Coniglicoltori Italiani (ANCI) for their collaboration in the sampling of biological materialsWorld Rabbit Science. ICTA. UPVUniversità di BolognaRepositorio Institucional de la Universitat Politècnica de València Riunet20072007-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/9480reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/94802026-06-13T07:49:27Z |
| dc.title.none.fl_str_mv |
Non-invasive and simple methods for sampling rabbit DNA for PCR analysis of melanocortin 1 receptor (MC1R) gene mutations: a technical note |
| title |
Non-invasive and simple methods for sampling rabbit DNA for PCR analysis of melanocortin 1 receptor (MC1R) gene mutations: a technical note |
| spellingShingle |
Non-invasive and simple methods for sampling rabbit DNA for PCR analysis of melanocortin 1 receptor (MC1R) gene mutations: a technical note Fontanesi, L. Rabbit Hair dna Buccal swabs Biological specimens Direct pcr Mc1r mutations |
| title_short |
Non-invasive and simple methods for sampling rabbit DNA for PCR analysis of melanocortin 1 receptor (MC1R) gene mutations: a technical note |
| title_full |
Non-invasive and simple methods for sampling rabbit DNA for PCR analysis of melanocortin 1 receptor (MC1R) gene mutations: a technical note |
| title_fullStr |
Non-invasive and simple methods for sampling rabbit DNA for PCR analysis of melanocortin 1 receptor (MC1R) gene mutations: a technical note |
| title_full_unstemmed |
Non-invasive and simple methods for sampling rabbit DNA for PCR analysis of melanocortin 1 receptor (MC1R) gene mutations: a technical note |
| title_sort |
Non-invasive and simple methods for sampling rabbit DNA for PCR analysis of melanocortin 1 receptor (MC1R) gene mutations: a technical note |
| dc.creator.none.fl_str_mv |
Fontanesi, L. Tazzoli, M. Russo, V. |
| author |
Fontanesi, L. |
| author_facet |
Fontanesi, L. Tazzoli, M. Russo, V. |
| author_role |
author |
| author2 |
Tazzoli, M. Russo, V. |
| author2_role |
author author |
| dc.contributor.none.fl_str_mv |
Università di Bologna Repositorio Institucional de la Universitat Politècnica de València Riunet |
| dc.subject.none.fl_str_mv |
Rabbit Hair dna Buccal swabs Biological specimens Direct pcr Mc1r mutations |
| topic |
Rabbit Hair dna Buccal swabs Biological specimens Direct pcr Mc1r mutations |
| description |
[EN] Simple and non-invasive methods of collecting and preserving biological specimens for genetic analysis can be useful in reducing the discomfort of the involved animals and can be applicable for highthroughput studies that require a large number of biosamples. In order to have the possibility to analyse a large number of samples and making easier the collection of rabbit biological materials as well as to reduce the cost and time of the genotyping procedures, here we tested different protocols for the genotyping of mutations identified in the rabbit melanocortin receptor 1 (MC1R) gene that are associated to coat colours in different rabbit breeds. These protocols make use of rabbit hair roots or cell preparations from buccal swabs with a direct PCR amplification without previous DNA extraction. Hair samples were collected from 60 rabbits of different breeds and preserved for up to 12 months at 4°C. For each hair sample 5-10 hair roots were clipped a few mm from the root directly within a 0.2 ml PCR tube. Buccal cells were collected from 10 crossbred and purebred rabbits and prepared after a few steps for PCR. The PCR fragments were analysed by means of polyacrylamide gel electrophoresis and stained with ethidium bromide or using fluorescent labelled products resolved in a capillary automatic sequencer. The success genotyping rate of the hair and buccal cell samples was 98% and 80%, respectively. These values are comparable to the success rates of other protocols described in human or other animals involving previous DNA extraction. The obtained results show that noninvasive methods of biological specimen collection that make it possible to analyse the DNA without previous extraction can be easily and routinely applied in rabbit molecular genetic studies. However, due to the higher success rate and the easier method of collection, plucked hairs seem the best source for high-throughput biosampling in this species. |
| publishDate |
2007 |
| dc.date.none.fl_str_mv |
2007 2007-01-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://riunet.upv.es/handle/10251/9480 |
| url |
https://riunet.upv.es/handle/10251/9480 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reserva de todos los derechos http://rightsstatements.org/vocab/InC/1.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reserva de todos los derechos http://rightsstatements.org/vocab/InC/1.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
World Rabbit Science. ICTA. UPV |
| publisher.none.fl_str_mv |
World Rabbit Science. ICTA. UPV |
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reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname:Universitat Politècnica de València (UPV) |
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Universitat Politècnica de València (UPV) |
| reponame_str |
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| collection |
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
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