Cocoa intake attenuates renal injury in Zucker Diabetic fatty rats by improving glucose homeostasis

Glucotoxicity (high levels of glucose) is a major factor in the pathogenesis of diabetic kidney disease. Cocoa has anti-diabetic effects by lowering glucose levels. However, whether cocoa exerts beneficial effects on the renal cortex glucose homeostasis and the molecular mechanisms responsible for t...

Descripción completa

Detalles Bibliográficos
Autores: Álvarez-Cilleros, David, López-Oliva, Elvira, Goya, Luis, Martín, M. Ángeles, Ramos, Sonia
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/191569
Acceso en línea:http://hdl.handle.net/10261/191569
Access Level:acceso abierto
Palabra clave:Glucose toleranceInsulin resistance
Cocoa
Renal cortex structural alterations
Type 2 diabetic ZDF rats
Glucose homeostasis
id ES_fcf4b8c8a5faeb58ef96fe91f7114608
oai_identifier_str oai:digital.csic.es:10261/191569
network_acronym_str ES
network_name_str España
repository_id_str
spelling Cocoa intake attenuates renal injury in Zucker Diabetic fatty rats by improving glucose homeostasisÁlvarez-Cilleros, DavidLópez-Oliva, ElviraGoya, LuisMartín, M. ÁngelesRamos, SoniaGlucose toleranceInsulin resistanceCocoaRenal cortex structural alterationsType 2 diabetic ZDF ratsGlucose homeostasisGlucotoxicity (high levels of glucose) is a major factor in the pathogenesis of diabetic kidney disease. Cocoa has anti-diabetic effects by lowering glucose levels. However, whether cocoa exerts beneficial effects on the renal cortex glucose homeostasis and the molecular mechanisms responsible for this possible protective activity remain largely unknown. Thus, the potential anti-diabetic properties of cocoa on insulin signalling, glucose transporters and gluconeogenic enzymes were evaluated in the renal cortex of Zucker Diabetic fatty (ZDF) rats. Male ZDF rats were fed a control or cocoa-rich diet (10%), and Zucker Lean animals received the control diet. ZDF rats supplemented with cocoa (ZDF-Co) showed decreased body weight gain, glucose and insulin levels, improved glucose tolerance, insulin resistance and structural alterations in renal cortex. Moreover, cocoa-rich diet ameliorated insulin resistance by reverting decreased tyrosine-phosphorylated-insulin receptor levels and by preventing the inactivation of glycogen synthase kinase-3/glycogen synthase pathway (GSK-3/GS) in the renal cortex of ZDF-Co rats. Cocoa antihyperglycaemic effect also appeared to be mediated through the diminution of phosphoenolpyruvate-carboxykinase (PEPCK), glucose-6-phosphatase (G-6-Pase), sodium-glucose-co-transporter-2 (SGLT-2), and glucose-transporter-2 (GLUT-2) levels in ZDF-Co rat's renal cortex. These findings demonstrate that cocoa alleviates renal injury by contributing to maintain the glucose homeostasis in type 2 diabetic ZDF rats.This work was supported by the grant AGL2015-67087-R (MINECO/FEDER, UE) from the Spanish Ministry of Science and Innovation (MINECO). D. Álvarez-Cilleros is a FPI fellow from the predoctoral program of MINECO (BES-2016-076721).Peer ReviewedElsevierMinisterio de Economía y Competitividad (España)Ministerio de Ciencia e Innovación (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/191569reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-67087-Rhttps://doi.org/10.1016/j.fct.2019.03.002Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1915692026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cocoa intake attenuates renal injury in Zucker Diabetic fatty rats by improving glucose homeostasis
title Cocoa intake attenuates renal injury in Zucker Diabetic fatty rats by improving glucose homeostasis
spellingShingle Cocoa intake attenuates renal injury in Zucker Diabetic fatty rats by improving glucose homeostasis
Álvarez-Cilleros, David
Glucose toleranceInsulin resistance
Cocoa
Renal cortex structural alterations
Type 2 diabetic ZDF rats
Glucose homeostasis
title_short Cocoa intake attenuates renal injury in Zucker Diabetic fatty rats by improving glucose homeostasis
title_full Cocoa intake attenuates renal injury in Zucker Diabetic fatty rats by improving glucose homeostasis
title_fullStr Cocoa intake attenuates renal injury in Zucker Diabetic fatty rats by improving glucose homeostasis
title_full_unstemmed Cocoa intake attenuates renal injury in Zucker Diabetic fatty rats by improving glucose homeostasis
title_sort Cocoa intake attenuates renal injury in Zucker Diabetic fatty rats by improving glucose homeostasis
dc.creator.none.fl_str_mv Álvarez-Cilleros, David
López-Oliva, Elvira
Goya, Luis
Martín, M. Ángeles
Ramos, Sonia
author Álvarez-Cilleros, David
author_facet Álvarez-Cilleros, David
López-Oliva, Elvira
Goya, Luis
Martín, M. Ángeles
Ramos, Sonia
author_role author
author2 López-Oliva, Elvira
Goya, Luis
Martín, M. Ángeles
Ramos, Sonia
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia e Innovación (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Glucose toleranceInsulin resistance
Cocoa
Renal cortex structural alterations
Type 2 diabetic ZDF rats
Glucose homeostasis
topic Glucose toleranceInsulin resistance
Cocoa
Renal cortex structural alterations
Type 2 diabetic ZDF rats
Glucose homeostasis
description Glucotoxicity (high levels of glucose) is a major factor in the pathogenesis of diabetic kidney disease. Cocoa has anti-diabetic effects by lowering glucose levels. However, whether cocoa exerts beneficial effects on the renal cortex glucose homeostasis and the molecular mechanisms responsible for this possible protective activity remain largely unknown. Thus, the potential anti-diabetic properties of cocoa on insulin signalling, glucose transporters and gluconeogenic enzymes were evaluated in the renal cortex of Zucker Diabetic fatty (ZDF) rats. Male ZDF rats were fed a control or cocoa-rich diet (10%), and Zucker Lean animals received the control diet. ZDF rats supplemented with cocoa (ZDF-Co) showed decreased body weight gain, glucose and insulin levels, improved glucose tolerance, insulin resistance and structural alterations in renal cortex. Moreover, cocoa-rich diet ameliorated insulin resistance by reverting decreased tyrosine-phosphorylated-insulin receptor levels and by preventing the inactivation of glycogen synthase kinase-3/glycogen synthase pathway (GSK-3/GS) in the renal cortex of ZDF-Co rats. Cocoa antihyperglycaemic effect also appeared to be mediated through the diminution of phosphoenolpyruvate-carboxykinase (PEPCK), glucose-6-phosphatase (G-6-Pase), sodium-glucose-co-transporter-2 (SGLT-2), and glucose-transporter-2 (GLUT-2) levels in ZDF-Co rat's renal cortex. These findings demonstrate that cocoa alleviates renal injury by contributing to maintain the glucose homeostasis in type 2 diabetic ZDF rats.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/191569
url http://hdl.handle.net/10261/191569
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-67087-R
https://doi.org/10.1016/j.fct.2019.03.002
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869425496761040896
score 15.81155

Similares en LA Referencia