Kainate receptors regulate synaptic integrity and plasticity by forming a complex with synaptic organizers in the cerebellum

Kainate (KA)-type glutamate receptors (KARs) are implicated in various neuropsychiatric and neurological disorders through their ionotropic and metabotropic actions. However, compared to AMPA- and NMDA-type receptor functions, many aspects of KAR biology remain incompletely understood. Our study dem...

Descripción completa

Detalles Bibliográficos
Autores: Kakegawa, Wataru, Paternain, Ana V., Matsuda, Keiko, Aller, María Isabel, Iida, Izumi, Miura, Eriko, Nozawa, Kazuya, Yamasaki, Tokiwa, Sakimura, Kenji, Yuzaki, Michisuke, Lerma Gómez, Juan
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/380179
Acceso en línea:http://hdl.handle.net/10261/380179
https://api.elsevier.com/content/abstract/scopus_id/85198575550
Access Level:acceso abierto
Palabra clave:CP: Cell biology
CP: Neuroscience
Purkinje cell
Cerebellum
Kainate receptors
Motor learning
Synapse organizer
Synaptic plasticity
Descripción
Sumario:Kainate (KA)-type glutamate receptors (KARs) are implicated in various neuropsychiatric and neurological disorders through their ionotropic and metabotropic actions. However, compared to AMPA- and NMDA-type receptor functions, many aspects of KAR biology remain incompletely understood. Our study demonstrates an important role of KARs in organizing climbing fiber (CF)-Purkinje cell (PC) synapses and synaptic plasticity in the cerebellum, independently of their ion channel or metabotropic functions. The amino-terminal domain (ATD) of the GluK4 KAR subunit binds to C1ql1, provided by CFs, and associates with Bai3, an adhesion-type G protein-coupled receptor expressed in PC dendrites. Mice lacking GluK4 exhibit no KAR-mediated responses, reduced C1ql1 and Bai3 levels, and fewer CF-PC synapses, along with impaired long-term depression and oculomotor learning. Remarkably, introduction of the ATD of GluK4 significantly improves all these phenotypes. These findings demonstrate that KARs act as synaptic scaffolds, orchestrating synapses by forming a KAR-C1ql1-Bai3 complex in the cerebellum.