Polycaprolactone/gelatin-based scaffolds with tailored performance: in vitro and in vivo validation

[EN] Nanofibrous scaffolds composed of polycaprolactone (PCL) and gelatin (Ge) were obtained through a hydrolytic assisted electrospinning process. The PCL-to-Ge proportion (100/0 to 20/80), as well as the dissolution time (24, 48, 72, 96, 120 h) into a 1:1 formic/acetic acid solvent before electros...

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Autores: Gil-Castell, O., Badia, J.D., Ontoria-Oviedo, I., Castellano, D., Sepúlveda, P., Ribes-Greus, A.|||0000-0003-2460-8291
Formato: artículo
Fecha de publicación:2020
País:España
Recursos:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/231543
Acesso em linha:https://riunet.upv.es/handle/10251/231543
Access Level:acceso abierto
Palavra-chave:Scaffold,Tailored,in vitro,in vivo,Biocompatibility
03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades
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oai_identifier_str oai:riunet.upv.es:10251/231543
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Polycaprolactone/gelatin-based scaffolds with tailored performance: in vitro and in vivo validation
title Polycaprolactone/gelatin-based scaffolds with tailored performance: in vitro and in vivo validation
spellingShingle Polycaprolactone/gelatin-based scaffolds with tailored performance: in vitro and in vivo validation
Gil-Castell, O.
Scaffold,Tailored,in vitro,in vivo,Biocompatibility
03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades
title_short Polycaprolactone/gelatin-based scaffolds with tailored performance: in vitro and in vivo validation
title_full Polycaprolactone/gelatin-based scaffolds with tailored performance: in vitro and in vivo validation
title_fullStr Polycaprolactone/gelatin-based scaffolds with tailored performance: in vitro and in vivo validation
title_full_unstemmed Polycaprolactone/gelatin-based scaffolds with tailored performance: in vitro and in vivo validation
title_sort Polycaprolactone/gelatin-based scaffolds with tailored performance: in vitro and in vivo validation
dc.creator.none.fl_str_mv Gil-Castell, O.
Badia, J.D.
Ontoria-Oviedo, I.
Castellano, D.
Sepúlveda, P.
Ribes-Greus, A.|||0000-0003-2460-8291
author Gil-Castell, O.
author_facet Gil-Castell, O.
Badia, J.D.
Ontoria-Oviedo, I.
Castellano, D.
Sepúlveda, P.
Ribes-Greus, A.|||0000-0003-2460-8291
author_role author
author2 Badia, J.D.
Ontoria-Oviedo, I.
Castellano, D.
Sepúlveda, P.
Ribes-Greus, A.|||0000-0003-2460-8291
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Máquinas y Motores Térmicos
Escuela Técnica Superior de Ingeniería Industrial
 Instituto Universitario de Investigación de Tecnología de los Materiales de la UPV
Generalitat Valenciana
Ministerio de Educación
Instituto de Salud Carlos III
European Regional Development Fund
Ministerio de Economía y Competitividad
Ministerio de Educación, Cultura y Deporte
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Scaffold,Tailored,in vitro,in vivo,Biocompatibility
03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades
topic Scaffold,Tailored,in vitro,in vivo,Biocompatibility
03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades
description [EN] Nanofibrous scaffolds composed of polycaprolactone (PCL) and gelatin (Ge) were obtained through a hydrolytic assisted electrospinning process. The PCL-to-Ge proportion (100/0 to 20/80), as well as the dissolution time (24, 48, 72, 96, 120 h) into a 1:1 formic/acetic acid solvent before electrospinning were modified to obtain the different samples. A strong influence of these factors on the physicochemical properties of the scaffolds was observed. Higher Ge percentage reduced crystallinity, allowed a uniform morphology and increased water contact angle. The increase in the dissolution time considerably reduced the molar mass and, subsequently, fibre diameter and crystallinity were affected. During in vitro biocompatibility tests, higher cell adhesion and proliferation were found for the 60/40, 50/50 and 40/60 PCL/Ge compositions that was corroborated by MTT assay, fluorescence and microscopy. A weakened structure, more labile to the in vitro degradation in physiologic conditions was found for these compositions with higher dissolution times (72 and 96 h). Particularly, the 40/60 PCL/Ge scaffolds revealed an interesting progressive degradation behaviour as a function of the dissolution time. Moreover, these scaffolds were non-inflammatory, as revealed by the pyrogen test and after the 15-day subcutaneous in vivo implantation in mice. Finally, a reduction of the scar tissue area after infarction was found for the 40/60 PCL/Ge scaffolds electrospun after 72 h implanted in rat hearts. These results are especially interesting and represent a feasible way to avoid undesired inflammatory reactions during the scaffold assimilation.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-02-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/231543
url https://riunet.upv.es/handle/10251/231543
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv MINISTERIO DE EDUCACION MINISTERIO DE EDUCACION AP2013-01916 AYUDA CONTRATO FPU 2013-GIL CASTELL
Generalitat Valenciana https://doi.org/10.13039/501100003359 APOSTD%2F2014%2F041 AYUDA VALI+D POSTDOCTORAL-BADIA VALIENTE, JOSE DAVID
Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI16%2F0107
Ministerio de Educación y Cultura http://dx.doi.org/10.13039/501100003176 FPU13%2F01916 FPU13
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 RTC-2015-4185-1 INVESTIGACIÓN EN EL FRACCIONAMIENTO DE ACEITES ENRIQUECIDOS CON PRECURSORES DE MEDIADORES LIPIDICOS CON ACTIVIDAD ANTI-INFLAMATORIA Y PRO-RESOLUTIVA. APLICACIÓN AL DISEÑO Y DESARROLLO DE APÓSITOS PARA TRATAMIENTO DE CICATRIZACIÓN DE HERIDAS CRÓNICAS
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 RD16%2F0011%2F0004 Red de terapia celular (TERCEL)
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Polycaprolactone/gelatin-based scaffolds with tailored performance: in vitro and in vivo validationGil-Castell, O.Badia, J.D.Ontoria-Oviedo, I.Castellano, D.Sepúlveda, P.Ribes-Greus, A.|||0000-0003-2460-8291Scaffold,Tailored,in vitro,in vivo,Biocompatibility03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades[EN] Nanofibrous scaffolds composed of polycaprolactone (PCL) and gelatin (Ge) were obtained through a hydrolytic assisted electrospinning process. The PCL-to-Ge proportion (100/0 to 20/80), as well as the dissolution time (24, 48, 72, 96, 120 h) into a 1:1 formic/acetic acid solvent before electrospinning were modified to obtain the different samples. A strong influence of these factors on the physicochemical properties of the scaffolds was observed. Higher Ge percentage reduced crystallinity, allowed a uniform morphology and increased water contact angle. The increase in the dissolution time considerably reduced the molar mass and, subsequently, fibre diameter and crystallinity were affected. During in vitro biocompatibility tests, higher cell adhesion and proliferation were found for the 60/40, 50/50 and 40/60 PCL/Ge compositions that was corroborated by MTT assay, fluorescence and microscopy. A weakened structure, more labile to the in vitro degradation in physiologic conditions was found for these compositions with higher dissolution times (72 and 96 h). Particularly, the 40/60 PCL/Ge scaffolds revealed an interesting progressive degradation behaviour as a function of the dissolution time. Moreover, these scaffolds were non-inflammatory, as revealed by the pyrogen test and after the 15-day subcutaneous in vivo implantation in mice. Finally, a reduction of the scar tissue area after infarction was found for the 40/60 PCL/Ge scaffolds electrospun after 72 h implanted in rat hearts. These results are especially interesting and represent a feasible way to avoid undesired inflammatory reactions during the scaffold assimilation.The Spanish Ministry of Economy and Competitiveness is acknowledged for the project RESOLTEX project (RTC-2015-4185-1). The Spanish Ministry of Education, Culture and Sports is thanked for the pre-doctoral FPU grant of O. Gil-Castell (FPU13/01916). The Generalitat Valenciana is thanked for the APOSTD/2014/041 for J.D. Badia. The Universitat Politècnica de València and IIS LaFe are acknowledged for the SCARDIO project (2014_0460_CRC_COLAB_UPVLAFE_SEPULVEDA). P. Sepúlveda acknowledges the Instituto de Salud Carlos III (ISCIII) for the I3SNS Program. This work was supported by grants PI16/0107 and RETICS Program (RD16/0011/0004) from ISCIII cofunded by the European Regional Development Fund-ERDF Una manera de hacer Europa . The City Council of Algemesí is acknowledged for the XIV Premi Cientificotècnic Ciutat d Algemesí award to O. Gil-Castell, for this work as the best young researcher of the Ribera District.ElsevierDepartamento de Máquinas y Motores TérmicosEscuela Técnica Superior de Ingeniería Industrial Instituto Universitario de Investigación de Tecnología de los Materiales de la UPVGeneralitat ValencianaMinisterio de EducaciónInstituto de Salud Carlos IIIEuropean Regional Development FundMinisterio de Economía y CompetitividadMinisterio de Educación, Cultura y DeporteRepositorio Institucional de la Universitat Politècnica de València Riunet20202020-02-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/231543reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMINISTERIO DE EDUCACION MINISTERIO DE EDUCACION AP2013-01916 AYUDA CONTRATO FPU 2013-GIL CASTELLGeneralitat Valenciana https://doi.org/10.13039/501100003359 APOSTD%2F2014%2F041 AYUDA VALI+D POSTDOCTORAL-BADIA VALIENTE, JOSE DAVIDInstituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI16%2F0107Ministerio de Educación y Cultura http://dx.doi.org/10.13039/501100003176 FPU13%2F01916 FPU13Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 RTC-2015-4185-1 INVESTIGACIÓN EN EL FRACCIONAMIENTO DE ACEITES ENRIQUECIDOS CON PRECURSORES DE MEDIADORES LIPIDICOS CON ACTIVIDAD ANTI-INFLAMATORIA Y PRO-RESOLUTIVA. APLICACIÓN AL DISEÑO Y DESARROLLO DE APÓSITOS PARA TRATAMIENTO DE CICATRIZACIÓN DE HERIDAS CRÓNICASMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 RD16%2F0011%2F0004 Red de terapia celular (TERCEL)open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/2315432026-06-13T07:49:27Z
score 15,811543

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