Biomolecular characterization of Trichomonas vaginalis from Spain: evaluating genetic correlation with drug resistance and endobionts.

Trichomonas vaginalis causes one of the most prevalent sexually transmitted infections (STIs). Despite extensive biomolecular research on this protozoan, no efficient molecular tool currently exists for the intraspecific classification of T. vaginalis isolates. In recent years, an incipient tendency...

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Detalhes bibliográficos
Autores: Ibáñez Escribano, Alexandra, Nogal Ruiz, Juan José, Escario García-Trevijano, José Antonio, Ponce Gordo, Francisco
Formato: artículo
Fecha de publicación:2025
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/122264
Acesso em linha:https://hdl.handle.net/20.500.14352/122264
Access Level:acceso abierto
Palavra-chave:579.66
615.28
576.8
Trichomonas vaginalis
genotypes
TVV
Mycoplasma
resistance
microsatellites
single-copy gene
SNP
Microbiología (Farmacia)
Genética médica
Toxicología (Farmacia)
Parasitología (Farmacia)
3207.12 Parasitología
id ES_fce2c6491bcf3d34eaa8b17ac994fdba
oai_identifier_str oai:docta.ucm.es:20.500.14352/122264
network_acronym_str ES
network_name_str España
repository_id_str
spelling Biomolecular characterization of Trichomonas vaginalis from Spain: evaluating genetic correlation with drug resistance and endobionts.Ibáñez Escribano, AlexandraNogal Ruiz, Juan JoséEscario García-Trevijano, José AntonioPonce Gordo, Francisco579.66615.28576.8Trichomonas vaginalisgenotypesTVVMycoplasmaresistancemicrosatellitessingle-copy geneSNPMicrobiología (Farmacia)Genética médicaToxicología (Farmacia)Parasitología (Farmacia)3207.12 ParasitologíaTrichomonas vaginalis causes one of the most prevalent sexually transmitted infections (STIs). Despite extensive biomolecular research on this protozoan, no efficient molecular tool currently exists for the intraspecific classification of T. vaginalis isolates. In recent years, an incipient tendency has been observed to classify this parasite into two genotypes that correlate in a high percentage with phenotypic characteristics (such as metronidazole resistance and viral endobionts). However, this grouping method has not yet been consolidated by the scientific community. In this sense, Mycoplasma hominis has also been identified as an endobiont of T. vaginalis, but no previous studies on its presence and possible correlation with the two T. vaginalis populations detected have been executed. In the present study, several new T. vaginalis isolates of Spanish origin have been characterized using two molecular markers (microsatellites and single-copy genes), the presence of T. vaginalis virus (TVV) and Mycoplasma, and the resistance to reference drugs. On the basis of our molecular results, the isolates were classified into two groups, as proposed by other researchers. In relation to the biological determinations, the two isolates harboring Mycoplasma were sensible to the reference drugs and were included in the same genotypic group. Although the presence of TVV was more notable in one group than in the other, both groups had TTV+ and TVV− isolates. These findings indicate that genetic grouping does not strongly correlate with susceptibility to reference drugs or endobiont presence, suggesting the need for further research into alternative classification models.MDPIUniversidad Complutense de Madrid20252025-01-0120252025-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/122264reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1222642026-06-02T12:44:21Z
dc.title.none.fl_str_mv Biomolecular characterization of Trichomonas vaginalis from Spain: evaluating genetic correlation with drug resistance and endobionts.
title Biomolecular characterization of Trichomonas vaginalis from Spain: evaluating genetic correlation with drug resistance and endobionts.
spellingShingle Biomolecular characterization of Trichomonas vaginalis from Spain: evaluating genetic correlation with drug resistance and endobionts.
Ibáñez Escribano, Alexandra
579.66
615.28
576.8
Trichomonas vaginalis
genotypes
TVV
Mycoplasma
resistance
microsatellites
single-copy gene
SNP
Microbiología (Farmacia)
Genética médica
Toxicología (Farmacia)
Parasitología (Farmacia)
3207.12 Parasitología
title_short Biomolecular characterization of Trichomonas vaginalis from Spain: evaluating genetic correlation with drug resistance and endobionts.
title_full Biomolecular characterization of Trichomonas vaginalis from Spain: evaluating genetic correlation with drug resistance and endobionts.
title_fullStr Biomolecular characterization of Trichomonas vaginalis from Spain: evaluating genetic correlation with drug resistance and endobionts.
title_full_unstemmed Biomolecular characterization of Trichomonas vaginalis from Spain: evaluating genetic correlation with drug resistance and endobionts.
title_sort Biomolecular characterization of Trichomonas vaginalis from Spain: evaluating genetic correlation with drug resistance and endobionts.
dc.creator.none.fl_str_mv Ibáñez Escribano, Alexandra
Nogal Ruiz, Juan José
Escario García-Trevijano, José Antonio
Ponce Gordo, Francisco
author Ibáñez Escribano, Alexandra
author_facet Ibáñez Escribano, Alexandra
Nogal Ruiz, Juan José
Escario García-Trevijano, José Antonio
Ponce Gordo, Francisco
author_role author
author2 Nogal Ruiz, Juan José
Escario García-Trevijano, José Antonio
Ponce Gordo, Francisco
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 579.66
615.28
576.8
Trichomonas vaginalis
genotypes
TVV
Mycoplasma
resistance
microsatellites
single-copy gene
SNP
Microbiología (Farmacia)
Genética médica
Toxicología (Farmacia)
Parasitología (Farmacia)
3207.12 Parasitología
topic 579.66
615.28
576.8
Trichomonas vaginalis
genotypes
TVV
Mycoplasma
resistance
microsatellites
single-copy gene
SNP
Microbiología (Farmacia)
Genética médica
Toxicología (Farmacia)
Parasitología (Farmacia)
3207.12 Parasitología
description Trichomonas vaginalis causes one of the most prevalent sexually transmitted infections (STIs). Despite extensive biomolecular research on this protozoan, no efficient molecular tool currently exists for the intraspecific classification of T. vaginalis isolates. In recent years, an incipient tendency has been observed to classify this parasite into two genotypes that correlate in a high percentage with phenotypic characteristics (such as metronidazole resistance and viral endobionts). However, this grouping method has not yet been consolidated by the scientific community. In this sense, Mycoplasma hominis has also been identified as an endobiont of T. vaginalis, but no previous studies on its presence and possible correlation with the two T. vaginalis populations detected have been executed. In the present study, several new T. vaginalis isolates of Spanish origin have been characterized using two molecular markers (microsatellites and single-copy genes), the presence of T. vaginalis virus (TVV) and Mycoplasma, and the resistance to reference drugs. On the basis of our molecular results, the isolates were classified into two groups, as proposed by other researchers. In relation to the biological determinations, the two isolates harboring Mycoplasma were sensible to the reference drugs and were included in the same genotypic group. Although the presence of TVV was more notable in one group than in the other, both groups had TTV+ and TVV− isolates. These findings indicate that genetic grouping does not strongly correlate with susceptibility to reference drugs or endobiont presence, suggesting the need for further research into alternative classification models.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-01-01
2025
2025-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/122264
url https://hdl.handle.net/20.500.14352/122264
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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