Swept-sine noise-induced damage as a hearing loss model for preclinical assays.

Mouse models are key tools for studying cochlear alterations in noise-induced hearing loss (NIHL) and for evaluating new therapies. Stimuli used to induce deafness in mice are usually white and octave band noises that include very low frequencies, considering the large mouse auditory range. We desig...

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Autores: Sanz López, Lorena, Murillo Cuesta, Silvia, Cobo, Pedro, Cediel Algovia, Rafael, Contreras, Julio, Rivera, Teresa, Varela Nieto, Isabel, Avendaño, Carlos
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Universidad Francisco de Vitoria
Repositorio:DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria
Idioma:inglés
OAI Identifier:oai:ddfv.ufv.es:10641/3922
Acceso en línea:https://hdl.handle.net/10641/3922
Access Level:acceso abierto
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spelling Swept-sine noise-induced damage as a hearing loss model for preclinical assays.Sanz López, LorenaMurillo Cuesta, SilviaCobo, PedroCediel Algovia, RafaelContreras, JulioRivera, TeresaVarela Nieto, IsabelAvendaño, CarlosMouse models are key tools for studying cochlear alterations in noise-induced hearing loss (NIHL) and for evaluating new therapies. Stimuli used to induce deafness in mice are usually white and octave band noises that include very low frequencies, considering the large mouse auditory range. We designed different sound stimuli, enriched in frequencies up to 20 kHz (“violet” noises) to examine their impact on hearing thresholds and cochlear cytoarchitecture after short exposure. In addition, we developed a cytocochleogram to quantitatively assess the ensuing structural degeneration and its functional correlation. Finally, we used this mouse model and cochleogram procedure to evaluate the potential therapeutic effect of transforming growth factor β1 (TGF-β1) inhibitors P17 and P144 on NIHL. CBA mice were exposed to violet swept-sine noise (VS) with different frequency ranges (2–20 or 9–13 kHz) and levels (105 or 120 dB SPL) for 30 min. Mice were evaluated by auditory brainstem response (ABR) and otoacoustic emission tests prior to and 2, 14 and 28 days after noise exposure. Cochlear pathology was assessed with gross histology; hair cell number was estimated by a stereological counting method. Our results indicate that functional and morphological changes induced by VS depend on the sound level and frequency composition. Partial hearing recovery followed the exposure to 105 dB SPL, whereas permanent cochlear damage resulted from the exposure to 120 dB SPL. Exposure to 9–13 kHz noise caused an auditory threshold shift (TS) in those frequencies that correlated with hair cell loss in the corresponding areas of the cochlea that were spotted on the cytocochleogram. In summary, we present mouse models of NIHL, which depending on the sound properties of the noise, cause different degrees of cochlear damage, and could therefore be used to study molecules which are potential players in hearing loss protection and repair.20152015-01-0120152015-01-01journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10641/3922reponame:DDFV. Repositorio Institucional de la Universidad Francisco de Vitoriainstname:Universidad Francisco de VitoriaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:ddfv.ufv.es:10641/39222026-06-11T12:44:57Z
dc.title.none.fl_str_mv Swept-sine noise-induced damage as a hearing loss model for preclinical assays.
title Swept-sine noise-induced damage as a hearing loss model for preclinical assays.
spellingShingle Swept-sine noise-induced damage as a hearing loss model for preclinical assays.
Sanz López, Lorena
title_short Swept-sine noise-induced damage as a hearing loss model for preclinical assays.
title_full Swept-sine noise-induced damage as a hearing loss model for preclinical assays.
title_fullStr Swept-sine noise-induced damage as a hearing loss model for preclinical assays.
title_full_unstemmed Swept-sine noise-induced damage as a hearing loss model for preclinical assays.
title_sort Swept-sine noise-induced damage as a hearing loss model for preclinical assays.
dc.creator.none.fl_str_mv Sanz López, Lorena
Murillo Cuesta, Silvia
Cobo, Pedro
Cediel Algovia, Rafael
Contreras, Julio
Rivera, Teresa
Varela Nieto, Isabel
Avendaño, Carlos
author Sanz López, Lorena
author_facet Sanz López, Lorena
Murillo Cuesta, Silvia
Cobo, Pedro
Cediel Algovia, Rafael
Contreras, Julio
Rivera, Teresa
Varela Nieto, Isabel
Avendaño, Carlos
author_role author
author2 Murillo Cuesta, Silvia
Cobo, Pedro
Cediel Algovia, Rafael
Contreras, Julio
Rivera, Teresa
Varela Nieto, Isabel
Avendaño, Carlos
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
description Mouse models are key tools for studying cochlear alterations in noise-induced hearing loss (NIHL) and for evaluating new therapies. Stimuli used to induce deafness in mice are usually white and octave band noises that include very low frequencies, considering the large mouse auditory range. We designed different sound stimuli, enriched in frequencies up to 20 kHz (“violet” noises) to examine their impact on hearing thresholds and cochlear cytoarchitecture after short exposure. In addition, we developed a cytocochleogram to quantitatively assess the ensuing structural degeneration and its functional correlation. Finally, we used this mouse model and cochleogram procedure to evaluate the potential therapeutic effect of transforming growth factor β1 (TGF-β1) inhibitors P17 and P144 on NIHL. CBA mice were exposed to violet swept-sine noise (VS) with different frequency ranges (2–20 or 9–13 kHz) and levels (105 or 120 dB SPL) for 30 min. Mice were evaluated by auditory brainstem response (ABR) and otoacoustic emission tests prior to and 2, 14 and 28 days after noise exposure. Cochlear pathology was assessed with gross histology; hair cell number was estimated by a stereological counting method. Our results indicate that functional and morphological changes induced by VS depend on the sound level and frequency composition. Partial hearing recovery followed the exposure to 105 dB SPL, whereas permanent cochlear damage resulted from the exposure to 120 dB SPL. Exposure to 9–13 kHz noise caused an auditory threshold shift (TS) in those frequencies that correlated with hair cell loss in the corresponding areas of the cochlea that were spotted on the cytocochleogram. In summary, we present mouse models of NIHL, which depending on the sound properties of the noise, cause different degrees of cochlear damage, and could therefore be used to study molecules which are potential players in hearing loss protection and repair.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01
2015
2015-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10641/3922
url https://hdl.handle.net/10641/3922
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
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Atribución-NoComercial-SinDerivadas 3.0 España
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria
instname:Universidad Francisco de Vitoria
instname_str Universidad Francisco de Vitoria
reponame_str DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria
collection DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria
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