Interferons are pro-inflammatory cytokines in sheared-stressed human aortic valve endothelial cells

© 2021 by the authors.

Detalles Bibliográficos
Autores: Parra Izquierdo, Iván, Sánchez-Bayuela, Tania, López, Javier, Gómez, Cristina, Pérez-Riesgo, Enrique, San Román, José Alberto, Sánchez Crespo, Mariano, Yacoub, Magdi, Chester, Adrian H., García-Rodríguez, Carmen
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/260790
Acceso en línea:http://hdl.handle.net/10261/260790
Access Level:acceso abierto
Palabra clave:Valvular endothelial cells
Interferons
Monocyte adhesion
Inflammation
JAK/STAT
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spelling Interferons are pro-inflammatory cytokines in sheared-stressed human aortic valve endothelial cellsParra Izquierdo, IvánSánchez-Bayuela, TaniaLópez, JavierGómez, CristinaPérez-Riesgo, EnriqueSan Román, José AlbertoSánchez Crespo, MarianoYacoub, MagdiChester, Adrian H.García-Rodríguez, CarmenValvular endothelial cellsInterferonsMonocyte adhesionInflammationJAK/STAT© 2021 by the authors.Calcific aortic valve disease (CAVD) is an athero-inflammatory process. Growing evidence supports the inflammation-driven calcification model, mediated by cytokines such as interferons (IFNs) and tumor necrosis factor (TNF)-α. Our goal was investigating IFNs’ effects in human aortic valve endothelial cells (VEC) and the potential differences between aortic (aVEC) and ventricular (vVEC) side cells. The endothelial phenotype was analyzed by Western blot, qPCR, ELISA, monocyte adhesion, and migration assays. In mixed VEC populations, IFNs promoted the activation of signal transducers and activators of transcription-1 and nuclear factor-κB, and the subsequent up-regulation of pro-inflammatory molecules. Side-specific VEC were activated with IFN-γ and TNF-α in an orbital shaker flow system. TNF-α, but not IFN-γ, induced hypoxia-inducible factor (HIF)-1α stabilization or endothelial nitric oxide synthase downregulation. Additionally, IFN-γ inhibited TNF-α–induced migration of aVEC. Also, IFN-γ triggered cytokine secretion and adhesion molecule expression in aVEC and vVEC. Finally, aVEC were more prone to cytokine-mediated monocyte adhesion under multiaxial flow conditions as compared with uniaxial flow. In conclusion, IFNs promote inflammation and reduce TNF-α–mediated migration in human VEC. Moreover, monocyte adhesion was higher in inflamed aVEC sheared under multiaxial flow, which may be relevant to understanding the initial stages of CAVD.This research was funded by grants from Spanish Ministry of Economy and Competitiveness, co-funded by the European Social Fund FEDER (SAF2013-44521-R, SAF2017-83079-R, PID2020- 113751RB-I00); Junta de Castilla y León (BIO/VA47/14, BIO/VA36/15, CSI035P17, GRS1432/A/16, GRS2205/A/2020, VA175P20, and Programa Estratégico IBGM-Escalera de Excelencia Ref. CLU2019-02); Fundación Domingo Martínez; and the Instituto de Salud Carlos III (Spanish Ministry of Health PI14/00022, CIBERCV CB16/11/00260). I.P. and T.S.B., PhD fellows from Valladolid University-Banco de Santander. A.C. was funded by the Magdi Yacoub Institute.Multidisciplinary Digital Publishing InstituteMinisterio de Economía y Competitividad (España)Agencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Junta de Castilla y LeónCSIC-UVA - Instituto de Biología y Genética Molecular (IBGM)Fundación Domingo MartínezInstituto de Salud Carlos IIIMinisterio de Sanidad (España)Universidad de ValladolidBanco SantanderMagdi Yacoub InstituteConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220212022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/260790reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//SAF2013-44521-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2017-83079-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113751RB-I00http://dx.doi.org/10.3390/ijms221910605Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2607902026-05-22T06:33:51Z
dc.title.none.fl_str_mv Interferons are pro-inflammatory cytokines in sheared-stressed human aortic valve endothelial cells
title Interferons are pro-inflammatory cytokines in sheared-stressed human aortic valve endothelial cells
spellingShingle Interferons are pro-inflammatory cytokines in sheared-stressed human aortic valve endothelial cells
Parra Izquierdo, Iván
Valvular endothelial cells
Interferons
Monocyte adhesion
Inflammation
JAK/STAT
title_short Interferons are pro-inflammatory cytokines in sheared-stressed human aortic valve endothelial cells
title_full Interferons are pro-inflammatory cytokines in sheared-stressed human aortic valve endothelial cells
title_fullStr Interferons are pro-inflammatory cytokines in sheared-stressed human aortic valve endothelial cells
title_full_unstemmed Interferons are pro-inflammatory cytokines in sheared-stressed human aortic valve endothelial cells
title_sort Interferons are pro-inflammatory cytokines in sheared-stressed human aortic valve endothelial cells
dc.creator.none.fl_str_mv Parra Izquierdo, Iván
Sánchez-Bayuela, Tania
López, Javier
Gómez, Cristina
Pérez-Riesgo, Enrique
San Román, José Alberto
Sánchez Crespo, Mariano
Yacoub, Magdi
Chester, Adrian H.
García-Rodríguez, Carmen
author Parra Izquierdo, Iván
author_facet Parra Izquierdo, Iván
Sánchez-Bayuela, Tania
López, Javier
Gómez, Cristina
Pérez-Riesgo, Enrique
San Román, José Alberto
Sánchez Crespo, Mariano
Yacoub, Magdi
Chester, Adrian H.
García-Rodríguez, Carmen
author_role author
author2 Sánchez-Bayuela, Tania
López, Javier
Gómez, Cristina
Pérez-Riesgo, Enrique
San Román, José Alberto
Sánchez Crespo, Mariano
Yacoub, Magdi
Chester, Adrian H.
García-Rodríguez, Carmen
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Junta de Castilla y León
CSIC-UVA - Instituto de Biología y Genética Molecular (IBGM)
Fundación Domingo Martínez
Instituto de Salud Carlos III
Ministerio de Sanidad (España)
Universidad de Valladolid
Banco Santander
Magdi Yacoub Institute
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Valvular endothelial cells
Interferons
Monocyte adhesion
Inflammation
JAK/STAT
topic Valvular endothelial cells
Interferons
Monocyte adhesion
Inflammation
JAK/STAT
description © 2021 by the authors.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/260790
url http://hdl.handle.net/10261/260790
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO//SAF2013-44521-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2017-83079-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113751RB-I00
http://dx.doi.org/10.3390/ijms221910605
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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