Green production of cladribine by using immobilized 2'-deoxyribosyltransferase from Lactobacillus delbrueckii stabilized through a double covalent/entrapment Technology
Nowadays, enzyme-mediated processes offer an eco-friendly and efficient alternative to the traditional multistep and environmentally harmful chemical processes. Herein we report the enzymatic synthesis of cladribine by a novel 2′-deoxyribosyltransferase (NDT)-based combined biocatalyst. To this end,...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/109033 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/109033 |
| Access Level: | acceso abierto |
| Palabra clave: | 577.15 577.2 60 615.012 Biomimetic silica Enzyme immobilization Glutaraldehyde Entrapment Calcium alginate Antineoplastic drug Bioquímica (Biología) Biología molecular (Biología) Medicamentos Biotecnología 2403 Bioquímica 2415 Biología Molecular 2302.09 Enzimología 3209.08 Preparación de Medicamentos |
| Sumario: | Nowadays, enzyme-mediated processes offer an eco-friendly and efficient alternative to the traditional multistep and environmentally harmful chemical processes. Herein we report the enzymatic synthesis of cladribine by a novel 2′-deoxyribosyltransferase (NDT)-based combined biocatalyst. To this end, Lactobacillus delbrueckii NDT (LdNDT) was successfully immobilized through a two-step immobilization methodology, including a covalent immobilization onto glutaraldehyde-activated biomimetic silica nanoparticles followed by biocatalyst entrapment in calcium alginate. The resulting immobilized derivative, SiGPEI 25000-LdNDT-Alg, displayed 98% retained activity and was shown to be active and stable in a broad range of pH (5–9) and temperature (30–60 °C), but also displayed an extremely high reusability (up to 2100 reuses without negligible loss of activity) in the enzymatic production of cladribine. Finally, as a proof of concept, SiGPEI 25000-LdNDT-Alg was successfully employed in the green production of cladribine at mg scale. |
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