Id2 leaves the chromatin of the E2F4-p130-controlled c-myc promoter during hepatocyte priming for liver regeneration
The Id (inhibitor of DNA binding or inhibitor of differentiation) helix-loop-helix proteins are involved in the regulation of cell growth, differentiation and cancer. The fact that the molecular mechanisms of liver regeneration are not completely understood prompted us to study the fate of Id2 in pr...
| Autores: | , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2006 |
| País: | España |
| Institución: | Universidad de Navarra |
| Repositorio: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Idioma: | inglés |
| OAI Identifier: | oai:dadun.unav.edu:10171/23244 |
| Acceso en línea: | https://hdl.handle.net/10171/23244 |
| Access Level: | acceso abierto |
| Palabra clave: | Cell cycle C-myc E2F Histone deacetylase Id2 Liver regeneration |
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Id2 leaves the chromatin of the E2F4-p130-controlled c-myc promoter during hepatocyte priming for liver regenerationRodriguez, J.L. (José L.)|||/items/fa2733a5-c7c0-4e99-9d41-64ee4cf1c54fSandoval, J. (Juan)|||/items/15e9a801-000b-48f9-8cc5-6747af534849Serviddio, G. (Gaetano)|||/items/b725f3dc-afb5-4529-9f8a-7578ec11a8b8Sastre, J. (Juan)|||/items/4f60e4a7-486b-48bb-ace1-2d07ab159b91Morante, M. (María)|||/items/f86d8861-7b9e-405f-b0bb-47b4d652c2e1Perrelli, M.J. (María-Giulia)|||/items/3c418076-9fb9-4f8f-a51a-2bd1d8bcee12Martinez-Chantar, M.L. (María Luz)|||/items/df06120b-2a53-4074-a155-638f899b2ce5Viña, J. (José)|||/items/3a94588e-f488-4d62-99b2-c639dc571e72Mato, J.M. (José María)|||/items/302dc624-b0d3-4703-90cf-1a97690ebc79Avila, M.A. (Matías Antonio)|||/items/3ad9abbb-c18d-445b-86cf-cb76be15419fFranco, L. (Luis)|||/items/3ca4ede0-fdd2-4a83-a72f-b90cf9570816Viña, J.R. (Juan R.)|||/items/28989225-9c39-4495-ae54-ba5fbb8f3963Lopez-Rodas, G. (Gerardo)|||/items/32cdfe1e-70b1-48f3-8992-236a1d17bb83Torres, L. (Luis)|||/items/e4740a05-e8bb-4f09-b3ca-e119dae6742bCell cycleC-mycE2FHistone deacetylaseId2Liver regenerationThe Id (inhibitor of DNA binding or inhibitor of differentiation) helix-loop-helix proteins are involved in the regulation of cell growth, differentiation and cancer. The fact that the molecular mechanisms of liver regeneration are not completely understood prompted us to study the fate of Id2 in proliferating liver. Id2 increases in liver regeneration after partial hepatectomy, following the early induction of its gene. Co-immunoprecipitation shows that Id2 forms a complex with E2F4, p130 and mSin3A in quiescent liver and all these components are present at the c-myc promoter as shown using ChIP (chromatin immunoprecipitation). Activation of c-myc during hepatocyte priming (G0-G1 transition) correlates with the dissociation of Id2 and HDAC (histone deacetylase), albeit p130 remains bound at least until 6 h. Moreover, as the G0-G1 transition progresses, Id2 and HDAC again bind the c-myc promoter concomitantly with the repression of this gene. The time course of c-myc binding to the Id2 promoter, as determined by ChIP assays is compatible with a role of the oncoprotein as a transcriptional inducer of Id2 in liver regeneration. Immunohistochemical analysis shows that Id2 also increases in proliferating hepatocytes after bile duct ligation. In this case, the pattern of Id2 presence in the c-myc promoter parallels that found in regenerating liver. Our results may suggest a control role for Id2 in hepatocyte priming, through a p130 dissociation-independent regulation of c-mycPortland PressDadun. Depósito Académico Digital Universidad de Navarra20122012-09-2720062006-01-0120062006-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/23244reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/232442026-06-21T12:47:57Z |
| dc.title.none.fl_str_mv |
Id2 leaves the chromatin of the E2F4-p130-controlled c-myc promoter during hepatocyte priming for liver regeneration |
| title |
Id2 leaves the chromatin of the E2F4-p130-controlled c-myc promoter during hepatocyte priming for liver regeneration |
| spellingShingle |
Id2 leaves the chromatin of the E2F4-p130-controlled c-myc promoter during hepatocyte priming for liver regeneration Rodriguez, J.L. (José L.)|||/items/fa2733a5-c7c0-4e99-9d41-64ee4cf1c54f Cell cycle C-myc E2F Histone deacetylase Id2 Liver regeneration |
| title_short |
Id2 leaves the chromatin of the E2F4-p130-controlled c-myc promoter during hepatocyte priming for liver regeneration |
| title_full |
Id2 leaves the chromatin of the E2F4-p130-controlled c-myc promoter during hepatocyte priming for liver regeneration |
| title_fullStr |
Id2 leaves the chromatin of the E2F4-p130-controlled c-myc promoter during hepatocyte priming for liver regeneration |
| title_full_unstemmed |
Id2 leaves the chromatin of the E2F4-p130-controlled c-myc promoter during hepatocyte priming for liver regeneration |
| title_sort |
Id2 leaves the chromatin of the E2F4-p130-controlled c-myc promoter during hepatocyte priming for liver regeneration |
| dc.creator.none.fl_str_mv |
Rodriguez, J.L. (José L.)|||/items/fa2733a5-c7c0-4e99-9d41-64ee4cf1c54f Sandoval, J. (Juan)|||/items/15e9a801-000b-48f9-8cc5-6747af534849 Serviddio, G. (Gaetano)|||/items/b725f3dc-afb5-4529-9f8a-7578ec11a8b8 Sastre, J. (Juan)|||/items/4f60e4a7-486b-48bb-ace1-2d07ab159b91 Morante, M. (María)|||/items/f86d8861-7b9e-405f-b0bb-47b4d652c2e1 Perrelli, M.J. (María-Giulia)|||/items/3c418076-9fb9-4f8f-a51a-2bd1d8bcee12 Martinez-Chantar, M.L. (María Luz)|||/items/df06120b-2a53-4074-a155-638f899b2ce5 Viña, J. (José)|||/items/3a94588e-f488-4d62-99b2-c639dc571e72 Mato, J.M. (José María)|||/items/302dc624-b0d3-4703-90cf-1a97690ebc79 Avila, M.A. (Matías Antonio)|||/items/3ad9abbb-c18d-445b-86cf-cb76be15419f Franco, L. (Luis)|||/items/3ca4ede0-fdd2-4a83-a72f-b90cf9570816 Viña, J.R. (Juan R.)|||/items/28989225-9c39-4495-ae54-ba5fbb8f3963 Lopez-Rodas, G. (Gerardo)|||/items/32cdfe1e-70b1-48f3-8992-236a1d17bb83 Torres, L. (Luis)|||/items/e4740a05-e8bb-4f09-b3ca-e119dae6742b |
| author |
Rodriguez, J.L. (José L.)|||/items/fa2733a5-c7c0-4e99-9d41-64ee4cf1c54f |
| author_facet |
Rodriguez, J.L. (José L.)|||/items/fa2733a5-c7c0-4e99-9d41-64ee4cf1c54f Sandoval, J. (Juan)|||/items/15e9a801-000b-48f9-8cc5-6747af534849 Serviddio, G. (Gaetano)|||/items/b725f3dc-afb5-4529-9f8a-7578ec11a8b8 Sastre, J. (Juan)|||/items/4f60e4a7-486b-48bb-ace1-2d07ab159b91 Morante, M. (María)|||/items/f86d8861-7b9e-405f-b0bb-47b4d652c2e1 Perrelli, M.J. (María-Giulia)|||/items/3c418076-9fb9-4f8f-a51a-2bd1d8bcee12 Martinez-Chantar, M.L. (María Luz)|||/items/df06120b-2a53-4074-a155-638f899b2ce5 Viña, J. (José)|||/items/3a94588e-f488-4d62-99b2-c639dc571e72 Mato, J.M. (José María)|||/items/302dc624-b0d3-4703-90cf-1a97690ebc79 Avila, M.A. (Matías Antonio)|||/items/3ad9abbb-c18d-445b-86cf-cb76be15419f Franco, L. (Luis)|||/items/3ca4ede0-fdd2-4a83-a72f-b90cf9570816 Viña, J.R. (Juan R.)|||/items/28989225-9c39-4495-ae54-ba5fbb8f3963 Lopez-Rodas, G. (Gerardo)|||/items/32cdfe1e-70b1-48f3-8992-236a1d17bb83 Torres, L. (Luis)|||/items/e4740a05-e8bb-4f09-b3ca-e119dae6742b |
| author_role |
author |
| author2 |
Sandoval, J. (Juan)|||/items/15e9a801-000b-48f9-8cc5-6747af534849 Serviddio, G. (Gaetano)|||/items/b725f3dc-afb5-4529-9f8a-7578ec11a8b8 Sastre, J. (Juan)|||/items/4f60e4a7-486b-48bb-ace1-2d07ab159b91 Morante, M. (María)|||/items/f86d8861-7b9e-405f-b0bb-47b4d652c2e1 Perrelli, M.J. (María-Giulia)|||/items/3c418076-9fb9-4f8f-a51a-2bd1d8bcee12 Martinez-Chantar, M.L. (María Luz)|||/items/df06120b-2a53-4074-a155-638f899b2ce5 Viña, J. (José)|||/items/3a94588e-f488-4d62-99b2-c639dc571e72 Mato, J.M. (José María)|||/items/302dc624-b0d3-4703-90cf-1a97690ebc79 Avila, M.A. (Matías Antonio)|||/items/3ad9abbb-c18d-445b-86cf-cb76be15419f Franco, L. (Luis)|||/items/3ca4ede0-fdd2-4a83-a72f-b90cf9570816 Viña, J.R. (Juan R.)|||/items/28989225-9c39-4495-ae54-ba5fbb8f3963 Lopez-Rodas, G. (Gerardo)|||/items/32cdfe1e-70b1-48f3-8992-236a1d17bb83 Torres, L. (Luis)|||/items/e4740a05-e8bb-4f09-b3ca-e119dae6742b |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Dadun. Depósito Académico Digital Universidad de Navarra |
| dc.subject.none.fl_str_mv |
Cell cycle C-myc E2F Histone deacetylase Id2 Liver regeneration |
| topic |
Cell cycle C-myc E2F Histone deacetylase Id2 Liver regeneration |
| description |
The Id (inhibitor of DNA binding or inhibitor of differentiation) helix-loop-helix proteins are involved in the regulation of cell growth, differentiation and cancer. The fact that the molecular mechanisms of liver regeneration are not completely understood prompted us to study the fate of Id2 in proliferating liver. Id2 increases in liver regeneration after partial hepatectomy, following the early induction of its gene. Co-immunoprecipitation shows that Id2 forms a complex with E2F4, p130 and mSin3A in quiescent liver and all these components are present at the c-myc promoter as shown using ChIP (chromatin immunoprecipitation). Activation of c-myc during hepatocyte priming (G0-G1 transition) correlates with the dissociation of Id2 and HDAC (histone deacetylase), albeit p130 remains bound at least until 6 h. Moreover, as the G0-G1 transition progresses, Id2 and HDAC again bind the c-myc promoter concomitantly with the repression of this gene. The time course of c-myc binding to the Id2 promoter, as determined by ChIP assays is compatible with a role of the oncoprotein as a transcriptional inducer of Id2 in liver regeneration. Immunohistochemical analysis shows that Id2 also increases in proliferating hepatocytes after bile duct ligation. In this case, the pattern of Id2 presence in the c-myc promoter parallels that found in regenerating liver. Our results may suggest a control role for Id2 in hepatocyte priming, through a p130 dissociation-independent regulation of c-myc |
| publishDate |
2006 |
| dc.date.none.fl_str_mv |
2006 2006-01-01 2006 2006-01-01 2012 2012-09-27 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10171/23244 |
| url |
https://hdl.handle.net/10171/23244 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
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openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Portland Press |
| publisher.none.fl_str_mv |
Portland Press |
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reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra |
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Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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1869425486888697856 |
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15,301629 |