Three Antifungal Proteins From Penicillium expansum: Different Patterns of Production and Antifungal Activity

Antifungal proteins of fungal origin (AFPs) are small, secreted, cationic, and cysteine-rich proteins. Filamentous fungi encode a wide repertoire of AFPs belonging to different phylogenetic classes, which offer a great potential to develop new antifungals for the control of pathogenic fungi. The fun...

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Autores: Garrigues, Sandra, Gandía Gómez, Mónica, Castillo, Laia, Coca, María, Marx, Florentine, Marcos López, José Francisco, Manzanares, Paloma
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/184032
Acceso en línea:http://hdl.handle.net/10261/184032
Access Level:acceso abierto
Palabra clave:Penicillium expansum
PeAfpA
Penicillium chrysogenum
Penicillium digitatum
Botrytis cinerea
Postharvest
Crop protection
Pathogenic fungi
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spelling Three Antifungal Proteins From Penicillium expansum: Different Patterns of Production and Antifungal ActivityGarrigues, SandraGandía Gómez, MónicaCastillo, LaiaCoca, MaríaMarx, FlorentineMarcos López, José FranciscoManzanares, PalomaPenicillium expansumPeAfpAPenicillium chrysogenumPenicillium digitatumBotrytis cinereaPostharvestCrop protectionPathogenic fungiAntifungal proteins of fungal origin (AFPs) are small, secreted, cationic, and cysteine-rich proteins. Filamentous fungi encode a wide repertoire of AFPs belonging to different phylogenetic classes, which offer a great potential to develop new antifungals for the control of pathogenic fungi. The fungus Penicillium expansum is one of the few reported to encode three AFPs each belonging to a different phylogenetic class (A, B, and C). In this work, the production of the putative AFPs from P. expansum was evaluated, but only the representative of class A, PeAfpA, was identified in culture supernatants of the native fungus. The biotechnological production of PeAfpB and PeAfpC was achieved in Penicillium chrysogenum with the P. chrysogenum-based expression cassette, which had been proved to work efficiently for the production of other related AFPs in filamentous fungi. Western blot analyses confirmed that P. expansum only produces PeAfpA naturally, whereas PeAfpB and PeAfpC could not be detected. From the three AFPs from P. expansum, PeAfpA showed the highest antifungal activity against all fungi tested, including plant and human pathogens. P. expansum was also sensitive to its self-AFPs PeAfpA and PeAfpB. PeAfpB showed moderate antifungal activity against filamentous fungi, whereas no activity could be attributed to PeAfpC at the conditions tested. Importantly, none of the PeAFPs showed hemolytic activity. Finally, PeAfpA was demonstrated to efficiently protect against fungal infections caused by Botrytis cinerea in tomato leaves and Penicillium digitatum in oranges. The strong antifungal potency of PeAfpA, together with the lack of cytotoxicity, and significant in vivo protection against phytopathogenic fungi that cause postharvest decay and plant diseases, make PeAfpA a promising alternative compound for application in agriculture, but also in medicine or food preservation.This work was funded by grant BIO2015-68790-C2-1-R (to JM and PM) and BIO2015-68790-C2-2-R (to MC) from the “Ministerio de Economía y Competitividad” (Spain) (MINECO/FEDER Funds), grant PROMETEO/2018/066 (to JM and PM) from “Generalitat Valenciana” (Spain) and the Austrian Science Fund grant P25894-B20 (to FM). SG was recipient of a predoctoral scholarship (FPU13/04584) within the FPU program from “Ministerio de Educación, Cultura y Deporte” (MECD, Spain). We acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI).Peer reviewedFrontiers MediaMinisterio de Economía y Competitividad (España)European CommissionGeneralitat ValencianaAustrian Science FundMinisterio de Educación, Cultura y Deporte (España)CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/184032reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2015-68790-C2-1-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2015-68790-C2-2-Rhttps://doi.org/10.3389/fmicb.2018.02370Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1840322026-05-22T06:33:51Z
dc.title.none.fl_str_mv Three Antifungal Proteins From Penicillium expansum: Different Patterns of Production and Antifungal Activity
title Three Antifungal Proteins From Penicillium expansum: Different Patterns of Production and Antifungal Activity
spellingShingle Three Antifungal Proteins From Penicillium expansum: Different Patterns of Production and Antifungal Activity
Garrigues, Sandra
Penicillium expansum
PeAfpA
Penicillium chrysogenum
Penicillium digitatum
Botrytis cinerea
Postharvest
Crop protection
Pathogenic fungi
title_short Three Antifungal Proteins From Penicillium expansum: Different Patterns of Production and Antifungal Activity
title_full Three Antifungal Proteins From Penicillium expansum: Different Patterns of Production and Antifungal Activity
title_fullStr Three Antifungal Proteins From Penicillium expansum: Different Patterns of Production and Antifungal Activity
title_full_unstemmed Three Antifungal Proteins From Penicillium expansum: Different Patterns of Production and Antifungal Activity
title_sort Three Antifungal Proteins From Penicillium expansum: Different Patterns of Production and Antifungal Activity
dc.creator.none.fl_str_mv Garrigues, Sandra
Gandía Gómez, Mónica
Castillo, Laia
Coca, María
Marx, Florentine
Marcos López, José Francisco
Manzanares, Paloma
author Garrigues, Sandra
author_facet Garrigues, Sandra
Gandía Gómez, Mónica
Castillo, Laia
Coca, María
Marx, Florentine
Marcos López, José Francisco
Manzanares, Paloma
author_role author
author2 Gandía Gómez, Mónica
Castillo, Laia
Coca, María
Marx, Florentine
Marcos López, José Francisco
Manzanares, Paloma
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Commission
Generalitat Valenciana
Austrian Science Fund
Ministerio de Educación, Cultura y Deporte (España)
CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Penicillium expansum
PeAfpA
Penicillium chrysogenum
Penicillium digitatum
Botrytis cinerea
Postharvest
Crop protection
Pathogenic fungi
topic Penicillium expansum
PeAfpA
Penicillium chrysogenum
Penicillium digitatum
Botrytis cinerea
Postharvest
Crop protection
Pathogenic fungi
description Antifungal proteins of fungal origin (AFPs) are small, secreted, cationic, and cysteine-rich proteins. Filamentous fungi encode a wide repertoire of AFPs belonging to different phylogenetic classes, which offer a great potential to develop new antifungals for the control of pathogenic fungi. The fungus Penicillium expansum is one of the few reported to encode three AFPs each belonging to a different phylogenetic class (A, B, and C). In this work, the production of the putative AFPs from P. expansum was evaluated, but only the representative of class A, PeAfpA, was identified in culture supernatants of the native fungus. The biotechnological production of PeAfpB and PeAfpC was achieved in Penicillium chrysogenum with the P. chrysogenum-based expression cassette, which had been proved to work efficiently for the production of other related AFPs in filamentous fungi. Western blot analyses confirmed that P. expansum only produces PeAfpA naturally, whereas PeAfpB and PeAfpC could not be detected. From the three AFPs from P. expansum, PeAfpA showed the highest antifungal activity against all fungi tested, including plant and human pathogens. P. expansum was also sensitive to its self-AFPs PeAfpA and PeAfpB. PeAfpB showed moderate antifungal activity against filamentous fungi, whereas no activity could be attributed to PeAfpC at the conditions tested. Importantly, none of the PeAFPs showed hemolytic activity. Finally, PeAfpA was demonstrated to efficiently protect against fungal infections caused by Botrytis cinerea in tomato leaves and Penicillium digitatum in oranges. The strong antifungal potency of PeAfpA, together with the lack of cytotoxicity, and significant in vivo protection against phytopathogenic fungi that cause postharvest decay and plant diseases, make PeAfpA a promising alternative compound for application in agriculture, but also in medicine or food preservation.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/184032
url http://hdl.handle.net/10261/184032
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2015-68790-C2-1-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2015-68790-C2-2-R
https://doi.org/10.3389/fmicb.2018.02370
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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repository.mail.fl_str_mv
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