FoxO is required for the activation of hypertrehalosemic hormone expression in cockroaches

[Background] FoxO proteins are a subgroup of the Forkhead-box family of transcription factors, which function as the main transcriptional effectors of the insulin receptor pathway. This pathway, activated by the binding of insulin or IGFs (or insect insulin-like peptides), promotes the phosphorylati...

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Detalles Bibliográficos
Autores: Castillo, Songül Süren, Abrisqueta, Marc, Maestro, José L.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/112630
Acceso en línea:http://hdl.handle.net/10261/112630
Access Level:acceso abierto
Palabra clave:Blattella germanica
Glucagon
Hypertrehalosemic/adipokinetic hormone
Catabolism
Insulin
FoxO
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network_acronym_str ES
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spelling FoxO is required for the activation of hypertrehalosemic hormone expression in cockroachesCastillo, Songül SürenAbrisqueta, MarcMaestro, José L.Blattella germanicaGlucagonHypertrehalosemic/adipokinetic hormoneCatabolismInsulinFoxO[Background] FoxO proteins are a subgroup of the Forkhead-box family of transcription factors, which function as the main transcriptional effectors of the insulin receptor pathway. This pathway, activated by the binding of insulin or IGFs (or insect insulin-like peptides), promotes the phosphorylation and inactivation of FoxO because of its export from the nucleus to the cytoplasm. The homolog of FoxO in the cockroach Blattella germanica works in a situation of nutrient shortage by inhibiting the endocrine induction of reproduction.[Methods] Using Blattella germanica as a model, we studied the functions of FoxO using RNA interference methodologies. We analyzed the mRNA levels of hypertrehalosemic hormone (HTH) and genes related to lipolysis, glycogenolysis and gluconeogenesis and quantified triacylglycerides, glycogen and trehalose.[Results] FoxO knockdown eliminates the starvation-induced expression of HTH in the corpora cardiaca. In addition, FoxO knockdown prevents the activation of the expression of Brummer lipase, glycogen phosphorylase and phosphoenolpyruvate carboxylase in the fat body of starved females.[Conclusions] Starvation-induced activation of FoxO stimulates the transcription of different genes related to catabolic processes, including HTH and genes involved in lipolysis, glycogenolysis and gluconeogenesis.[General significance] Our results show conservation in the action of the transcription factor FoxO in the activation of catabolic processes from basal insects to vertebrates. The results also describe a new and essentially different mode of action of transcription factor FoxO, which works through the activation of neuropeptide HTH expression, which will subsequently produce its own catabolic stimulatory function. © 2013 Elsevier B.V.This work was supported by grants BFU2006-01090/BFI (Spanish Ministry of Science and Innovation (MICINN) and FEDER) and BFU2010-15906 (MICINN) to J.L.M. M.A. and S. S.-C. are recipients of a pre-doctoral fellowship (MICINN) and a post-doctoral contract (CSIC, JAE program co-funded by the European Social Fund), respectively.Peer ReviewedElsevierMinisterio de Ciencia e Innovación (España)European CommissionConsejo Superior de Investigaciones Científicas (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2015201520142015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/112630reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.bbagen.2013.08.015Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1126302026-05-22T06:33:51Z
dc.title.none.fl_str_mv FoxO is required for the activation of hypertrehalosemic hormone expression in cockroaches
title FoxO is required for the activation of hypertrehalosemic hormone expression in cockroaches
spellingShingle FoxO is required for the activation of hypertrehalosemic hormone expression in cockroaches
Castillo, Songül Süren
Blattella germanica
Glucagon
Hypertrehalosemic/adipokinetic hormone
Catabolism
Insulin
FoxO
title_short FoxO is required for the activation of hypertrehalosemic hormone expression in cockroaches
title_full FoxO is required for the activation of hypertrehalosemic hormone expression in cockroaches
title_fullStr FoxO is required for the activation of hypertrehalosemic hormone expression in cockroaches
title_full_unstemmed FoxO is required for the activation of hypertrehalosemic hormone expression in cockroaches
title_sort FoxO is required for the activation of hypertrehalosemic hormone expression in cockroaches
dc.creator.none.fl_str_mv Castillo, Songül Süren
Abrisqueta, Marc
Maestro, José L.
author Castillo, Songül Süren
author_facet Castillo, Songül Süren
Abrisqueta, Marc
Maestro, José L.
author_role author
author2 Abrisqueta, Marc
Maestro, José L.
author2_role author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
European Commission
Consejo Superior de Investigaciones Científicas (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Blattella germanica
Glucagon
Hypertrehalosemic/adipokinetic hormone
Catabolism
Insulin
FoxO
topic Blattella germanica
Glucagon
Hypertrehalosemic/adipokinetic hormone
Catabolism
Insulin
FoxO
description [Background] FoxO proteins are a subgroup of the Forkhead-box family of transcription factors, which function as the main transcriptional effectors of the insulin receptor pathway. This pathway, activated by the binding of insulin or IGFs (or insect insulin-like peptides), promotes the phosphorylation and inactivation of FoxO because of its export from the nucleus to the cytoplasm. The homolog of FoxO in the cockroach Blattella germanica works in a situation of nutrient shortage by inhibiting the endocrine induction of reproduction.
publishDate 2014
dc.date.none.fl_str_mv 2014
2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/112630
url http://hdl.handle.net/10261/112630
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.bbagen.2013.08.015
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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