Finger-prick blood sampling using volumetric absorptive microsampling (VAMS) method for monitoring the main (poly)phenolic metabolites in human blood after barley biscuit intake
A novel method based on volumetric absorptive micro-sampling (VAMS) combined with UPLC-MS/MS was developed and validated to determine the principal (poly)phenolic metabolites in human blood following the consumption of 140 g of purple whole-grain barley (WGB) biscuits. Finger-prick blood samples wer...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:10459.1/467938 |
| Acceso en línea: | https://doi.org/10.1016/j.jchromb.2025.124527 https://hdl.handle.net/10459.1/467938 |
| Access Level: | acceso abierto |
| Palabra clave: | Blood sample (poly)phenolic compound UPLC-MS/MS VAMS |
| Sumario: | A novel method based on volumetric absorptive micro-sampling (VAMS) combined with UPLC-MS/MS was developed and validated to determine the principal (poly)phenolic metabolites in human blood following the consumption of 140 g of purple whole-grain barley (WGB) biscuits. Finger-prick blood samples were collected from 11 healthy volunteers at multiple time points up to 48 h post-ingestion. To extract (poly)phenolic metabolites efficiently, various extraction parameters were optimized. Then, the method was successfully applied and five colonic (poly)phenolic metabolites from the main (poly)phenolic families from barley were detected: benzene-1,2-diol-O-sulphate, 3-(4 ′-hydroxy-3 ′-methoxy)propanoic acid and its sulphated form, 5 ′-(3 ′ ,4-dihydroxyphenyl)-γ-valerolactone-O-sulphate, and methyl luteolin-O-glucuronide. Maximum absorption occurred at 12 h for most metabolites, while luteolin-O-glucuronide showed two distinct peaks at 2 and 6 h, indicating its dual-phase absorption. Comparison with venous plasma samples collected during the 0–6 h period showed no significant differences (p > 0.05), validating the statistical reliability of VAMS as an alternative to venipuncture. Thus, VAMS emerges as a less invasive and statistically robust means for analyzing the pharmacokinetic profile of (poly)phenols, particularly those arising from colonic metabolism. |
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