Early Detection of Hyperprogressive Disease in Non-Small Cell Lung Cancer by Monitoring of Systemic T Cell Dynamics

Hyperprogressive disease (HPD) is an adverse outcome of immunotherapy consisting of an acceleration of tumor growth associated with prompt clinical deterioration. The definitions based on radiological evaluation present important technical limitations. No biomarkers have been identified yet. In this...

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Autores: Arasanz, H. (Hugo)|||/items/af58f0cb-4fe5-409c-a96a-b2a6b6831b48, Zuazo, M. (Miren)|||/items/b3adff74-ba07-454d-a65b-1c19349daa54, Bocanegra, A. (Ana)|||/items/bb0c2c14-2f4e-4fcb-a6de-a1268735f4cd, Gato-Cañas, M. (María)|||/items/ed21e1d0-319e-4b1c-a1f5-2c1be4560659, Martinez-Aguillo, M. (M.)|||/items/1a956353-bfc8-4100-896e-781680752477, Morilla, I. (Idoia)|||/items/c84da7a6-e847-47d6-9923-e5835fb0ae43, Fernández-Hinojal, G. (Gonzalo)|||/items/6a8e64fe-0006-4d63-885f-3c030209f545, Hernández, B. (Berta)|||/items/af86bb89-779a-4967-b8fe-328660ef3211, López, P. (Paúl)|||/items/8db63426-ae56-4987-8d33-e80641262ffe, Alberdi, N. (Nerea)|||/items/6769755c-4df3-406a-8f3e-c18da78e8b77, Hernández, C. (Carlos)|||/items/68e53601-57fa-4c2b-b8cd-70a92647b1f7, Chocarro, L. (Luisa)|||/items/6f7649fe-4d5c-4596-8bff-0a4c4797e891, Teijeira, L. (Lucía)|||/items/adae94b4-a30a-4c57-a728-ca13bfe3f0b6, Vera, R. (Ruth)|||/items/c5e822da-e29b-4f65-bb1c-be8bb5b999e1, Kochan, G. (Grazyna)|||/items/bf76a215-0675-467b-bb79-f2b4192fa4b2, Escors, D. (David)|||/items/82737dd6-3010-4c11-ae0f-a6efb4f177a6
Formato: artículo
Fecha de publicación:2020
País:España
Recursos:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/66188
Acesso em linha:https://hdl.handle.net/10171/66188
Access Level:acceso abierto
Palavra-chave:Hyperprogressive disease
Immunotherapy
NSCLC
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spelling Early Detection of Hyperprogressive Disease in Non-Small Cell Lung Cancer by Monitoring of Systemic T Cell DynamicsArasanz, H. (Hugo)|||/items/af58f0cb-4fe5-409c-a96a-b2a6b6831b48Zuazo, M. (Miren)|||/items/b3adff74-ba07-454d-a65b-1c19349daa54Bocanegra, A. (Ana)|||/items/bb0c2c14-2f4e-4fcb-a6de-a1268735f4cdGato-Cañas, M. (María)|||/items/ed21e1d0-319e-4b1c-a1f5-2c1be4560659Martinez-Aguillo, M. (M.)|||/items/1a956353-bfc8-4100-896e-781680752477Morilla, I. (Idoia)|||/items/c84da7a6-e847-47d6-9923-e5835fb0ae43Fernández-Hinojal, G. (Gonzalo)|||/items/6a8e64fe-0006-4d63-885f-3c030209f545Hernández, B. (Berta)|||/items/af86bb89-779a-4967-b8fe-328660ef3211López, P. (Paúl)|||/items/8db63426-ae56-4987-8d33-e80641262ffeAlberdi, N. (Nerea)|||/items/6769755c-4df3-406a-8f3e-c18da78e8b77Hernández, C. (Carlos)|||/items/68e53601-57fa-4c2b-b8cd-70a92647b1f7Chocarro, L. (Luisa)|||/items/6f7649fe-4d5c-4596-8bff-0a4c4797e891Teijeira, L. (Lucía)|||/items/adae94b4-a30a-4c57-a728-ca13bfe3f0b6Vera, R. (Ruth)|||/items/c5e822da-e29b-4f65-bb1c-be8bb5b999e1Kochan, G. (Grazyna)|||/items/bf76a215-0675-467b-bb79-f2b4192fa4b2Escors, D. (David)|||/items/82737dd6-3010-4c11-ae0f-a6efb4f177a6Hyperprogressive diseaseImmunotherapyNSCLCHyperprogressive disease (HPD) is an adverse outcome of immunotherapy consisting of an acceleration of tumor growth associated with prompt clinical deterioration. The definitions based on radiological evaluation present important technical limitations. No biomarkers have been identified yet. In this study, 70 metastatic NSCLC patients treated with anti-PD-1/PD-L1 immunotherapy after progression to platinum-based therapy were prospectively studied. Samples from peripheral blood were obtained before the first (baseline) and second cycles of treatment. Peripheral blood mononuclear cells (PBMCs) were isolated and differentiation stages of CD4 lymphocytes quantified by flow cytometry and correlated with HPD as identified with radiological criteria. A strong expansion of highly differentiated CD28− CD4 T lymphocytes (CD4 THD) between the first and second cycle of therapy was observed in HPD patients. After normalizing, the proportion of posttreatment/pretreatment CD4 THD was significantly higher in HPD when compared with the rest of patients (median 1.525 vs. 0.990; p = 0.0007), and also when stratifying by HPD, non-HPD progressors, and responders (1.525, 1.000 and 0.9700 respectively; p = 0.0025). A cut-off value of 1.3 identified HPD with 82% specificity and 70% sensitivity. An increase of CD28− CD4 T lymphocytes ≥ 1.3 (CD4 THD burst) was significantly associated with HPD (p = 0.008). The tumor growth ratio (TGR) was significantly higher in patients with expansion of CD4 THD burst compared to the rest of patients (median 2.67 vs. 0.86, p = 0.0049), and also when considering only progressors (median 2.67 vs. 1.03, p = 0.0126). A strong expansion of CD28− CD4 lymphocytes in peripheral blood within the first cycle of therapy is an early differential feature of HPD in NSCLC treated with immune-checkpoint inhibitors. The monitoring of T cell dynamics allows the early detection of this adverse outcome in clinical practice and complements radiological evaluation.Dadun. Depósito Académico Digital Universidad de Navarra20232023-05-0520202020-01-0120202020-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/66188reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/661882026-06-21T12:47:57Z
dc.title.none.fl_str_mv Early Detection of Hyperprogressive Disease in Non-Small Cell Lung Cancer by Monitoring of Systemic T Cell Dynamics
title Early Detection of Hyperprogressive Disease in Non-Small Cell Lung Cancer by Monitoring of Systemic T Cell Dynamics
spellingShingle Early Detection of Hyperprogressive Disease in Non-Small Cell Lung Cancer by Monitoring of Systemic T Cell Dynamics
Arasanz, H. (Hugo)|||/items/af58f0cb-4fe5-409c-a96a-b2a6b6831b48
Hyperprogressive disease
Immunotherapy
NSCLC
title_short Early Detection of Hyperprogressive Disease in Non-Small Cell Lung Cancer by Monitoring of Systemic T Cell Dynamics
title_full Early Detection of Hyperprogressive Disease in Non-Small Cell Lung Cancer by Monitoring of Systemic T Cell Dynamics
title_fullStr Early Detection of Hyperprogressive Disease in Non-Small Cell Lung Cancer by Monitoring of Systemic T Cell Dynamics
title_full_unstemmed Early Detection of Hyperprogressive Disease in Non-Small Cell Lung Cancer by Monitoring of Systemic T Cell Dynamics
title_sort Early Detection of Hyperprogressive Disease in Non-Small Cell Lung Cancer by Monitoring of Systemic T Cell Dynamics
dc.creator.none.fl_str_mv Arasanz, H. (Hugo)|||/items/af58f0cb-4fe5-409c-a96a-b2a6b6831b48
Zuazo, M. (Miren)|||/items/b3adff74-ba07-454d-a65b-1c19349daa54
Bocanegra, A. (Ana)|||/items/bb0c2c14-2f4e-4fcb-a6de-a1268735f4cd
Gato-Cañas, M. (María)|||/items/ed21e1d0-319e-4b1c-a1f5-2c1be4560659
Martinez-Aguillo, M. (M.)|||/items/1a956353-bfc8-4100-896e-781680752477
Morilla, I. (Idoia)|||/items/c84da7a6-e847-47d6-9923-e5835fb0ae43
Fernández-Hinojal, G. (Gonzalo)|||/items/6a8e64fe-0006-4d63-885f-3c030209f545
Hernández, B. (Berta)|||/items/af86bb89-779a-4967-b8fe-328660ef3211
López, P. (Paúl)|||/items/8db63426-ae56-4987-8d33-e80641262ffe
Alberdi, N. (Nerea)|||/items/6769755c-4df3-406a-8f3e-c18da78e8b77
Hernández, C. (Carlos)|||/items/68e53601-57fa-4c2b-b8cd-70a92647b1f7
Chocarro, L. (Luisa)|||/items/6f7649fe-4d5c-4596-8bff-0a4c4797e891
Teijeira, L. (Lucía)|||/items/adae94b4-a30a-4c57-a728-ca13bfe3f0b6
Vera, R. (Ruth)|||/items/c5e822da-e29b-4f65-bb1c-be8bb5b999e1
Kochan, G. (Grazyna)|||/items/bf76a215-0675-467b-bb79-f2b4192fa4b2
Escors, D. (David)|||/items/82737dd6-3010-4c11-ae0f-a6efb4f177a6
author Arasanz, H. (Hugo)|||/items/af58f0cb-4fe5-409c-a96a-b2a6b6831b48
author_facet Arasanz, H. (Hugo)|||/items/af58f0cb-4fe5-409c-a96a-b2a6b6831b48
Zuazo, M. (Miren)|||/items/b3adff74-ba07-454d-a65b-1c19349daa54
Bocanegra, A. (Ana)|||/items/bb0c2c14-2f4e-4fcb-a6de-a1268735f4cd
Gato-Cañas, M. (María)|||/items/ed21e1d0-319e-4b1c-a1f5-2c1be4560659
Martinez-Aguillo, M. (M.)|||/items/1a956353-bfc8-4100-896e-781680752477
Morilla, I. (Idoia)|||/items/c84da7a6-e847-47d6-9923-e5835fb0ae43
Fernández-Hinojal, G. (Gonzalo)|||/items/6a8e64fe-0006-4d63-885f-3c030209f545
Hernández, B. (Berta)|||/items/af86bb89-779a-4967-b8fe-328660ef3211
López, P. (Paúl)|||/items/8db63426-ae56-4987-8d33-e80641262ffe
Alberdi, N. (Nerea)|||/items/6769755c-4df3-406a-8f3e-c18da78e8b77
Hernández, C. (Carlos)|||/items/68e53601-57fa-4c2b-b8cd-70a92647b1f7
Chocarro, L. (Luisa)|||/items/6f7649fe-4d5c-4596-8bff-0a4c4797e891
Teijeira, L. (Lucía)|||/items/adae94b4-a30a-4c57-a728-ca13bfe3f0b6
Vera, R. (Ruth)|||/items/c5e822da-e29b-4f65-bb1c-be8bb5b999e1
Kochan, G. (Grazyna)|||/items/bf76a215-0675-467b-bb79-f2b4192fa4b2
Escors, D. (David)|||/items/82737dd6-3010-4c11-ae0f-a6efb4f177a6
author_role author
author2 Zuazo, M. (Miren)|||/items/b3adff74-ba07-454d-a65b-1c19349daa54
Bocanegra, A. (Ana)|||/items/bb0c2c14-2f4e-4fcb-a6de-a1268735f4cd
Gato-Cañas, M. (María)|||/items/ed21e1d0-319e-4b1c-a1f5-2c1be4560659
Martinez-Aguillo, M. (M.)|||/items/1a956353-bfc8-4100-896e-781680752477
Morilla, I. (Idoia)|||/items/c84da7a6-e847-47d6-9923-e5835fb0ae43
Fernández-Hinojal, G. (Gonzalo)|||/items/6a8e64fe-0006-4d63-885f-3c030209f545
Hernández, B. (Berta)|||/items/af86bb89-779a-4967-b8fe-328660ef3211
López, P. (Paúl)|||/items/8db63426-ae56-4987-8d33-e80641262ffe
Alberdi, N. (Nerea)|||/items/6769755c-4df3-406a-8f3e-c18da78e8b77
Hernández, C. (Carlos)|||/items/68e53601-57fa-4c2b-b8cd-70a92647b1f7
Chocarro, L. (Luisa)|||/items/6f7649fe-4d5c-4596-8bff-0a4c4797e891
Teijeira, L. (Lucía)|||/items/adae94b4-a30a-4c57-a728-ca13bfe3f0b6
Vera, R. (Ruth)|||/items/c5e822da-e29b-4f65-bb1c-be8bb5b999e1
Kochan, G. (Grazyna)|||/items/bf76a215-0675-467b-bb79-f2b4192fa4b2
Escors, D. (David)|||/items/82737dd6-3010-4c11-ae0f-a6efb4f177a6
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dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Hyperprogressive disease
Immunotherapy
NSCLC
topic Hyperprogressive disease
Immunotherapy
NSCLC
description Hyperprogressive disease (HPD) is an adverse outcome of immunotherapy consisting of an acceleration of tumor growth associated with prompt clinical deterioration. The definitions based on radiological evaluation present important technical limitations. No biomarkers have been identified yet. In this study, 70 metastatic NSCLC patients treated with anti-PD-1/PD-L1 immunotherapy after progression to platinum-based therapy were prospectively studied. Samples from peripheral blood were obtained before the first (baseline) and second cycles of treatment. Peripheral blood mononuclear cells (PBMCs) were isolated and differentiation stages of CD4 lymphocytes quantified by flow cytometry and correlated with HPD as identified with radiological criteria. A strong expansion of highly differentiated CD28− CD4 T lymphocytes (CD4 THD) between the first and second cycle of therapy was observed in HPD patients. After normalizing, the proportion of posttreatment/pretreatment CD4 THD was significantly higher in HPD when compared with the rest of patients (median 1.525 vs. 0.990; p = 0.0007), and also when stratifying by HPD, non-HPD progressors, and responders (1.525, 1.000 and 0.9700 respectively; p = 0.0025). A cut-off value of 1.3 identified HPD with 82% specificity and 70% sensitivity. An increase of CD28− CD4 T lymphocytes ≥ 1.3 (CD4 THD burst) was significantly associated with HPD (p = 0.008). The tumor growth ratio (TGR) was significantly higher in patients with expansion of CD4 THD burst compared to the rest of patients (median 2.67 vs. 0.86, p = 0.0049), and also when considering only progressors (median 2.67 vs. 1.03, p = 0.0126). A strong expansion of CD28− CD4 lymphocytes in peripheral blood within the first cycle of therapy is an early differential feature of HPD in NSCLC treated with immune-checkpoint inhibitors. The monitoring of T cell dynamics allows the early detection of this adverse outcome in clinical practice and complements radiological evaluation.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01
2020
2020-01-01
2023
2023-05-05
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/66188
url https://hdl.handle.net/10171/66188
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
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