TEMPO enables sequential genetic labeling and manipulation of vertebrate cell lineages

During development, regulatory factors appear in a precise order to determine cell fates over time. Consequently, to investigate complex tissue development, it is necessary to visualize and manipulate cell lineages with temporal control. Current strategies for tracing vertebrate cell lineages lack g...

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Detalles Bibliográficos
Autores: Espinosa-Medina, Isabel, Feliciano, Daniel, Belmonte Mateos, Carla, 1992-, Miyares, Rosa Linda, Garcia-Marques, Jorge, Foster, Benjamin, Lindo, Sarah, Pujades Corbi, Cristina, Koyama, Minoru, Lee, Tzumin
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/56215
Acceso en línea:http://hdl.handle.net/10230/56215
http://dx.doi.org/10.1016/j.neuron.2022.10.035
Access Level:acceso abierto
Palabra clave:CRISPR
Cell cycle
Cell fate
Cell lineage
Gliogenesis
Mouse cortex
Neurogenesis
Zebrafish hindbrain
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spelling TEMPO enables sequential genetic labeling and manipulation of vertebrate cell lineagesEspinosa-Medina, IsabelFeliciano, DanielBelmonte Mateos, Carla, 1992-Miyares, Rosa LindaGarcia-Marques, JorgeFoster, BenjaminLindo, SarahPujades Corbi, CristinaKoyama, MinoruLee, TzuminCRISPRCell cycleCell fateCell lineageGliogenesisMouse cortexNeurogenesisZebrafish hindbrainDuring development, regulatory factors appear in a precise order to determine cell fates over time. Consequently, to investigate complex tissue development, it is necessary to visualize and manipulate cell lineages with temporal control. Current strategies for tracing vertebrate cell lineages lack genetic access to sequentially produced cells. Here, we present TEMPO (Temporal Encoding and Manipulation in a Predefined Order), an imaging-readable genetic tool allowing differential labeling and manipulation of consecutive cell generations in vertebrates. TEMPO is based on CRISPR and powered by a cascade of gRNAs that drive orderly activation and inactivation of reporters and/or effectors. Using TEMPO to visualize zebrafish and mouse neurogenesis, we recapitulated birth-order-dependent neuronal fates. Temporally manipulating cell-cycle regulators in mouse cortex progenitors altered the proportion and distribution of neurons and glia, revealing the effects of temporal gene perturbation on serial cell fates. Thus, TEMPO enables sequential manipulation of molecular factors, crucial to study cell-type specification.Elsevier202320232023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/56215http://dx.doi.org/10.1016/j.neuron.2022.10.035reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésNeuron. 2023 Feb 1;111(3):345-61.e10© 2022 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/562152026-05-29T05:05:01Z
dc.title.none.fl_str_mv TEMPO enables sequential genetic labeling and manipulation of vertebrate cell lineages
title TEMPO enables sequential genetic labeling and manipulation of vertebrate cell lineages
spellingShingle TEMPO enables sequential genetic labeling and manipulation of vertebrate cell lineages
Espinosa-Medina, Isabel
CRISPR
Cell cycle
Cell fate
Cell lineage
Gliogenesis
Mouse cortex
Neurogenesis
Zebrafish hindbrain
title_short TEMPO enables sequential genetic labeling and manipulation of vertebrate cell lineages
title_full TEMPO enables sequential genetic labeling and manipulation of vertebrate cell lineages
title_fullStr TEMPO enables sequential genetic labeling and manipulation of vertebrate cell lineages
title_full_unstemmed TEMPO enables sequential genetic labeling and manipulation of vertebrate cell lineages
title_sort TEMPO enables sequential genetic labeling and manipulation of vertebrate cell lineages
dc.creator.none.fl_str_mv Espinosa-Medina, Isabel
Feliciano, Daniel
Belmonte Mateos, Carla, 1992-
Miyares, Rosa Linda
Garcia-Marques, Jorge
Foster, Benjamin
Lindo, Sarah
Pujades Corbi, Cristina
Koyama, Minoru
Lee, Tzumin
author Espinosa-Medina, Isabel
author_facet Espinosa-Medina, Isabel
Feliciano, Daniel
Belmonte Mateos, Carla, 1992-
Miyares, Rosa Linda
Garcia-Marques, Jorge
Foster, Benjamin
Lindo, Sarah
Pujades Corbi, Cristina
Koyama, Minoru
Lee, Tzumin
author_role author
author2 Feliciano, Daniel
Belmonte Mateos, Carla, 1992-
Miyares, Rosa Linda
Garcia-Marques, Jorge
Foster, Benjamin
Lindo, Sarah
Pujades Corbi, Cristina
Koyama, Minoru
Lee, Tzumin
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CRISPR
Cell cycle
Cell fate
Cell lineage
Gliogenesis
Mouse cortex
Neurogenesis
Zebrafish hindbrain
topic CRISPR
Cell cycle
Cell fate
Cell lineage
Gliogenesis
Mouse cortex
Neurogenesis
Zebrafish hindbrain
description During development, regulatory factors appear in a precise order to determine cell fates over time. Consequently, to investigate complex tissue development, it is necessary to visualize and manipulate cell lineages with temporal control. Current strategies for tracing vertebrate cell lineages lack genetic access to sequentially produced cells. Here, we present TEMPO (Temporal Encoding and Manipulation in a Predefined Order), an imaging-readable genetic tool allowing differential labeling and manipulation of consecutive cell generations in vertebrates. TEMPO is based on CRISPR and powered by a cascade of gRNAs that drive orderly activation and inactivation of reporters and/or effectors. Using TEMPO to visualize zebrafish and mouse neurogenesis, we recapitulated birth-order-dependent neuronal fates. Temporally manipulating cell-cycle regulators in mouse cortex progenitors altered the proportion and distribution of neurons and glia, revealing the effects of temporal gene perturbation on serial cell fates. Thus, TEMPO enables sequential manipulation of molecular factors, crucial to study cell-type specification.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/56215
http://dx.doi.org/10.1016/j.neuron.2022.10.035
url http://hdl.handle.net/10230/56215
http://dx.doi.org/10.1016/j.neuron.2022.10.035
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Neuron. 2023 Feb 1;111(3):345-61.e10
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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