Connecting the lab and the field: Genome analysis of phyllobacterium and rhizobium strains and field performance on two vegetable crops

12 páginas, 5 tablas, 1 figura

Detalles Bibliográficos
Autores: Flores Félix, José David, Velázquez Pérez, Encarna, Martínez Molina, Eustoquio, González-Andrés, Fernando, Squartini, Andrea, Rivas, Raúl
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/244846
Acceso en línea:http://hdl.handle.net/10261/244846
Access Level:acceso abierto
Palabra clave:Phyllobacterium
Rhizobium
Carrots
Lettuce
Bacterial endophytes
Plant growth promotion
PGPB
Genomics
Microbial biostimulants
Field assays
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spelling Connecting the lab and the field: Genome analysis of phyllobacterium and rhizobium strains and field performance on two vegetable cropsFlores Félix, José DavidVelázquez Pérez, EncarnaMartínez Molina, EustoquioGonzález-Andrés, FernandoSquartini, AndreaRivas, RaúlPhyllobacteriumRhizobiumCarrotsLettuceBacterial endophytesPlant growth promotionPGPBGenomicsMicrobial biostimulantsField assays12 páginas, 5 tablas, 1 figuraThe legume nodules are a rich source not only of rhizobia but also of endophytic bacteria exhibiting plant growth-promoting mechanisms with potential as plant biostimulants. In this work we analyzed the genomes of Phyllobacterium endophyticum PEPV15 and Rhizobium laguerreae PEPV16 strains, both isolated from Phaseolus vulgaris nodules. In silico analysis showed that the genomes of these two strains contain genes related to N-acyl-homoserine lactone (AHL) and cellulose biosynthesis, involved in quorum sensing and biofilm formation, which are essential for plant colonization. Several genes involved in plant growth promotion such as those related to phosphate solubilization, indole acetic acid production, siderophore biosynthesis and nitrogen fixation were also located in both genomes. When strains PEPV15 and PEPV16 were inoculated in lettuce and carrot in field assays, we found that both significantly increased the yield of lettuce shoots and carrot roots by more than 20% and 10%, respectively. The results of this work confirmed that the genome mining of genes involved in plant colonization and growth promotion is a good strategy for predicting the potential of bacterial strains as crops inoculants, opening new horizons for the selection of bacterial strains with which to design new, effective bacteria-based plant biostimulants.This work was supported by JCyL (Junta de Castilla y León, Spanish Regional government) grant SA183A11-2 to R.R. J.D.F.-F are granted with a FEMS grant FEMS-RG-2016-0213Peer reviewedMultidisciplinary Digital Publishing InstituteJunta de Castilla y LeónVelázquez, Encarna [0000-0002-5946-7241]Martínez Molina, Eustoquio [0000-0002-0983-9780]Rivas, Raúl [0000-0003-2202-1470]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/244846reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3390/agronomy11061124Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2448462026-05-22T06:33:51Z
dc.title.none.fl_str_mv Connecting the lab and the field: Genome analysis of phyllobacterium and rhizobium strains and field performance on two vegetable crops
title Connecting the lab and the field: Genome analysis of phyllobacterium and rhizobium strains and field performance on two vegetable crops
spellingShingle Connecting the lab and the field: Genome analysis of phyllobacterium and rhizobium strains and field performance on two vegetable crops
Flores Félix, José David
Phyllobacterium
Rhizobium
Carrots
Lettuce
Bacterial endophytes
Plant growth promotion
PGPB
Genomics
Microbial biostimulants
Field assays
title_short Connecting the lab and the field: Genome analysis of phyllobacterium and rhizobium strains and field performance on two vegetable crops
title_full Connecting the lab and the field: Genome analysis of phyllobacterium and rhizobium strains and field performance on two vegetable crops
title_fullStr Connecting the lab and the field: Genome analysis of phyllobacterium and rhizobium strains and field performance on two vegetable crops
title_full_unstemmed Connecting the lab and the field: Genome analysis of phyllobacterium and rhizobium strains and field performance on two vegetable crops
title_sort Connecting the lab and the field: Genome analysis of phyllobacterium and rhizobium strains and field performance on two vegetable crops
dc.creator.none.fl_str_mv Flores Félix, José David
Velázquez Pérez, Encarna
Martínez Molina, Eustoquio
González-Andrés, Fernando
Squartini, Andrea
Rivas, Raúl
author Flores Félix, José David
author_facet Flores Félix, José David
Velázquez Pérez, Encarna
Martínez Molina, Eustoquio
González-Andrés, Fernando
Squartini, Andrea
Rivas, Raúl
author_role author
author2 Velázquez Pérez, Encarna
Martínez Molina, Eustoquio
González-Andrés, Fernando
Squartini, Andrea
Rivas, Raúl
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Junta de Castilla y León
Velázquez, Encarna [0000-0002-5946-7241]
Martínez Molina, Eustoquio [0000-0002-0983-9780]
Rivas, Raúl [0000-0003-2202-1470]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Phyllobacterium
Rhizobium
Carrots
Lettuce
Bacterial endophytes
Plant growth promotion
PGPB
Genomics
Microbial biostimulants
Field assays
topic Phyllobacterium
Rhizobium
Carrots
Lettuce
Bacterial endophytes
Plant growth promotion
PGPB
Genomics
Microbial biostimulants
Field assays
description 12 páginas, 5 tablas, 1 figura
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/244846
url http://hdl.handle.net/10261/244846
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3390/agronomy11061124
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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