pH-sensitive phthalocyanine-loaded polymeric nanoparticles as a novel treatment strategy for breast cancer

Novel pH-sensitive polymeric photosensitizer carriers from the phthalocyanine (Pc) group were investigated as potential photodynamic therapy drugs for the treatment of breast cancer. Their high antiproliferative activity was confirmed by photocytotoxicity studies, which indicated their high efficacy...

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Authors: Malarz, Katarzyna, Borzȩcka, Wioleta, Ziola, Patryk, Domiński, Adrian, Rawicka, Patrycja, Bialik-Wąs, Katarzyna, Kurcok, Piotr, Mrozek-Wilczkiewicz, Anna, Torres Cebada, Tomás
Format: article
Publication Date:2025
Country:España
Institution:Universidad Autónoma de Madrid
Repository:Biblos-e Archivo. Repositorio Institucional de la UAM
Language:English
OAI Identifier:oai:repositorio.uam.es:10486/718097
Online Access:http://hdl.handle.net/10486/718097
https://dx.doi.org/10.1016/j.bioorg.2025.108127
Access Level:Open access
Keyword:Apoptosis
oxidative stress
photodynamic therapy
phthalocyanine-loaded polymeric nanoparticles
reactive oxygen species
Química
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spelling pH-sensitive phthalocyanine-loaded polymeric nanoparticles as a novel treatment strategy for breast cancerMalarz, KatarzynaBorzȩcka, WioletaZiola, PatrykDomiński, AdrianRawicka, PatrycjaBialik-Wąs, KatarzynaKurcok, PiotrMrozek-Wilczkiewicz, AnnaTorres Cebada, TomásApoptosisoxidative stressphotodynamic therapyphthalocyanine-loaded polymeric nanoparticlesreactive oxygen speciesQuímicaNovel pH-sensitive polymeric photosensitizer carriers from the phthalocyanine (Pc) group were investigated as potential photodynamic therapy drugs for the treatment of breast cancer. Their high antiproliferative activity was confirmed by photocytotoxicity studies, which indicated their high efficacy and specificity toward the SK-BR-3 cell line. Importantly, the Pcs encapsulated in the polymeric nanoparticle (NP) carrier exhibited a much better penetration into the acidic environment of tumor cells than their free form. The investigated Pc4-NPs and TT1-NPs exhibited a high selectivity to healthy fibroblasts as well as non-toxicity without irradiation. This paper describes the detailed mechanism of action of the evaluated compounds by measuring reactive oxygen species (ROS), including singlet oxygen; imaging cellular localization; and analyzing key signaling pathway proteins. An additional advantage of the evaluated compounds is their ability to inhibit the Akt protein expression, including its phosphorylation, which the Western blot test confirmed. This is particularly important because breast cancers often overexpress the HER-2 receptor-related signaling proteins. Moreover, an analysis of proteins such as GLUT-1, HO-1, phospho-p42/44, and BID revealed the significant involvement of ROS in disrupting cellular homeostasis, thereby leading to the induction of oxidative stress and resulting in apoptotic cell deathElsevierDepartamento de Química OrgánicaFacultad de Ciencias20252025-02-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/718097https://dx.doi.org/10.1016/j.bioorg.2025.108127reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7180972026-06-23T12:46:27Z
dc.title.none.fl_str_mv pH-sensitive phthalocyanine-loaded polymeric nanoparticles as a novel treatment strategy for breast cancer
title pH-sensitive phthalocyanine-loaded polymeric nanoparticles as a novel treatment strategy for breast cancer
spellingShingle pH-sensitive phthalocyanine-loaded polymeric nanoparticles as a novel treatment strategy for breast cancer
Malarz, Katarzyna
Apoptosis
oxidative stress
photodynamic therapy
phthalocyanine-loaded polymeric nanoparticles
reactive oxygen species
Química
title_short pH-sensitive phthalocyanine-loaded polymeric nanoparticles as a novel treatment strategy for breast cancer
title_full pH-sensitive phthalocyanine-loaded polymeric nanoparticles as a novel treatment strategy for breast cancer
title_fullStr pH-sensitive phthalocyanine-loaded polymeric nanoparticles as a novel treatment strategy for breast cancer
title_full_unstemmed pH-sensitive phthalocyanine-loaded polymeric nanoparticles as a novel treatment strategy for breast cancer
title_sort pH-sensitive phthalocyanine-loaded polymeric nanoparticles as a novel treatment strategy for breast cancer
dc.creator.none.fl_str_mv Malarz, Katarzyna
Borzȩcka, Wioleta
Ziola, Patryk
Domiński, Adrian
Rawicka, Patrycja
Bialik-Wąs, Katarzyna
Kurcok, Piotr
Mrozek-Wilczkiewicz, Anna
Torres Cebada, Tomás
author Malarz, Katarzyna
author_facet Malarz, Katarzyna
Borzȩcka, Wioleta
Ziola, Patryk
Domiński, Adrian
Rawicka, Patrycja
Bialik-Wąs, Katarzyna
Kurcok, Piotr
Mrozek-Wilczkiewicz, Anna
Torres Cebada, Tomás
author_role author
author2 Borzȩcka, Wioleta
Ziola, Patryk
Domiński, Adrian
Rawicka, Patrycja
Bialik-Wąs, Katarzyna
Kurcok, Piotr
Mrozek-Wilczkiewicz, Anna
Torres Cebada, Tomás
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Química Orgánica
Facultad de Ciencias
dc.subject.none.fl_str_mv Apoptosis
oxidative stress
photodynamic therapy
phthalocyanine-loaded polymeric nanoparticles
reactive oxygen species
Química
topic Apoptosis
oxidative stress
photodynamic therapy
phthalocyanine-loaded polymeric nanoparticles
reactive oxygen species
Química
description Novel pH-sensitive polymeric photosensitizer carriers from the phthalocyanine (Pc) group were investigated as potential photodynamic therapy drugs for the treatment of breast cancer. Their high antiproliferative activity was confirmed by photocytotoxicity studies, which indicated their high efficacy and specificity toward the SK-BR-3 cell line. Importantly, the Pcs encapsulated in the polymeric nanoparticle (NP) carrier exhibited a much better penetration into the acidic environment of tumor cells than their free form. The investigated Pc4-NPs and TT1-NPs exhibited a high selectivity to healthy fibroblasts as well as non-toxicity without irradiation. This paper describes the detailed mechanism of action of the evaluated compounds by measuring reactive oxygen species (ROS), including singlet oxygen; imaging cellular localization; and analyzing key signaling pathway proteins. An additional advantage of the evaluated compounds is their ability to inhibit the Akt protein expression, including its phosphorylation, which the Western blot test confirmed. This is particularly important because breast cancers often overexpress the HER-2 receptor-related signaling proteins. Moreover, an analysis of proteins such as GLUT-1, HO-1, phospho-p42/44, and BID revealed the significant involvement of ROS in disrupting cellular homeostasis, thereby leading to the induction of oxidative stress and resulting in apoptotic cell death
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-02-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/718097
https://dx.doi.org/10.1016/j.bioorg.2025.108127
url http://hdl.handle.net/10486/718097
https://dx.doi.org/10.1016/j.bioorg.2025.108127
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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