Transcriptomic profiling of citrus fruit peel tissues reveals fundamental effects of phenylpropanoids and ethylene on induced resistance

Penicillium spp. are the major postharvest pathogens of citrus fruit in Mediterranean climatic regions. The induction of natural resistance constitutes one of the most promising alternatives to avoid the environmental contamination and health problems caused by chemical fungicides. To understand the...

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Autores: Ballester Frutos, Ana Rosa, Lafuente, María Teresa, Forment, Javier, Gadea, José, Vos, R.C.H. de, Bovy, A.G., González-Candelas, Luis
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2011
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/127741
Acceso en línea:http://hdl.handle.net/10261/127741
Access Level:acceso abierto
Palabra clave:Secondary metabolism
Postharvest
Ethylene
Phytopathogenic fungus
Green mould
Penicillium digitatum
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spelling Transcriptomic profiling of citrus fruit peel tissues reveals fundamental effects of phenylpropanoids and ethylene on induced resistanceBallester Frutos, Ana RosaLafuente, María TeresaForment, JavierGadea, JoséVos, R.C.H. deBovy, A.G.González-Candelas, LuisSecondary metabolismPostharvestEthylenePhytopathogenic fungusGreen mouldPenicillium digitatumPenicillium spp. are the major postharvest pathogens of citrus fruit in Mediterranean climatic regions. The induction of natural resistance constitutes one of the most promising alternatives to avoid the environmental contamination and health problems caused by chemical fungicides. To understand the bases of the induction of resistance in citrus fruit against Penicillium digitatum, we have used a 12k citrus cDNA microarray to study transcriptional changes in the outer and inner parts of the peel (flavedo and albedo, respectively) of elicited fruits. The elicitor treatment led to an over-representation of biological processes associated with secondary metabolism, mainly phenylpropanoids and cellular amino acid biosynthesis and methionine metabolism, and the down-regulation of genes related to biotic and abiotic stresses. Among phenylpropanoids, we detected the over-expression of a large subset of genes important for the synthesis of flavonoids, coumarins and lignin, especially in the internal tissue. Furthermore, these genes and those of ethylene biosynthesis showed the highest induction. The involvement of both phenylpropanoid and ethylene pathways was confirmed by examining changes in gene expression and ethylene production in elicited citrus fruit. Therefore, global results indicate that secondary metabolism, mainly phenylpropanoids, and ethylene play important roles in the induction of resistance in citrus fruit.This work was supported by Research Grants AGL2002-1227 and AGL2005-04921-C02-01 from the Spanish Ministry of Science and Technology and PROMETEO/2010/010 from the Generalitat ValencianaPeer ReviewedBlackwell PublishingGeneralitat ValencianaMinisterio de Ciencia y Tecnología (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2016201620112016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/127741reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1277412026-05-22T06:33:51Z
dc.title.none.fl_str_mv Transcriptomic profiling of citrus fruit peel tissues reveals fundamental effects of phenylpropanoids and ethylene on induced resistance
title Transcriptomic profiling of citrus fruit peel tissues reveals fundamental effects of phenylpropanoids and ethylene on induced resistance
spellingShingle Transcriptomic profiling of citrus fruit peel tissues reveals fundamental effects of phenylpropanoids and ethylene on induced resistance
Ballester Frutos, Ana Rosa
Secondary metabolism
Postharvest
Ethylene
Phytopathogenic fungus
Green mould
Penicillium digitatum
title_short Transcriptomic profiling of citrus fruit peel tissues reveals fundamental effects of phenylpropanoids and ethylene on induced resistance
title_full Transcriptomic profiling of citrus fruit peel tissues reveals fundamental effects of phenylpropanoids and ethylene on induced resistance
title_fullStr Transcriptomic profiling of citrus fruit peel tissues reveals fundamental effects of phenylpropanoids and ethylene on induced resistance
title_full_unstemmed Transcriptomic profiling of citrus fruit peel tissues reveals fundamental effects of phenylpropanoids and ethylene on induced resistance
title_sort Transcriptomic profiling of citrus fruit peel tissues reveals fundamental effects of phenylpropanoids and ethylene on induced resistance
dc.creator.none.fl_str_mv Ballester Frutos, Ana Rosa
Lafuente, María Teresa
Forment, Javier
Gadea, José
Vos, R.C.H. de
Bovy, A.G.
González-Candelas, Luis
author Ballester Frutos, Ana Rosa
author_facet Ballester Frutos, Ana Rosa
Lafuente, María Teresa
Forment, Javier
Gadea, José
Vos, R.C.H. de
Bovy, A.G.
González-Candelas, Luis
author_role author
author2 Lafuente, María Teresa
Forment, Javier
Gadea, José
Vos, R.C.H. de
Bovy, A.G.
González-Candelas, Luis
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Generalitat Valenciana
Ministerio de Ciencia y Tecnología (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Secondary metabolism
Postharvest
Ethylene
Phytopathogenic fungus
Green mould
Penicillium digitatum
topic Secondary metabolism
Postharvest
Ethylene
Phytopathogenic fungus
Green mould
Penicillium digitatum
description Penicillium spp. are the major postharvest pathogens of citrus fruit in Mediterranean climatic regions. The induction of natural resistance constitutes one of the most promising alternatives to avoid the environmental contamination and health problems caused by chemical fungicides. To understand the bases of the induction of resistance in citrus fruit against Penicillium digitatum, we have used a 12k citrus cDNA microarray to study transcriptional changes in the outer and inner parts of the peel (flavedo and albedo, respectively) of elicited fruits. The elicitor treatment led to an over-representation of biological processes associated with secondary metabolism, mainly phenylpropanoids and cellular amino acid biosynthesis and methionine metabolism, and the down-regulation of genes related to biotic and abiotic stresses. Among phenylpropanoids, we detected the over-expression of a large subset of genes important for the synthesis of flavonoids, coumarins and lignin, especially in the internal tissue. Furthermore, these genes and those of ethylene biosynthesis showed the highest induction. The involvement of both phenylpropanoid and ethylene pathways was confirmed by examining changes in gene expression and ethylene production in elicited citrus fruit. Therefore, global results indicate that secondary metabolism, mainly phenylpropanoids, and ethylene play important roles in the induction of resistance in citrus fruit.
publishDate 2011
dc.date.none.fl_str_mv 2011
2016
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/127741
url http://hdl.handle.net/10261/127741
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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