Characterizing the involvement of FaMADS9 in the regulation of strawberry fruit receptacle development

© 2019 The Authors.

Detalhes bibliográficos
Autores: Vallarino, José G., Merchante, Catharina, Sánchez-Sevilla, José F, de Luis Balaguer, María Angels, Pott, Delphine M., Ariza Fernández, María Teresa, Casañal, Ana, Posé, David, Vioque, Amailia, Amaya, Iraida, Willmitzer, Lothar, Solano, Roberto, Sozzani, Rosangela, Fernie, Alisdair R., Botella, Miguel Angel, Giovannoni, James J., Valpuesta, Victoriano, Osorio, Sonia
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/228808
Acesso em linha:http://hdl.handle.net/10261/228808
Access Level:acceso abierto
Palavra-chave:Strawberry
Fruit ripening
Quality
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spelling Characterizing the involvement of FaMADS9 in the regulation of strawberry fruit receptacle developmentVallarino, José G.Merchante, CatharinaSánchez-Sevilla, José Fde Luis Balaguer, María AngelsPott, Delphine M.Ariza Fernández, María TeresaCasañal, AnaPosé, DavidVioque, AmailiaAmaya, IraidaWillmitzer, LotharSolano, RobertoSozzani, RosangelaFernie, Alisdair R.Botella, Miguel AngelGiovannoni, James J.Valpuesta, VictorianoOsorio, SoniaStrawberryFruit ripeningQuality© 2019 The Authors.FaMADS9 is the strawberry (Fragaria x ananassa) gene that exhibits the highest homology to the tomato (Solanum lycopersicum) RIN gene. Transgenic lines were obtained in which FaMADS9 was silenced. The fruits of these lines did not show differences in basic parameters, such as fruit firmness or colour, but exhibited lower Brix values in three of the four independent lines. The gene ontology MapMan category that was most enriched among the differentially expressed genes in the receptacles at the white stage corresponded to the regulation of transcription, including a high percentage of transcription factors and regulatory proteins associated with auxin action. In contrast, the most enriched categories at the red stage were transport, lipid metabolism and cell wall. Metabolomic analysis of the receptacles of the transformed fruits identified significant changes in the content of maltose, galactonic acid‐1,4‐lactone, proanthocyanidins and flavonols at the green/white stage, while isomaltose, anthocyanins and cuticular wax metabolism were the most affected at the red stage. Among the regulatory genes that were differentially expressed in the transgenic receptacles were several genes previously linked to flavonoid metabolism, such as MYB10, DIV, ZFN1, ZFN2, GT2, and GT5, or associated with the action of hormones, such as abscisic acid, SHP, ASR, GTE7 and SnRK2.7. The inference of a gene regulatory network, based on a dynamic Bayesian approach, among the genes differentially expressed in the transgenic receptacles at the white and red stages, identified the genes KAN1, DIV, ZFN2 and GTE7 as putative targets of FaMADS9. A MADS9‐specific CArG box was identified in the promoters of these genes.This work was supported by grants BIO2013-44199-R, AGR12- 40066-CO2-02, and RTI2018-099797-B-100 (MINECO, Spain), and the European Union’s H2020 Programme (GoodBerry; grant number 679303) and ERC-2014-StG638134. DP and SO acknowledge the support by Spanish Ministry of Science and Innovation (Ramon and Cajal contracts, RYC2011-09170 and RYC2013-12699).John Wiley & SonsMinisterio de Economía y Competitividad (España)Agencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120202021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/228808reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2013-44199-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099797-B-100RTI2018-099797-B-100/AEI/10.13039/501100011033info:eu-repo/grantAgreement/EC/H2020/679303info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RYC2013-12699http://dx.doi.org/10.1111/pbi.13257Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2288082026-05-22T06:33:51Z
dc.title.none.fl_str_mv Characterizing the involvement of FaMADS9 in the regulation of strawberry fruit receptacle development
title Characterizing the involvement of FaMADS9 in the regulation of strawberry fruit receptacle development
spellingShingle Characterizing the involvement of FaMADS9 in the regulation of strawberry fruit receptacle development
Vallarino, José G.
Strawberry
Fruit ripening
Quality
title_short Characterizing the involvement of FaMADS9 in the regulation of strawberry fruit receptacle development
title_full Characterizing the involvement of FaMADS9 in the regulation of strawberry fruit receptacle development
title_fullStr Characterizing the involvement of FaMADS9 in the regulation of strawberry fruit receptacle development
title_full_unstemmed Characterizing the involvement of FaMADS9 in the regulation of strawberry fruit receptacle development
title_sort Characterizing the involvement of FaMADS9 in the regulation of strawberry fruit receptacle development
dc.creator.none.fl_str_mv Vallarino, José G.
Merchante, Catharina
Sánchez-Sevilla, José F
de Luis Balaguer, María Angels
Pott, Delphine M.
Ariza Fernández, María Teresa
Casañal, Ana
Posé, David
Vioque, Amailia
Amaya, Iraida
Willmitzer, Lothar
Solano, Roberto
Sozzani, Rosangela
Fernie, Alisdair R.
Botella, Miguel Angel
Giovannoni, James J.
Valpuesta, Victoriano
Osorio, Sonia
author Vallarino, José G.
author_facet Vallarino, José G.
Merchante, Catharina
Sánchez-Sevilla, José F
de Luis Balaguer, María Angels
Pott, Delphine M.
Ariza Fernández, María Teresa
Casañal, Ana
Posé, David
Vioque, Amailia
Amaya, Iraida
Willmitzer, Lothar
Solano, Roberto
Sozzani, Rosangela
Fernie, Alisdair R.
Botella, Miguel Angel
Giovannoni, James J.
Valpuesta, Victoriano
Osorio, Sonia
author_role author
author2 Merchante, Catharina
Sánchez-Sevilla, José F
de Luis Balaguer, María Angels
Pott, Delphine M.
Ariza Fernández, María Teresa
Casañal, Ana
Posé, David
Vioque, Amailia
Amaya, Iraida
Willmitzer, Lothar
Solano, Roberto
Sozzani, Rosangela
Fernie, Alisdair R.
Botella, Miguel Angel
Giovannoni, James J.
Valpuesta, Victoriano
Osorio, Sonia
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Strawberry
Fruit ripening
Quality
topic Strawberry
Fruit ripening
Quality
description © 2019 The Authors.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/228808
url http://hdl.handle.net/10261/228808
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2013-44199-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099797-B-100
RTI2018-099797-B-100/AEI/10.13039/501100011033
info:eu-repo/grantAgreement/EC/H2020/679303
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RYC2013-12699
http://dx.doi.org/10.1111/pbi.13257
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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