N-Methyl-N-((1-methyl-5-(3-(piperidin-1-yl)propoxy)-1H-benzo[d]imidazol-2-yl)methyl)prop-2-yn-1-amine (MBA-159), a new multitarget small molecule for the therapy of Alzheimer's disease

As part of a project aimed at the pharmacological optimization of Contilisant, herein we describe molecular modelling studies that led to the identification of MBA-159 as a new polyfunctionalized, multitarget-directed ligand and a promising drug candidate for the treatment of Alzheimer's diseas...

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Autores: Bautista-Aguilera, Óscar M., Manik, Aleksandra, Diez-Iriepa, Daniel, Szałaj, Natalia, Zaręba, Paula, Więckowska, Anna, Żmudzki, Paweł, Honkisz-Orzechowska, Ewelina, Knez, Damijan, Gobec, Stanislav, Sałat, Kinga, Martínez-Alonso, Borja, Guarnizo-Herrero, Víctor, Durán, Guillermo Torrado, Torrado-Salmerón, Carlos, Bellver-Sanchis, Aina, Nsiona-Defise, Inaya, Ribalta-Vilella, Marta, Pallàs, Mercè, López-Muñoz, Francisco, Griñán-Ferré, Christian, Marco-Contelles, José, Iriepa, Isabel
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2025
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/402078
Acesso em linha:http://hdl.handle.net/10261/402078
https://api.elsevier.com/content/abstract/scopus_id/105017041855
Access Level:Acceso aberto
Palavra-chave:ADME
Alzheimer’s disease
Biological evaluation
Molecular modelling
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dc.title.none.fl_str_mv N-Methyl-N-((1-methyl-5-(3-(piperidin-1-yl)propoxy)-1H-benzo[d]imidazol-2-yl)methyl)prop-2-yn-1-amine (MBA-159), a new multitarget small molecule for the therapy of Alzheimer's disease
title N-Methyl-N-((1-methyl-5-(3-(piperidin-1-yl)propoxy)-1H-benzo[d]imidazol-2-yl)methyl)prop-2-yn-1-amine (MBA-159), a new multitarget small molecule for the therapy of Alzheimer's disease
spellingShingle N-Methyl-N-((1-methyl-5-(3-(piperidin-1-yl)propoxy)-1H-benzo[d]imidazol-2-yl)methyl)prop-2-yn-1-amine (MBA-159), a new multitarget small molecule for the therapy of Alzheimer's disease
Bautista-Aguilera, Óscar M.
ADME
Alzheimer’s disease
Biological evaluation
Molecular modelling
title_short N-Methyl-N-((1-methyl-5-(3-(piperidin-1-yl)propoxy)-1H-benzo[d]imidazol-2-yl)methyl)prop-2-yn-1-amine (MBA-159), a new multitarget small molecule for the therapy of Alzheimer's disease
title_full N-Methyl-N-((1-methyl-5-(3-(piperidin-1-yl)propoxy)-1H-benzo[d]imidazol-2-yl)methyl)prop-2-yn-1-amine (MBA-159), a new multitarget small molecule for the therapy of Alzheimer's disease
title_fullStr N-Methyl-N-((1-methyl-5-(3-(piperidin-1-yl)propoxy)-1H-benzo[d]imidazol-2-yl)methyl)prop-2-yn-1-amine (MBA-159), a new multitarget small molecule for the therapy of Alzheimer's disease
title_full_unstemmed N-Methyl-N-((1-methyl-5-(3-(piperidin-1-yl)propoxy)-1H-benzo[d]imidazol-2-yl)methyl)prop-2-yn-1-amine (MBA-159), a new multitarget small molecule for the therapy of Alzheimer's disease
title_sort N-Methyl-N-((1-methyl-5-(3-(piperidin-1-yl)propoxy)-1H-benzo[d]imidazol-2-yl)methyl)prop-2-yn-1-amine (MBA-159), a new multitarget small molecule for the therapy of Alzheimer's disease
dc.creator.none.fl_str_mv Bautista-Aguilera, Óscar M.
Manik, Aleksandra
Diez-Iriepa, Daniel
Szałaj, Natalia
Zaręba, Paula
Więckowska, Anna
Żmudzki, Paweł
Honkisz-Orzechowska, Ewelina
Knez, Damijan
Gobec, Stanislav
Sałat, Kinga
Martínez-Alonso, Borja
Guarnizo-Herrero, Víctor
Durán, Guillermo Torrado
Torrado-Salmerón, Carlos
Bellver-Sanchis, Aina
Nsiona-Defise, Inaya
Ribalta-Vilella, Marta
Pallàs, Mercè
López-Muñoz, Francisco
Griñán-Ferré, Christian
Marco-Contelles, José
Iriepa, Isabel
author Bautista-Aguilera, Óscar M.
author_facet Bautista-Aguilera, Óscar M.
Manik, Aleksandra
Diez-Iriepa, Daniel
Szałaj, Natalia
Zaręba, Paula
Więckowska, Anna
Żmudzki, Paweł
Honkisz-Orzechowska, Ewelina
Knez, Damijan
Gobec, Stanislav
Sałat, Kinga
Martínez-Alonso, Borja
Guarnizo-Herrero, Víctor
Durán, Guillermo Torrado
Torrado-Salmerón, Carlos
Bellver-Sanchis, Aina
Nsiona-Defise, Inaya
Ribalta-Vilella, Marta
Pallàs, Mercè
López-Muñoz, Francisco
Griñán-Ferré, Christian
Marco-Contelles, José
Iriepa, Isabel
author_role author
author2 Manik, Aleksandra
Diez-Iriepa, Daniel
Szałaj, Natalia
Zaręba, Paula
Więckowska, Anna
Żmudzki, Paweł
Honkisz-Orzechowska, Ewelina
Knez, Damijan
Gobec, Stanislav
Sałat, Kinga
Martínez-Alonso, Borja
Guarnizo-Herrero, Víctor
Durán, Guillermo Torrado
Torrado-Salmerón, Carlos
Bellver-Sanchis, Aina
Nsiona-Defise, Inaya
Ribalta-Vilella, Marta
Pallàs, Mercè
López-Muñoz, Francisco
Griñán-Ferré, Christian
Marco-Contelles, José
Iriepa, Isabel
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Ministerio de Economía, Industria y Competitividad (España)
Generalitat de Catalunya
European Commission
National Science Centre (Poland)
Ministry of Education, Science and Sport (Slovenia)
Bautista-Aguilera, Óscar M. [0000-0002-1327-1681]
Diez-Iriepa, Daniel [0000-0002-8575-1246]
Żmudzki, Paweł [0000-0003-3594-124]
Honkisz-Orzechowska, Ewelina [0000-0002-2046-4879]
Knez, Damijan [0000-0001-9917-1384]
Gobec, Stanislav [0000-0002-9678-3083]
Martínez-Alonso, Borja [0009-0002-9124-5507]
Guarnizo-Herrero, Víctor [0000-0001-9323-3388]
Nsiona-Defise, Inaya [0009-0001-0596-315]
Pallàs, Mercè [0000-0003-3095-4254]
Marco-Contelles, José [0000-0003-0690-0328]
Iriepa, Isabel [0000-0003-3475-9624]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv ADME
Alzheimer’s disease
Biological evaluation
Molecular modelling
topic ADME
Alzheimer’s disease
Biological evaluation
Molecular modelling
description As part of a project aimed at the pharmacological optimization of Contilisant, herein we describe molecular modelling studies that led to the identification of MBA-159 as a new polyfunctionalized, multitarget-directed ligand and a promising drug candidate for the treatment of Alzheimer's disease. We synthesized MBA-159 and conducted comprehensive in vitro and in vivo evaluations. In in vivo studies MBA-159 demonstrated favourable pharmacokinetics, anti-amnesic properties and significantly improved non-spatial memory (contextual and recognition memory) in a mouse model of scopolamine-induced amnesia. Additionally, MBA-159 showed a tendency to increase synaptic plasticity biomarkers and reduce neuroinflammatory trends (assessed by qPCR), as well as cognitive enhancement in a senescence-accelerated prone mouse 8 model.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/402078
https://api.elsevier.com/content/abstract/scopus_id/105017041855
url http://hdl.handle.net/10261/402078
https://api.elsevier.com/content/abstract/scopus_id/105017041855
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.biopha.2025.118603

dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869425418347479040
spelling N-Methyl-N-((1-methyl-5-(3-(piperidin-1-yl)propoxy)-1H-benzo[d]imidazol-2-yl)methyl)prop-2-yn-1-amine (MBA-159), a new multitarget small molecule for the therapy of Alzheimer's diseaseBautista-Aguilera, Óscar M.Manik, AleksandraDiez-Iriepa, DanielSzałaj, NataliaZaręba, PaulaWięckowska, AnnaŻmudzki, PawełHonkisz-Orzechowska, EwelinaKnez, DamijanGobec, StanislavSałat, KingaMartínez-Alonso, BorjaGuarnizo-Herrero, VíctorDurán, Guillermo TorradoTorrado-Salmerón, CarlosBellver-Sanchis, AinaNsiona-Defise, InayaRibalta-Vilella, MartaPallàs, MercèLópez-Muñoz, FranciscoGriñán-Ferré, ChristianMarco-Contelles, JoséIriepa, IsabelADMEAlzheimer’s diseaseBiological evaluationMolecular modellingAs part of a project aimed at the pharmacological optimization of Contilisant, herein we describe molecular modelling studies that led to the identification of MBA-159 as a new polyfunctionalized, multitarget-directed ligand and a promising drug candidate for the treatment of Alzheimer's disease. We synthesized MBA-159 and conducted comprehensive in vitro and in vivo evaluations. In in vivo studies MBA-159 demonstrated favourable pharmacokinetics, anti-amnesic properties and significantly improved non-spatial memory (contextual and recognition memory) in a mouse model of scopolamine-induced amnesia. Additionally, MBA-159 showed a tendency to increase synaptic plasticity biomarkers and reduce neuroinflammatory trends (assessed by qPCR), as well as cognitive enhancement in a senescence-accelerated prone mouse 8 model.II thanks AEI (Government of Spain; Grant PID2019–105813RB- C22). JMC thanks AEI (Government of Spain; Grant PID2019–105813RB-C21) and UCJC [Grant: “In vivo analysis of new analogues of Contilisant (MITOPI) (2022)”] for support. This study was also supported by the Ministerio de Economía, Industria y Com- petitividad (Agencia Estatal de Investigación, AEI; to CGF, and MP) and Fondo Europeo de Desarrollo Regional (MINECO-FEDER) (PID2022–139016OA-I00, PDC2022–133441-I00, to CGF, and MP), Generalitat de Catalunya (2021 SGR 00357; to CGF and MP). This study was co-financed by Secretaria d’Universitats i Recerca del Departament d’Empresa i Coneixement de la Generalitat de Catalunya 2022 (Producte 0092; Llavor 005 and 007; to CGF). The Government of Catalonia (2021 SGR 00357 to C.G.-F and M.P.). II and OMBA thank Laura Contreras andCristina García for their collaboration. II and OMBA thank subsidies for the implementation of the research program within the framework of the recovery, transformation and resilience plan – financed by the Eu- ropean Union – Next Generation EU. CAM. K.S. and E.H.-O. thank JUMC for support (Grant N42/DBS/000290 and N42/DBS/000332). ADMET studies were financed by the National Science Centre Poland (Grant No. 2019/34/E/NZ7/00090). The ADMET research/study were carried out with the use of research infrastructure co-financed by the Smart Growth Operational Programme POIR 4.2 project no. POIR.04.02.00–00-D023/ 20. The financial support from the Slovenian Research and Innovation Agency (Research Core Funding grant P1–0208) is gratefully acknowl- edged. This research was supported by the Ministry of Education, Sci- ence, and Sport (MIZˇS) and the European Regional Development Fund OP20.05187 RI-SI-EATRIS. BMA, VGH, CTS and GTD, thanks UAH CEA and CEMAETA CAI for the in vivo pharmacokinetics studies which were carried out with the use of their research infrastructure.Peer reviewedElsevierMinisterio de Ciencia e Innovación (España)Ministerio de Economía, Industria y Competitividad (España)Generalitat de CatalunyaEuropean CommissionNational Science Centre (Poland)Ministry of Education, Science and Sport (Slovenia)Bautista-Aguilera, Óscar M. [0000-0002-1327-1681]Diez-Iriepa, Daniel [0000-0002-8575-1246]Żmudzki, Paweł [0000-0003-3594-124]Honkisz-Orzechowska, Ewelina [0000-0002-2046-4879]Knez, Damijan [0000-0001-9917-1384]Gobec, Stanislav [0000-0002-9678-3083]Martínez-Alonso, Borja [0009-0002-9124-5507]Guarnizo-Herrero, Víctor [0000-0001-9323-3388]Nsiona-Defise, Inaya [0009-0001-0596-315]Pallàs, Mercè [0000-0003-3095-4254]Marco-Contelles, José [0000-0003-0690-0328]Iriepa, Isabel [0000-0003-3475-9624]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/402078https://api.elsevier.com/content/abstract/scopus_id/105017041855reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/j.biopha.2025.118603Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4020782026-05-22T06:33:51Z
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