High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)

Tropical gar (Atractosteus tropicus) is freshwater and estuarine fish, inhabiting the Earth since the Mesozoic era and undergoing limited physiological variation ever since. Besides its recognized cultural and scientific relevance, the species has seen remarkable growth in its economic impact due to...

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Autores: Mendez-Perez, R, Garcia-Lopez, R, Bautista-Lopez, JS, Vazquez-Castellanos, J, Alvarez-Gonzalez, C, Pena-Marin, E, Baltierra-Trejo, E, Adams-Schroeder, R, Dominguez-Rodriguez, V, Melgar-Valdes, C, Martinez-Garcia, R, Moya, A, Gomez-Cruz, R
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
Repositorio:r-FISABIO. Repositorio Institucional de Producción Científica
OAI Identifier:oai:fisabio.fundanetsuite.com:p9470
Acceso en línea:https://fisabio.portalinvestigacion.com/publicaciones/9470
Access Level:acceso abierto
Palabra clave:Atractosteus tropicus
gut microbiome
metagenomics
16S rRNA profiling
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spelling High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)Mendez-Perez, RGarcia-Lopez, RBautista-Lopez, JSVazquez-Castellanos, JAlvarez-Gonzalez, CPena-Marin, EBaltierra-Trejo, EAdams-Schroeder, RDominguez-Rodriguez, VMelgar-Valdes, CMartinez-Garcia, RMoya, AGomez-Cruz, RAtractosteus tropicusgut microbiomemetagenomics16S rRNA profilingTropical gar (Atractosteus tropicus) is freshwater and estuarine fish, inhabiting the Earth since the Mesozoic era and undergoing limited physiological variation ever since. Besides its recognized cultural and scientific relevance, the species has seen remarkable growth in its economic impact due to pisciculture. In this study, we present the first report of the whole taxonomic composition of microbial communities in gut contents in juveniles and adults of A. tropicus, by sex and origin (wild and cultivated). For this study, 508 genera were identified, with the most and least abundant being Cetobacterium and Paludibacter, respectively. Fusobacteria, Proteobacteria, Firmicutes, and Bacteroidetes phyla are the core gut microbiome of A. tropicus juvenile and adult by sex and origin. Deinococcus-Thermus phylum sequence was only identified in wild-type males. In the phylogenetic trees reconstruction Lactococcus lactis strains CAU929 and CAU6600, Cp6 and CAU9951, Cetobacterium strain H69, Aeromonas hydrophila strain P5 and WR-5-3-2, Aeromonas sobria strain CP DC28 and Aeromonas hydrophila were identified, some of them with probiotic potential within the three dominant phyla in core gut microbiome in A. tropicus adults, especially in wild-type organisms. Myroides genus was recognized in microbiota gut of the cultivated juvenile A. tropicus. Nevertheless, Alpha diversity indicated that the highest gut microbiota abundance and richness is found in cultivated juvenile and wild-type adult A. tropicus female, rather than adult wild-type males and the least gut microbiota abundance and richness is found in a cultivated adult of A. tropicus for both sexes.PONTIFICIA UNIV CATOLICA VALPARAISO2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fisabio.portalinvestigacion.com/publicaciones/9470Latin American Journal of Aquatic ResearchISSN: 0718560Xreponame:r-FISABIO. Repositorio Institucional de Producción Científicainstname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)Españolinfo:eu-repo/semantics/openAccessoai:fisabio.fundanetsuite.com:p94702026-06-11T12:45:17Z
dc.title.none.fl_str_mv High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)
title High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)
spellingShingle High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)
Mendez-Perez, R
Atractosteus tropicus
gut microbiome
metagenomics
16S rRNA profiling
title_short High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)
title_full High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)
title_fullStr High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)
title_full_unstemmed High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)
title_sort High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)
dc.creator.none.fl_str_mv Mendez-Perez, R
Garcia-Lopez, R
Bautista-Lopez, JS
Vazquez-Castellanos, J
Alvarez-Gonzalez, C
Pena-Marin, E
Baltierra-Trejo, E
Adams-Schroeder, R
Dominguez-Rodriguez, V
Melgar-Valdes, C
Martinez-Garcia, R
Moya, A
Gomez-Cruz, R
author Mendez-Perez, R
author_facet Mendez-Perez, R
Garcia-Lopez, R
Bautista-Lopez, JS
Vazquez-Castellanos, J
Alvarez-Gonzalez, C
Pena-Marin, E
Baltierra-Trejo, E
Adams-Schroeder, R
Dominguez-Rodriguez, V
Melgar-Valdes, C
Martinez-Garcia, R
Moya, A
Gomez-Cruz, R
author_role author
author2 Garcia-Lopez, R
Bautista-Lopez, JS
Vazquez-Castellanos, J
Alvarez-Gonzalez, C
Pena-Marin, E
Baltierra-Trejo, E
Adams-Schroeder, R
Dominguez-Rodriguez, V
Melgar-Valdes, C
Martinez-Garcia, R
Moya, A
Gomez-Cruz, R
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Atractosteus tropicus
gut microbiome
metagenomics
16S rRNA profiling
topic Atractosteus tropicus
gut microbiome
metagenomics
16S rRNA profiling
description Tropical gar (Atractosteus tropicus) is freshwater and estuarine fish, inhabiting the Earth since the Mesozoic era and undergoing limited physiological variation ever since. Besides its recognized cultural and scientific relevance, the species has seen remarkable growth in its economic impact due to pisciculture. In this study, we present the first report of the whole taxonomic composition of microbial communities in gut contents in juveniles and adults of A. tropicus, by sex and origin (wild and cultivated). For this study, 508 genera were identified, with the most and least abundant being Cetobacterium and Paludibacter, respectively. Fusobacteria, Proteobacteria, Firmicutes, and Bacteroidetes phyla are the core gut microbiome of A. tropicus juvenile and adult by sex and origin. Deinococcus-Thermus phylum sequence was only identified in wild-type males. In the phylogenetic trees reconstruction Lactococcus lactis strains CAU929 and CAU6600, Cp6 and CAU9951, Cetobacterium strain H69, Aeromonas hydrophila strain P5 and WR-5-3-2, Aeromonas sobria strain CP DC28 and Aeromonas hydrophila were identified, some of them with probiotic potential within the three dominant phyla in core gut microbiome in A. tropicus adults, especially in wild-type organisms. Myroides genus was recognized in microbiota gut of the cultivated juvenile A. tropicus. Nevertheless, Alpha diversity indicated that the highest gut microbiota abundance and richness is found in cultivated juvenile and wild-type adult A. tropicus female, rather than adult wild-type males and the least gut microbiota abundance and richness is found in a cultivated adult of A. tropicus for both sexes.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fisabio.portalinvestigacion.com/publicaciones/9470
url https://fisabio.portalinvestigacion.com/publicaciones/9470
dc.language.none.fl_str_mv Español
language_invalid_str_mv Español
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv PONTIFICIA UNIV CATOLICA VALPARAISO
publisher.none.fl_str_mv PONTIFICIA UNIV CATOLICA VALPARAISO
dc.source.none.fl_str_mv Latin American Journal of Aquatic Research
ISSN: 0718560X
reponame:r-FISABIO. Repositorio Institucional de Producción Científica
instname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
instname_str Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
reponame_str r-FISABIO. Repositorio Institucional de Producción Científica
collection r-FISABIO. Repositorio Institucional de Producción Científica
repository.name.fl_str_mv
repository.mail.fl_str_mv
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