Engineering alginate-based injectable hydrogels combined with bioactive polymers for targeted plasma-derived oxidative stress delivery in osteosarcoma therapy

Reactive Oxygen and Nitrogen Species (RONS) in biological systems display hormetic effects, capable of either promoting cell regenerative effects or inducing cell death. Recently, hydrogels have emerged as a promising delivery platform for RONS generated from Cold Atmospheric Plasmas (CAP), known as...

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Autores: Espona Noguera, Albert|||0000-0002-3681-030X, Tampieri, Francesco|||0000-0003-1474-867X, Canal Barnils, Cristina|||0000-0002-3039-7462
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/408685
Acceso en línea:https://hdl.handle.net/2117/408685
https://dx.doi.org/10.1016/j.ijbiomac.2023.128841
Access Level:acceso abierto
Palabra clave:Colloids in medicine
Tissue engineering
Col·loides en medicina
Enginyeria de teixits
Àrees temàtiques de la UPC::Enginyeria biomèdica
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spelling Engineering alginate-based injectable hydrogels combined with bioactive polymers for targeted plasma-derived oxidative stress delivery in osteosarcoma therapyEspona Noguera, Albert|||0000-0002-3681-030XTampieri, Francesco|||0000-0003-1474-867XCanal Barnils, Cristina|||0000-0002-3039-7462Colloids in medicineTissue engineeringCol·loides en medicinaEnginyeria de teixitsÀrees temàtiques de la UPC::Enginyeria biomèdicaReactive Oxygen and Nitrogen Species (RONS) in biological systems display hormetic effects, capable of either promoting cell regenerative effects or inducing cell death. Recently, hydrogels have emerged as a promising delivery platform for RONS generated from Cold Atmospheric Plasmas (CAP), known as Plasma-Treated Hydrogels (PTH). PTH have been proposed as an alternative therapy to conventional cancer treatments, offering reduced side effects through the controlled and localized delivery of plasma-derived RONS. In this work, we have developed alginate-based PTH with dual therapeutic action provided by plasma-derived RONS acting as selective anticancer agents for osteosarcoma treatment, and biomolecules (hyaluronic acid and gelatin) to promote stem cell-mediated bone regeneration. For this purpose, we designed a novel manufacturing process to maximize the load of plasma-derived RONS within the PTH. Then, we assessed the PTH bioactivity on osteosarcoma MG-63 cells, and human mesenchymal stem cells (hMSCs). The results showed that the PTH composed of 0.25 % alginate +1 % hyaluronic acid is the most promising formulation in osteosarcoma treatment, showing a dual-action bioactivity as a selective cytotoxic anticancer agent, and as promoter of the proliferation and osteogenic differentiation of hMSCs. These findings provide strong evidence of the significant potential of PTH in the oncological field.Peer ReviewedObjectius de Desenvolupament Sostenible::3 - Salut i BenestarElsevier20242024-02-0120242024-05-28journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/408685https://dx.doi.org/10.1016/j.ijbiomac.2023.128841reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/4086852026-05-27T15:37:01Z
dc.title.none.fl_str_mv Engineering alginate-based injectable hydrogels combined with bioactive polymers for targeted plasma-derived oxidative stress delivery in osteosarcoma therapy
title Engineering alginate-based injectable hydrogels combined with bioactive polymers for targeted plasma-derived oxidative stress delivery in osteosarcoma therapy
spellingShingle Engineering alginate-based injectable hydrogels combined with bioactive polymers for targeted plasma-derived oxidative stress delivery in osteosarcoma therapy
Espona Noguera, Albert|||0000-0002-3681-030X
Colloids in medicine
Tissue engineering
Col·loides en medicina
Enginyeria de teixits
Àrees temàtiques de la UPC::Enginyeria biomèdica
title_short Engineering alginate-based injectable hydrogels combined with bioactive polymers for targeted plasma-derived oxidative stress delivery in osteosarcoma therapy
title_full Engineering alginate-based injectable hydrogels combined with bioactive polymers for targeted plasma-derived oxidative stress delivery in osteosarcoma therapy
title_fullStr Engineering alginate-based injectable hydrogels combined with bioactive polymers for targeted plasma-derived oxidative stress delivery in osteosarcoma therapy
title_full_unstemmed Engineering alginate-based injectable hydrogels combined with bioactive polymers for targeted plasma-derived oxidative stress delivery in osteosarcoma therapy
title_sort Engineering alginate-based injectable hydrogels combined with bioactive polymers for targeted plasma-derived oxidative stress delivery in osteosarcoma therapy
dc.creator.none.fl_str_mv Espona Noguera, Albert|||0000-0002-3681-030X
Tampieri, Francesco|||0000-0003-1474-867X
Canal Barnils, Cristina|||0000-0002-3039-7462
author Espona Noguera, Albert|||0000-0002-3681-030X
author_facet Espona Noguera, Albert|||0000-0002-3681-030X
Tampieri, Francesco|||0000-0003-1474-867X
Canal Barnils, Cristina|||0000-0002-3039-7462
author_role author
author2 Tampieri, Francesco|||0000-0003-1474-867X
Canal Barnils, Cristina|||0000-0002-3039-7462
author2_role author
author
dc.subject.none.fl_str_mv Colloids in medicine
Tissue engineering
Col·loides en medicina
Enginyeria de teixits
Àrees temàtiques de la UPC::Enginyeria biomèdica
topic Colloids in medicine
Tissue engineering
Col·loides en medicina
Enginyeria de teixits
Àrees temàtiques de la UPC::Enginyeria biomèdica
description Reactive Oxygen and Nitrogen Species (RONS) in biological systems display hormetic effects, capable of either promoting cell regenerative effects or inducing cell death. Recently, hydrogels have emerged as a promising delivery platform for RONS generated from Cold Atmospheric Plasmas (CAP), known as Plasma-Treated Hydrogels (PTH). PTH have been proposed as an alternative therapy to conventional cancer treatments, offering reduced side effects through the controlled and localized delivery of plasma-derived RONS. In this work, we have developed alginate-based PTH with dual therapeutic action provided by plasma-derived RONS acting as selective anticancer agents for osteosarcoma treatment, and biomolecules (hyaluronic acid and gelatin) to promote stem cell-mediated bone regeneration. For this purpose, we designed a novel manufacturing process to maximize the load of plasma-derived RONS within the PTH. Then, we assessed the PTH bioactivity on osteosarcoma MG-63 cells, and human mesenchymal stem cells (hMSCs). The results showed that the PTH composed of 0.25 % alginate +1 % hyaluronic acid is the most promising formulation in osteosarcoma treatment, showing a dual-action bioactivity as a selective cytotoxic anticancer agent, and as promoter of the proliferation and osteogenic differentiation of hMSCs. These findings provide strong evidence of the significant potential of PTH in the oncological field.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-02-01
2024
2024-05-28
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/408685
https://dx.doi.org/10.1016/j.ijbiomac.2023.128841
url https://hdl.handle.net/2117/408685
https://dx.doi.org/10.1016/j.ijbiomac.2023.128841
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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