Reelin expression is up-regulated in mice colon in response to acute colitis and provides resistance against colitis
Reelin is an extracellular matrix protein first known for its key role in neuronal migration. Studies in rodent small intestine suggested that reelin protects the organism from intestinal pathology. Here we determined in mice colon, by real time-PCR and immunological assays, the expression of the re...
| Autores: | , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2017 |
| País: | España |
| Institución: | Universidad de Sevilla (US) |
| Repositorio: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/85340 |
| Acceso en línea: | https://hdl.handle.net/11441/85340 https://doi.org/10.1016/j.bbadis.2016.11.028 |
| Access Level: | acceso abierto |
| Palabra clave: | Colon DNMT1 DSS-colitis Reelin |
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Reelin expression is up-regulated in mice colon in response to acute colitis and provides resistance against colitisCarvajal Vázquez, Ana EloisaVázquez Carretero, María DoloresGarcía Miranda, PabloPeral Rubio, María JoséCalonge Castrillo, María LuisaIlundáin Larrañeta, María Anunciación AnaColonDNMT1DSS-colitisReelinReelin is an extracellular matrix protein first known for its key role in neuronal migration. Studies in rodent small intestine suggested that reelin protects the organism from intestinal pathology. Here we determined in mice colon, by real time-PCR and immunological assays, the expression of the reelin signalling system; its response to dextran sulphate sodium (DSS) and the response of wild-type and reeler mice to DSS-treatment. DNA methylation was determined by bisulfite modification and sequencing of genomic DNA. In the colon mucosa reelin expression is restricted to the myofibroblasts, whereas both epithelial cells and myofibroblasts express reelin receptors (ApoER2 and VLDLR) and its effector protein Dab1. The muscle layer also expresses reelin. DSS-treatment reduces reelin expression in the muscle but it is activated in the mucosa. Activation of mucosal reelin is greater in magnitude and is delayed until after the activation of the myofibroblasts marker, α-SMA. This indicates that the DSS-induced reelin up-regulation results from changes in the reelin gene expression rather than from myofibroblasts proliferation. DSS-treatment does not modify Sp1 or Tbr1 mRNA abundance, but increases that of TGF-β1 and ApoER2, decreases that of CASK and DNMT1 and it also decreases the reelin promoter methylation. Finally, the reeler mice exhibit higher inflammatory scores than wild-type mice, indicating that the mutation increases the susceptibility to DSS-colitis. In summary, this data are the first to demonstrate that mouse distal colon increases reelin production in response to DSS-colitis via a DNMT1-dependent hypo-methylation of the gene promoter region and that reelin provides protection against colitisEspaña, Junta de Andalucía CTS 5884Elsevier2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/85340https://doi.org/10.1016/j.bbadis.2016.11.028reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésBiochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 1863 (2), 462-473.CTS 5884http://dx.doi.org/10.1016/j.bbadis.2016.11.028info:eu-repo/semantics/openAccessoai:idus.us.es:11441/853402026-06-17T12:51:07Z |
| dc.title.none.fl_str_mv |
Reelin expression is up-regulated in mice colon in response to acute colitis and provides resistance against colitis |
| title |
Reelin expression is up-regulated in mice colon in response to acute colitis and provides resistance against colitis |
| spellingShingle |
Reelin expression is up-regulated in mice colon in response to acute colitis and provides resistance against colitis Carvajal Vázquez, Ana Eloisa Colon DNMT1 DSS-colitis Reelin |
| title_short |
Reelin expression is up-regulated in mice colon in response to acute colitis and provides resistance against colitis |
| title_full |
Reelin expression is up-regulated in mice colon in response to acute colitis and provides resistance against colitis |
| title_fullStr |
Reelin expression is up-regulated in mice colon in response to acute colitis and provides resistance against colitis |
| title_full_unstemmed |
Reelin expression is up-regulated in mice colon in response to acute colitis and provides resistance against colitis |
| title_sort |
Reelin expression is up-regulated in mice colon in response to acute colitis and provides resistance against colitis |
| dc.creator.none.fl_str_mv |
Carvajal Vázquez, Ana Eloisa Vázquez Carretero, María Dolores García Miranda, Pablo Peral Rubio, María José Calonge Castrillo, María Luisa Ilundáin Larrañeta, María Anunciación Ana |
| author |
Carvajal Vázquez, Ana Eloisa |
| author_facet |
Carvajal Vázquez, Ana Eloisa Vázquez Carretero, María Dolores García Miranda, Pablo Peral Rubio, María José Calonge Castrillo, María Luisa Ilundáin Larrañeta, María Anunciación Ana |
| author_role |
author |
| author2 |
Vázquez Carretero, María Dolores García Miranda, Pablo Peral Rubio, María José Calonge Castrillo, María Luisa Ilundáin Larrañeta, María Anunciación Ana |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
Colon DNMT1 DSS-colitis Reelin |
| topic |
Colon DNMT1 DSS-colitis Reelin |
| description |
Reelin is an extracellular matrix protein first known for its key role in neuronal migration. Studies in rodent small intestine suggested that reelin protects the organism from intestinal pathology. Here we determined in mice colon, by real time-PCR and immunological assays, the expression of the reelin signalling system; its response to dextran sulphate sodium (DSS) and the response of wild-type and reeler mice to DSS-treatment. DNA methylation was determined by bisulfite modification and sequencing of genomic DNA. In the colon mucosa reelin expression is restricted to the myofibroblasts, whereas both epithelial cells and myofibroblasts express reelin receptors (ApoER2 and VLDLR) and its effector protein Dab1. The muscle layer also expresses reelin. DSS-treatment reduces reelin expression in the muscle but it is activated in the mucosa. Activation of mucosal reelin is greater in magnitude and is delayed until after the activation of the myofibroblasts marker, α-SMA. This indicates that the DSS-induced reelin up-regulation results from changes in the reelin gene expression rather than from myofibroblasts proliferation. DSS-treatment does not modify Sp1 or Tbr1 mRNA abundance, but increases that of TGF-β1 and ApoER2, decreases that of CASK and DNMT1 and it also decreases the reelin promoter methylation. Finally, the reeler mice exhibit higher inflammatory scores than wild-type mice, indicating that the mutation increases the susceptibility to DSS-colitis. In summary, this data are the first to demonstrate that mouse distal colon increases reelin production in response to DSS-colitis via a DNMT1-dependent hypo-methylation of the gene promoter region and that reelin provides protection against colitis |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion |
| format |
article |
| status_str |
acceptedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/11441/85340 https://doi.org/10.1016/j.bbadis.2016.11.028 |
| url |
https://hdl.handle.net/11441/85340 https://doi.org/10.1016/j.bbadis.2016.11.028 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 1863 (2), 462-473. CTS 5884 http://dx.doi.org/10.1016/j.bbadis.2016.11.028 |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf application/pdf |
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Elsevier |
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Elsevier |
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reponame:idUS. Depósito de Investigación de la Universidad de Sevilla instname:Universidad de Sevilla (US) |
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Universidad de Sevilla (US) |
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idUS. Depósito de Investigación de la Universidad de Sevilla |
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