Xanthine urolithiasis: Inhibitors of xanthine crystallization
Objective: To identify in vitro inhibitors of xanthine crystallization that have potential for inhibiting the formation of xanthine crystals in urine and preventing the development of the renal calculi in patients with xanthinuria. Methods: The formation of xanthine crystals in synthetic urine and t...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Instituto de Salud Carlos III (ISCIII) |
| Repositorio: | Repisalud |
| Idioma: | inglés |
| OAI Identifier: | oai:repisalud.isciii.es:20.500.12105/22606 |
| Acceso en línea: | https://hdl.handle.net/20.500.12105/22606 |
| Access Level: | acceso abierto |
| Palabra clave: | Xantina Xantinas Aldehído Oxidasa Regulación hacia Abajo Errores Innatos del Metabolismo Xantina Deshidrogenasa Cristalización Técnicas In Vitro Cálculos Renales Humanos Urolitiasis Errores Innatos del Metabolismo de la Purina-Pirimidina Precipitación Química Urolithiasis In Vitro Techniques Kidney Calculi Aldehyde Oxidase Humans Purine-Pyrimidine Metabolism, Inborn Errors Metabolism, Inborn Errors Crystallization Down-Regulation Xanthine Dehydrogenase Xanthines Chemical Precipitation Xanthine |
| Sumario: | Objective: To identify in vitro inhibitors of xanthine crystallization that have potential for inhibiting the formation of xanthine crystals in urine and preventing the development of the renal calculi in patients with xanthinuria. Methods: The formation of xanthine crystals in synthetic urine and the effects of 10 potential crystallization inhibitors were assessed using a kinetic turbidimetric system with a photometer. The maximum concentration tested for each compound was: 20 mg/L for 3-methylxanthine (3MX); 40 mg/L for 7-methylxanthine (7-MX), 1-methylxanthine (1-MX), theobromine (TB), theophylline, paraxanthine, and caffeine; 45 mg/L for 1-methyluric acid; 80 mg/L for 1,3-dimethyluric acid; and 200 mg/L for hypoxanthine. Scanning electron microscopy was used to examine the morphology of the crystals formed when inhibitory effects were observed. Results: Only 7-MX, 3-MX, and 1-MX significantly inhibited xanthine crystallization at the tested concentrations. Mixtures of inhibitors had an additive effect rather than a synergistic effect on crystallization. Conclusion: Two of the inhibitors identified here D 7-MX and 3-MX D are major metabolites of TB. In particular, after TB consumption, 20% is excreted in the urine as TB, 21.5% as 3-MX, and 36% as 7-MX. Thus, consumption of theobromine could protect patients with xanthinuria from the development of renal xanthine calculi. Clinical trials are necessary to demonstrate these effects in vivo. |
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