Characterization of the annual regulation of reproductive and immune parameters on the testis of European sea bass

The European sea bass, Dicentrarchus labrax L., is a seasonal gonochoristic species, which males are generally mature during the second year of life. It has been demonstrated that cytokines and immune cells play a key role in the testicular development. This reproductive-immune interaction might be...

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Autores: Valero, Yulema, Sánchez-Hernández, Miriam, García-Alcázar, Alicia, García-Ayala, Alfonsa, Cuesta, Alberto, Chaves-Pozo, Elena
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/313480
Acceso en línea:http://hdl.handle.net/10261/313480
Access Level:acceso abierto
Palabra clave:Acuicultura
Centro Oceanográfico de Murcia
European sea bass
Cell renewal
testis
hormone levels
Immune factors
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spelling Characterization of the annual regulation of reproductive and immune parameters on the testis of European sea bassValero, YulemaSánchez-Hernández, MiriamGarcía-Alcázar, AliciaGarcía-Ayala, AlfonsaCuesta, AlbertoChaves-Pozo, ElenaAcuiculturaCentro Oceanográfico de MurciaEuropean sea bassCell renewaltestishormone levelsImmune factorsThe European sea bass, Dicentrarchus labrax L., is a seasonal gonochoristic species, which males are generally mature during the second year of life. It has been demonstrated that cytokines and immune cells play a key role in the testicular development. This reproductive-immune interaction might be very important in the sea bass since several pathogens are able to colonize the gonad and persist in this tissue, altering further reproductive functions and spreading the disease. This study aims to investigate the reproductive cycle of one year European sea bass males by analyzing cell proliferation and apoptosis and expression profile of some reproductive and immune-related genes in the testis as well as the serum sex steroid levels. Our data demonstrate that in one year old European sea bass males, the testis undergoes the spermatogenesis process and that the reproductive and immune parameters analyzed varied during the reproductive cycle. In the testis, the highest proliferative rates were recorded at spermatogenesis stage, while the highest apoptotic rates were recorded at spawning stage. We have also analyzed, for the first time in European sea bass males, the serum levels of 17β-estradiol (E2) and dihydrotestosterone and the gene expression profile of the enzymes implied in their production, determining that at least E2 might be involved in the regulation of the reproductive cycle. Some immune relevant genes, including cytokines, lymphocyte receptors, anti-viral and antibacterial molecules were detected in the testis of naïve European sea bass specimens and their expression profile was related with the stages of the reproductive cycle, suggesting an important role for the defence of the reproductive tissues.SI202320232015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/313480reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésCentro Oceanográfico de Murciahttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=25896883info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3134802026-05-22T06:33:51Z
dc.title.none.fl_str_mv Characterization of the annual regulation of reproductive and immune parameters on the testis of European sea bass
title Characterization of the annual regulation of reproductive and immune parameters on the testis of European sea bass
spellingShingle Characterization of the annual regulation of reproductive and immune parameters on the testis of European sea bass
Valero, Yulema
Acuicultura
Centro Oceanográfico de Murcia
European sea bass
Cell renewal
testis
hormone levels
Immune factors
title_short Characterization of the annual regulation of reproductive and immune parameters on the testis of European sea bass
title_full Characterization of the annual regulation of reproductive and immune parameters on the testis of European sea bass
title_fullStr Characterization of the annual regulation of reproductive and immune parameters on the testis of European sea bass
title_full_unstemmed Characterization of the annual regulation of reproductive and immune parameters on the testis of European sea bass
title_sort Characterization of the annual regulation of reproductive and immune parameters on the testis of European sea bass
dc.creator.none.fl_str_mv Valero, Yulema
Sánchez-Hernández, Miriam
García-Alcázar, Alicia
García-Ayala, Alfonsa
Cuesta, Alberto
Chaves-Pozo, Elena
author Valero, Yulema
author_facet Valero, Yulema
Sánchez-Hernández, Miriam
García-Alcázar, Alicia
García-Ayala, Alfonsa
Cuesta, Alberto
Chaves-Pozo, Elena
author_role author
author2 Sánchez-Hernández, Miriam
García-Alcázar, Alicia
García-Ayala, Alfonsa
Cuesta, Alberto
Chaves-Pozo, Elena
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Acuicultura
Centro Oceanográfico de Murcia
European sea bass
Cell renewal
testis
hormone levels
Immune factors
topic Acuicultura
Centro Oceanográfico de Murcia
European sea bass
Cell renewal
testis
hormone levels
Immune factors
description The European sea bass, Dicentrarchus labrax L., is a seasonal gonochoristic species, which males are generally mature during the second year of life. It has been demonstrated that cytokines and immune cells play a key role in the testicular development. This reproductive-immune interaction might be very important in the sea bass since several pathogens are able to colonize the gonad and persist in this tissue, altering further reproductive functions and spreading the disease. This study aims to investigate the reproductive cycle of one year European sea bass males by analyzing cell proliferation and apoptosis and expression profile of some reproductive and immune-related genes in the testis as well as the serum sex steroid levels. Our data demonstrate that in one year old European sea bass males, the testis undergoes the spermatogenesis process and that the reproductive and immune parameters analyzed varied during the reproductive cycle. In the testis, the highest proliferative rates were recorded at spermatogenesis stage, while the highest apoptotic rates were recorded at spawning stage. We have also analyzed, for the first time in European sea bass males, the serum levels of 17β-estradiol (E2) and dihydrotestosterone and the gene expression profile of the enzymes implied in their production, determining that at least E2 might be involved in the regulation of the reproductive cycle. Some immune relevant genes, including cytokines, lymphocyte receptors, anti-viral and antibacterial molecules were detected in the testis of naïve European sea bass specimens and their expression profile was related with the stages of the reproductive cycle, suggesting an important role for the defence of the reproductive tissues.
publishDate 2015
dc.date.none.fl_str_mv 2015
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/313480
url http://hdl.handle.net/10261/313480
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Centro Oceanográfico de Murcia
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=25896883
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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