Development of rapid direct PCR assays to identify downy and powdery mildew and grey mould in Vitis vinifera tissues

Aims: The development of a rapid and reliable direct PCR method to detect fungal propagules in grapevine tissues without prior DNA purification steps, and illustration of its potential use with different examples.

Detalles Bibliográficos
Autores: Gindro, Katia, Lecoultre, Nicole, Molino, Luca, Joffrey, Jean-Pierre, Schnee, Sylvain, Voinesco, Francine, Alonso-Villaverde Iglesias, Virginia, Viret, Olivier, Dubuis, Pierre-Henri
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/110804
Acceso en línea:http://hdl.handle.net/10261/110804
Access Level:acceso abierto
Palabra clave:Plasmopara viticola
Botrytis cinerea
Electron microscopy
Direct PCR
Descripción
Sumario:Aims: The development of a rapid and reliable direct PCR method to detect fungal propagules in grapevine tissues without prior DNA purification steps, and illustration of its potential use with different examples.