Gene Expression Analysis of Plum pox virus (Sharka) Susceptibility/Resistance in Apricot ( Prunus armeniaca L.)

RNA-Seq has proven to be a very powerful tool in the analysis of the Plum pox virus (PPV, sharka disease)/Prunus interaction. This technique is an important complementary tool to other means of studying genomics. In this work an analysis of gene expression of resistance/susceptibility to PPV in apri...

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Autores: Rubio, Manuel, Ballester Frutos, Ana Rosa, Olivares, Pedro M., Castro de Moura, Manuel, Dicenta, Federico, Martínez-Gómez, Pedro
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/127780
Acceso en línea:http://hdl.handle.net/10261/127780
Access Level:acceso abierto
Palabra clave:RNA-Seq
Plum pox virus
Pathogen response
Infection
Resistence
Genomics
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spelling Gene Expression Analysis of Plum pox virus (Sharka) Susceptibility/Resistance in Apricot ( Prunus armeniaca L.)Rubio, ManuelBallester Frutos, Ana RosaOlivares, Pedro M.Castro de Moura, ManuelDicenta, FedericoMartínez-Gómez, PedroRNA-SeqPlum pox virusPathogen responseInfectionResistenceGenomicsRNA-Seq has proven to be a very powerful tool in the analysis of the Plum pox virus (PPV, sharka disease)/Prunus interaction. This technique is an important complementary tool to other means of studying genomics. In this work an analysis of gene expression of resistance/susceptibility to PPV in apricot is performed. RNA-Seq has been applied to analyse the gene expression changes induced by PPV infection in leaves from two full-sib apricot genotypes, “Rojo Pasión” and “Z506-7”, resistant and susceptible to PPV, respectively. Transcriptomic analyses revealed the existence of more than 2,000 genes related to the pathogen response and resistance to PPV in apricot. These results showed that the response to infection by the virus in the susceptible genotype is associated with an induction of genes involved in pathogen resistance such as the allene oxide synthase, S-adenosylmethionine synthetase 2 and the major MLP-like protein 423. Over-expression of the Dicer protein 2a may indicate the suppression of a gene silencing mechanism of the plant by PPV HCPro and P1 PPV proteins. On the other hand, there were 164 genes involved in resistance mechanisms that have been identified in apricot, 49 of which are located in the PPVres region (scaffold 1 positions from 8,050,804 to 8,244,925), which is responsible for PPV resistance in apricot. Among these genes in apricot there are several MATH domain-containing genes, although other genes inside (Pleiotropic drug resistance 9 gene) or outside (CAP, Cysteine-rich secretory proteins, Antigen 5 and Pathogenesis-related 1 protein; and LEA, Late embryogenesis abundant protein) PPVres region could also be involved in the resistance.This study was supported by the projects “Gene expression analysis of the resistance to Plum pox virus, PPV (sharka) in apricot by transcriptome deep sequencing (RNA-Seq)” (AGL2010-16335) and “Regulation of gene expression in the resistance to Plum pox virus (Sharka) induced in peach by ‘Garrigues’ almond grafting” (AGL2013-43550-R) of the Spanish Ministry of Economy and Competitiveness. A.R. Ballester and M. Rubio are grateful to CSIC and the European Social Fund for their postdoctoral contracts.Peer reviewedPublic Library of ScienceMinisterio de Economía y Competitividad (España)European CommissionConsejo Superior de Investigaciones Científicas (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201620162015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/127780reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-43550-Rhttp://dx.doi.org/10.1371/journal.pone.0144670Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1277802026-05-22T06:33:51Z
dc.title.none.fl_str_mv Gene Expression Analysis of Plum pox virus (Sharka) Susceptibility/Resistance in Apricot ( Prunus armeniaca L.)
title Gene Expression Analysis of Plum pox virus (Sharka) Susceptibility/Resistance in Apricot ( Prunus armeniaca L.)
spellingShingle Gene Expression Analysis of Plum pox virus (Sharka) Susceptibility/Resistance in Apricot ( Prunus armeniaca L.)
Rubio, Manuel
RNA-Seq
Plum pox virus
Pathogen response
Infection
Resistence
Genomics
title_short Gene Expression Analysis of Plum pox virus (Sharka) Susceptibility/Resistance in Apricot ( Prunus armeniaca L.)
title_full Gene Expression Analysis of Plum pox virus (Sharka) Susceptibility/Resistance in Apricot ( Prunus armeniaca L.)
title_fullStr Gene Expression Analysis of Plum pox virus (Sharka) Susceptibility/Resistance in Apricot ( Prunus armeniaca L.)
title_full_unstemmed Gene Expression Analysis of Plum pox virus (Sharka) Susceptibility/Resistance in Apricot ( Prunus armeniaca L.)
title_sort Gene Expression Analysis of Plum pox virus (Sharka) Susceptibility/Resistance in Apricot ( Prunus armeniaca L.)
dc.creator.none.fl_str_mv Rubio, Manuel
Ballester Frutos, Ana Rosa
Olivares, Pedro M.
Castro de Moura, Manuel
Dicenta, Federico
Martínez-Gómez, Pedro
author Rubio, Manuel
author_facet Rubio, Manuel
Ballester Frutos, Ana Rosa
Olivares, Pedro M.
Castro de Moura, Manuel
Dicenta, Federico
Martínez-Gómez, Pedro
author_role author
author2 Ballester Frutos, Ana Rosa
Olivares, Pedro M.
Castro de Moura, Manuel
Dicenta, Federico
Martínez-Gómez, Pedro
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Commission
Consejo Superior de Investigaciones Científicas (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv RNA-Seq
Plum pox virus
Pathogen response
Infection
Resistence
Genomics
topic RNA-Seq
Plum pox virus
Pathogen response
Infection
Resistence
Genomics
description RNA-Seq has proven to be a very powerful tool in the analysis of the Plum pox virus (PPV, sharka disease)/Prunus interaction. This technique is an important complementary tool to other means of studying genomics. In this work an analysis of gene expression of resistance/susceptibility to PPV in apricot is performed. RNA-Seq has been applied to analyse the gene expression changes induced by PPV infection in leaves from two full-sib apricot genotypes, “Rojo Pasión” and “Z506-7”, resistant and susceptible to PPV, respectively. Transcriptomic analyses revealed the existence of more than 2,000 genes related to the pathogen response and resistance to PPV in apricot. These results showed that the response to infection by the virus in the susceptible genotype is associated with an induction of genes involved in pathogen resistance such as the allene oxide synthase, S-adenosylmethionine synthetase 2 and the major MLP-like protein 423. Over-expression of the Dicer protein 2a may indicate the suppression of a gene silencing mechanism of the plant by PPV HCPro and P1 PPV proteins. On the other hand, there were 164 genes involved in resistance mechanisms that have been identified in apricot, 49 of which are located in the PPVres region (scaffold 1 positions from 8,050,804 to 8,244,925), which is responsible for PPV resistance in apricot. Among these genes in apricot there are several MATH domain-containing genes, although other genes inside (Pleiotropic drug resistance 9 gene) or outside (CAP, Cysteine-rich secretory proteins, Antigen 5 and Pathogenesis-related 1 protein; and LEA, Late embryogenesis abundant protein) PPVres region could also be involved in the resistance.
publishDate 2015
dc.date.none.fl_str_mv 2015
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/127780
url http://hdl.handle.net/10261/127780
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-43550-R
http://dx.doi.org/10.1371/journal.pone.0144670
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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