CLytA-DAAO, free and immobilized in magnetic nanoparticles, induces cell death in human cancer cells

D-amino acid oxidase (DAAO) catalyzes the oxidation of D-amino acids generating hydrogen peroxide, a potential producer of reactive oxygen species. In this study, we used a CLytA-DAAO chimera, both free and bound to magnetic nanoparticles, against colon carcinoma, pancreatic adenocarcinoma, and glio...

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Autores: Fuentes-Baile, María, Bello-Gil, Daniel, Pérez-Valenciano, Elizabeth, Sanz, Jesús, García-Morales, Pilar, Maestro García-Donas, María Beatriz, Ventero, María, Alenda, Cristina, Barberá, Victor, Saceda, Manuel
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/94062
Acceso en línea:https://hdl.handle.net/20.500.14352/94062
Access Level:acceso abierto
Palabra clave:Biología molecular (Biología)
2403 Bioquímica
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spelling CLytA-DAAO, free and immobilized in magnetic nanoparticles, induces cell death in human cancer cellsFuentes-Baile, MaríaBello-Gil, DanielPérez-Valenciano, ElizabethSanz, JesúsGarcía-Morales, PilarMaestro García-Donas, María BeatrizVentero, MaríaAlenda, CristinaBarberá, VictorSaceda, ManuelBiología molecular (Biología)2403 BioquímicaD-amino acid oxidase (DAAO) catalyzes the oxidation of D-amino acids generating hydrogen peroxide, a potential producer of reactive oxygen species. In this study, we used a CLytA-DAAO chimera, both free and bound to magnetic nanoparticles, against colon carcinoma, pancreatic adenocarcinoma, and glioblastoma cell lines. We found that the enzyme induces cell death in most of the cell lines tested and its efficiency increases significantly when it is immobilized in nanoparticles. We also tested this enzyme therapy in non-tumor cells, and we found that there is not cell death induction, or it is significantly lower than in tumor cells. The mechanism triggering cell death is apparently a classical apoptosis pathway in the glioblastoma cell lines, while in colon and pancreatic carcinoma cell lines, CLytA-DAAO-induced cell death is a necrosis. Our results constitute a proof of concept that an enzymatic therapy, based on magnetic nanoparticles-delivering CLytA-DAAO, could constitute a useful therapy against cancer and besides it could be used as an enhancer of other treatments such as epigenetic therapy, radiotherapy, and treatments based on DNA repair.Universidad Complutense de Madrid20202020-01-0120202020-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/94062reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/940622026-06-02T12:44:21Z
dc.title.none.fl_str_mv CLytA-DAAO, free and immobilized in magnetic nanoparticles, induces cell death in human cancer cells
title CLytA-DAAO, free and immobilized in magnetic nanoparticles, induces cell death in human cancer cells
spellingShingle CLytA-DAAO, free and immobilized in magnetic nanoparticles, induces cell death in human cancer cells
Fuentes-Baile, María
Biología molecular (Biología)
2403 Bioquímica
title_short CLytA-DAAO, free and immobilized in magnetic nanoparticles, induces cell death in human cancer cells
title_full CLytA-DAAO, free and immobilized in magnetic nanoparticles, induces cell death in human cancer cells
title_fullStr CLytA-DAAO, free and immobilized in magnetic nanoparticles, induces cell death in human cancer cells
title_full_unstemmed CLytA-DAAO, free and immobilized in magnetic nanoparticles, induces cell death in human cancer cells
title_sort CLytA-DAAO, free and immobilized in magnetic nanoparticles, induces cell death in human cancer cells
dc.creator.none.fl_str_mv Fuentes-Baile, María
Bello-Gil, Daniel
Pérez-Valenciano, Elizabeth
Sanz, Jesús
García-Morales, Pilar
Maestro García-Donas, María Beatriz
Ventero, María
Alenda, Cristina
Barberá, Victor
Saceda, Manuel
author Fuentes-Baile, María
author_facet Fuentes-Baile, María
Bello-Gil, Daniel
Pérez-Valenciano, Elizabeth
Sanz, Jesús
García-Morales, Pilar
Maestro García-Donas, María Beatriz
Ventero, María
Alenda, Cristina
Barberá, Victor
Saceda, Manuel
author_role author
author2 Bello-Gil, Daniel
Pérez-Valenciano, Elizabeth
Sanz, Jesús
García-Morales, Pilar
Maestro García-Donas, María Beatriz
Ventero, María
Alenda, Cristina
Barberá, Victor
Saceda, Manuel
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv Biología molecular (Biología)
2403 Bioquímica
topic Biología molecular (Biología)
2403 Bioquímica
description D-amino acid oxidase (DAAO) catalyzes the oxidation of D-amino acids generating hydrogen peroxide, a potential producer of reactive oxygen species. In this study, we used a CLytA-DAAO chimera, both free and bound to magnetic nanoparticles, against colon carcinoma, pancreatic adenocarcinoma, and glioblastoma cell lines. We found that the enzyme induces cell death in most of the cell lines tested and its efficiency increases significantly when it is immobilized in nanoparticles. We also tested this enzyme therapy in non-tumor cells, and we found that there is not cell death induction, or it is significantly lower than in tumor cells. The mechanism triggering cell death is apparently a classical apoptosis pathway in the glioblastoma cell lines, while in colon and pancreatic carcinoma cell lines, CLytA-DAAO-induced cell death is a necrosis. Our results constitute a proof of concept that an enzymatic therapy, based on magnetic nanoparticles-delivering CLytA-DAAO, could constitute a useful therapy against cancer and besides it could be used as an enhancer of other treatments such as epigenetic therapy, radiotherapy, and treatments based on DNA repair.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01
2020
2020-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/94062
url https://hdl.handle.net/20.500.14352/94062
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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