Simultaneous extraction of rosemary and spinach leaves and its effect on the antioxidant activity of products

Mixed vegetal extracts are interesting target of new products as nutraceuticals, superior ingredients for the design of functional food, singular ingredients for cosmetics, etc. In this work the extraction of a mixture of spinach and rosemary leaves (50 wt.% of each plant) was investigated in terms...

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Autores: Vázquez, Erika, García-Risco, Mónica R., Jaime, Laura, Reglero, Guillermo, Fornari, Tiziana
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2013
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/100308
Acceso en línea:http://hdl.handle.net/10261/100308
Access Level:acceso abierto
Palabra clave:Rosemary
Spinach
Carotenoids
Phenolic diterpenes
Antioxidants
Pressurized liquid extraction
Supercritical fluid extraction
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spelling Simultaneous extraction of rosemary and spinach leaves and its effect on the antioxidant activity of productsVázquez, ErikaGarcía-Risco, Mónica R.Jaime, LauraReglero, GuillermoFornari, TizianaRosemarySpinachCarotenoidsPhenolic diterpenesAntioxidantsPressurized liquid extractionSupercritical fluid extractionMixed vegetal extracts are interesting target of new products as nutraceuticals, superior ingredients for the design of functional food, singular ingredients for cosmetics, etc. In this work the extraction of a mixture of spinach and rosemary leaves (50 wt.% of each plant) was investigated in terms of its antioxidant activity, and compared with the extraction of the separate species. Phenolic diterpenes of rosemary and carotenoids of spinach were target compounds due their recognized biological activities. Two different extraction techniques were applied, namely pressurized liquid extraction using hexane at two different temperatures (100 and 150 C) and supercritical fluid extraction with pure carbon dioxide at 40 C and two different pressures (20 and 30 MPa). For each extraction technique and conditions three different raw materials were employed: spinach leaves, rosemary leaves and the mixture 50:50 of spinach and rosemary leaves. The antioxidant activity of the samples produced was evaluated with the ABTS assay and showed to be enhanced when the species are simultaneously extracted, with antioxidant values around 20% higher than the values corresponding to mixing the extracts obtained by separate. A possible synergic effect between carotenoids and phenolic diterpenes was studied, although no specific synergic activity could be observed. However, the enhanced antioxidant activity could be attributed to a definite increase of the concentration of carnosic acid, which was observed in the samples produced by the simultaneous extraction. © 2013 Elsevier B.V. All rights reserved.This work has been supported by project INNSAMED IPT-300000-2010-34 (subprogram INNPACTO) from Ministry of Science and Innovation (Spain) and project ALIBIRD-S2009/AGR-1469 from Autonomous Community of Madrid.Peer ReviewedElsevierComunidad de MadridMinisterio de Ciencia e Innovación (España)2014201420132014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/100308reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#S2009/AGR-1469/ALIBIRDhttp://dx.doi.org/10.1016/j.supflu.2013.07.004info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1003082026-05-22T06:33:51Z
dc.title.none.fl_str_mv Simultaneous extraction of rosemary and spinach leaves and its effect on the antioxidant activity of products
title Simultaneous extraction of rosemary and spinach leaves and its effect on the antioxidant activity of products
spellingShingle Simultaneous extraction of rosemary and spinach leaves and its effect on the antioxidant activity of products
Vázquez, Erika
Rosemary
Spinach
Carotenoids
Phenolic diterpenes
Antioxidants
Pressurized liquid extraction
Supercritical fluid extraction
title_short Simultaneous extraction of rosemary and spinach leaves and its effect on the antioxidant activity of products
title_full Simultaneous extraction of rosemary and spinach leaves and its effect on the antioxidant activity of products
title_fullStr Simultaneous extraction of rosemary and spinach leaves and its effect on the antioxidant activity of products
title_full_unstemmed Simultaneous extraction of rosemary and spinach leaves and its effect on the antioxidant activity of products
title_sort Simultaneous extraction of rosemary and spinach leaves and its effect on the antioxidant activity of products
dc.creator.none.fl_str_mv Vázquez, Erika
García-Risco, Mónica R.
Jaime, Laura
Reglero, Guillermo
Fornari, Tiziana
author Vázquez, Erika
author_facet Vázquez, Erika
García-Risco, Mónica R.
Jaime, Laura
Reglero, Guillermo
Fornari, Tiziana
author_role author
author2 García-Risco, Mónica R.
Jaime, Laura
Reglero, Guillermo
Fornari, Tiziana
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Comunidad de Madrid
Ministerio de Ciencia e Innovación (España)
dc.subject.none.fl_str_mv Rosemary
Spinach
Carotenoids
Phenolic diterpenes
Antioxidants
Pressurized liquid extraction
Supercritical fluid extraction
topic Rosemary
Spinach
Carotenoids
Phenolic diterpenes
Antioxidants
Pressurized liquid extraction
Supercritical fluid extraction
description Mixed vegetal extracts are interesting target of new products as nutraceuticals, superior ingredients for the design of functional food, singular ingredients for cosmetics, etc. In this work the extraction of a mixture of spinach and rosemary leaves (50 wt.% of each plant) was investigated in terms of its antioxidant activity, and compared with the extraction of the separate species. Phenolic diterpenes of rosemary and carotenoids of spinach were target compounds due their recognized biological activities. Two different extraction techniques were applied, namely pressurized liquid extraction using hexane at two different temperatures (100 and 150 C) and supercritical fluid extraction with pure carbon dioxide at 40 C and two different pressures (20 and 30 MPa). For each extraction technique and conditions three different raw materials were employed: spinach leaves, rosemary leaves and the mixture 50:50 of spinach and rosemary leaves. The antioxidant activity of the samples produced was evaluated with the ABTS assay and showed to be enhanced when the species are simultaneously extracted, with antioxidant values around 20% higher than the values corresponding to mixing the extracts obtained by separate. A possible synergic effect between carotenoids and phenolic diterpenes was studied, although no specific synergic activity could be observed. However, the enhanced antioxidant activity could be attributed to a definite increase of the concentration of carnosic acid, which was observed in the samples produced by the simultaneous extraction. © 2013 Elsevier B.V. All rights reserved.
publishDate 2013
dc.date.none.fl_str_mv 2013
2014
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/100308
url http://hdl.handle.net/10261/100308
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
S2009/AGR-1469/ALIBIRD
http://dx.doi.org/10.1016/j.supflu.2013.07.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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