RNA-seq analysis and gene expression dynamics in the salivary glands of the argasid tick Ornithodoros erraticus along the trophogonic cycle

24 páginas, 6 tablas, 6 figuras

Detalles Bibliográficos
Autores: Pérez Sánchez, Ricardo, Carnero Morán, Ángel, Soriano, Beatriz, Llorens, Carlos, Oleaga, Ana
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/236699
Acceso en línea:http://hdl.handle.net/10261/236699
Access Level:acceso abierto
Palabra clave:Ornithodoros erraticus
Soft ticks
Salivary glands
Transcriptomes
Diferential gene expression
Vaccinomics
Vaccines
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spelling RNA-seq analysis and gene expression dynamics in the salivary glands of the argasid tick Ornithodoros erraticus along the trophogonic cyclePérez Sánchez, RicardoCarnero Morán, ÁngelSoriano, BeatrizLlorens, CarlosOleaga, AnaOrnithodoros erraticusSoft ticksSalivary glandsTranscriptomesDiferential gene expressionVaccinomicsVaccines24 páginas, 6 tablas, 6 figurasBackground: The argasid tick Ornithodoros erraticus is the main vector of tick-borne human relapsing fever (TBRF) and African swine fever (ASF) in the Mediterranean Basin. Tick salivary proteins secreted to the host at the feeding interface play critical roles for tick feeding and may contribute to host infection by tick-borne pathogens; accord‑ingly, these proteins represent interesting antigen targets for the development of vaccines aimed at the control and prevention of tick infestations and tick-borne diseases.Methods: To identify these proteins, the transcriptome of the salivary glands of O. erraticus was de novo assembled and the salivary gene expression dynamics assessed throughout the trophogonic cycle using Illumina sequencing. The genes diferentially upregulated after feeding were selected and discussed as potential antigen candidates for tick vaccines.Results: Transcriptome assembly resulted in 22,007 transcripts and 18,961 annotated transcripts, which represent 86.15% of annotation success. Most salivary gene expression took place during the frst 7 days after feeding (2088 upregulated transcripts), while only a few genes (122 upregulated transcripts) were diferentially expressed from day 7 post-feeding onwards. The protein families more abundantly overrepresented after feeding were lipocalins, acid and basic tail proteins, proteases (particularly metalloproteases), protease inhibitors, secreted phospholipases A2, 5′-nucle‑otidases/apyrases and heme-binding vitellogenin-like proteins. All of them are functionally related to blood ingestion and regulation of host defensive responses, so they can be interesting candidate protective antigens for vaccines.Conclusions: The O. erraticus sialotranscriptome contains thousands of protein coding sequences—many of them belonging to large conserved multigene protein families—and shows a complexity and functional redundancy similar to those observed in the sialomes of other argasid and ixodid tick species. This high functional redundancy emphasises the need for developing multiantigenic tick vaccines to reach full protection. This research provides a set of promis‑ ing candidate antigens for the development of vaccines for the control of O. erraticus infestations and prevention of tick-borne diseases of public and veterinary health relevance, such as TBRF and ASF. Additionally, this transcriptome constitutes a valuable reference database for proteomics studies of the saliva and salivary glands of O. erraticus.This research was funded by project RTI2018-098297-B-I00, granted by the Spanish Ministry of Science, Innovation and UniversitiesPeer reviewedBioMed CentralMinisterio de Ciencia, Innovación y Universidades (España)Pérez Sánchez, Ricardo [0000-0001-8875-439X]Carnero-Morán, Ángel [0000-0001-5292-3489]Oleaga, Ana [0000-0002-8019-7354]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/236699reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-098297-B-I00http://dx.doi.org/10.1186/s13071-021-04671-zSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2366992026-05-22T06:33:51Z
dc.title.none.fl_str_mv RNA-seq analysis and gene expression dynamics in the salivary glands of the argasid tick Ornithodoros erraticus along the trophogonic cycle
title RNA-seq analysis and gene expression dynamics in the salivary glands of the argasid tick Ornithodoros erraticus along the trophogonic cycle
spellingShingle RNA-seq analysis and gene expression dynamics in the salivary glands of the argasid tick Ornithodoros erraticus along the trophogonic cycle
Pérez Sánchez, Ricardo
Ornithodoros erraticus
Soft ticks
Salivary glands
Transcriptomes
Diferential gene expression
Vaccinomics
Vaccines
title_short RNA-seq analysis and gene expression dynamics in the salivary glands of the argasid tick Ornithodoros erraticus along the trophogonic cycle
title_full RNA-seq analysis and gene expression dynamics in the salivary glands of the argasid tick Ornithodoros erraticus along the trophogonic cycle
title_fullStr RNA-seq analysis and gene expression dynamics in the salivary glands of the argasid tick Ornithodoros erraticus along the trophogonic cycle
title_full_unstemmed RNA-seq analysis and gene expression dynamics in the salivary glands of the argasid tick Ornithodoros erraticus along the trophogonic cycle
title_sort RNA-seq analysis and gene expression dynamics in the salivary glands of the argasid tick Ornithodoros erraticus along the trophogonic cycle
dc.creator.none.fl_str_mv Pérez Sánchez, Ricardo
Carnero Morán, Ángel
Soriano, Beatriz
Llorens, Carlos
Oleaga, Ana
author Pérez Sánchez, Ricardo
author_facet Pérez Sánchez, Ricardo
Carnero Morán, Ángel
Soriano, Beatriz
Llorens, Carlos
Oleaga, Ana
author_role author
author2 Carnero Morán, Ángel
Soriano, Beatriz
Llorens, Carlos
Oleaga, Ana
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Pérez Sánchez, Ricardo [0000-0001-8875-439X]
Carnero-Morán, Ángel [0000-0001-5292-3489]
Oleaga, Ana [0000-0002-8019-7354]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Ornithodoros erraticus
Soft ticks
Salivary glands
Transcriptomes
Diferential gene expression
Vaccinomics
Vaccines
topic Ornithodoros erraticus
Soft ticks
Salivary glands
Transcriptomes
Diferential gene expression
Vaccinomics
Vaccines
description 24 páginas, 6 tablas, 6 figuras
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/236699
url http://hdl.handle.net/10261/236699
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-098297-B-I00
http://dx.doi.org/10.1186/s13071-021-04671-z
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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