Efficient Translation of Pelargonium line pattern virus RNAs Relies on a TED-Like 3 &apos

[EN] Cap-independent translational enhancers (CITEs) have been identified at the 3'-terminal regions of distinct plant positive-strand RNA viruses belonging to families Tombusviridae and Luteoviridae. On the bases of their structural and/or functional requirements, at least six classes of C...

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Autores: Blanco Pérez, Marta, Ruiz, Leticia, Perez-Cañamas, M, HERNANDEZ FORT, CARMEN
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/83590
Acceso en línea:https://riunet.upv.es/handle/10251/83590
Access Level:acceso abierto
Palabra clave:CAP independent translation
Tobacco necrosis virus
Plant viral RNAs
Satellite tobacco
Messenger-RNA
Untranslated regions
Family Tombusviridae
Genome organization
Loop interactions
Stem loop
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oai_identifier_str oai:riunet.upv.es:10251/83590
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network_name_str España
repository_id_str
dc.title.none.fl_str_mv Efficient Translation of Pelargonium line pattern virus RNAs Relies on a TED-Like 3 &apos
-Translational Enhancer that Communicates with the Corresponding 5 &apos
-Region through a Long-Distance RNA-RNA Interaction
title Efficient Translation of Pelargonium line pattern virus RNAs Relies on a TED-Like 3 &apos
spellingShingle Efficient Translation of Pelargonium line pattern virus RNAs Relies on a TED-Like 3 &apos
Blanco Pérez, Marta
CAP independent translation
Tobacco necrosis virus
Plant viral RNAs
Satellite tobacco
Messenger-RNA
Untranslated regions
Family Tombusviridae
Genome organization
Loop interactions
Stem loop
title_short Efficient Translation of Pelargonium line pattern virus RNAs Relies on a TED-Like 3 &apos
title_full Efficient Translation of Pelargonium line pattern virus RNAs Relies on a TED-Like 3 &apos
title_fullStr Efficient Translation of Pelargonium line pattern virus RNAs Relies on a TED-Like 3 &apos
title_full_unstemmed Efficient Translation of Pelargonium line pattern virus RNAs Relies on a TED-Like 3 &apos
title_sort Efficient Translation of Pelargonium line pattern virus RNAs Relies on a TED-Like 3 &apos
dc.creator.none.fl_str_mv Blanco Pérez, Marta
Ruiz, Leticia
Perez-Cañamas, M
HERNANDEZ FORT, CARMEN
author Blanco Pérez, Marta
author_facet Blanco Pérez, Marta
Ruiz, Leticia
Perez-Cañamas, M
HERNANDEZ FORT, CARMEN
author_role author
author2 Ruiz, Leticia
Perez-Cañamas, M
HERNANDEZ FORT, CARMEN
author2_role author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
Ministerio de Ciencia e Innovación
Ministerio de Economía y Competitividad
Generalitat Valenciana
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv CAP independent translation
Tobacco necrosis virus
Plant viral RNAs
Satellite tobacco
Messenger-RNA
Untranslated regions
Family Tombusviridae
Genome organization
Loop interactions
Stem loop
topic CAP independent translation
Tobacco necrosis virus
Plant viral RNAs
Satellite tobacco
Messenger-RNA
Untranslated regions
Family Tombusviridae
Genome organization
Loop interactions
Stem loop
description [EN] Cap-independent translational enhancers (CITEs) have been identified at the 3'-terminal regions of distinct plant positive-strand RNA viruses belonging to families Tombusviridae and Luteoviridae. On the bases of their structural and/or functional requirements, at least six classes of CITEs have been defined whose distribution does not correlate with taxonomy. The so-called TED class has been relatively under-studied and its functionality only confirmed in the case of Satellite tobacco necrosis virus, a parasitic subviral agent. The 3' untranslated region of the monopartite genome of Pelargonium line pattern virus (PLPV), the recommended type member of a tentative new genus (Pelarspovirus) in the family Tombusviridae, was predicted to contain a TED-like CITE. Similar CITEs can be anticipated in some other related viruses though none has been experimentally verified. Here, in the first place, we have performed a reassessment of the structure of the putative PLPV-TED through in silico predictions and in vitro SHAPE analysis with the full-length PLPV genome, which has indicated that the presumed TED element is larger than previously proposed. The extended conformation of the TED is strongly supported by the pattern of natural sequence variation, thus providing comparative structural evidence in support of the structural data obtained by in silico and in vitro approaches. Next, we have obtained experimental evidence demonstrating the in vivo activity of the PLPV-TED in the genomic (g) RNA, and also in the subgenomic (sg) RNA that the virus produces to express 3'-proximal genes. Besides other structural features, the results have highlighted the key role of long-distance kissing-loop interactions between the 3'-CITE and 5'-proximal hairpins for gRNA and sgRNA translation. Bioassays of CITE mutants have confirmed the importance of the identified 5'-3' RNA communication for viral infectivity and, moreover, have underlined the strong evolutionary constraints that may operate on genome stretches with both regulatory and coding functions.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-04-04
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/83590
url https://riunet.upv.es/handle/10251/83590
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BFU2009-11699 Estudio De Mecanismos De Traduccion No Canonicos En Virus De Plantas: Analisis De Las Posibles Ventajas De Su Utilizacion Frente Al Mecanismo Convencional
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BFU2012-36095 ANALISIS DE UNA RELACION COMENSALISTA VIRUS-PLANTA: ESTUDIO DE DETERMINANTES DE ACUMULACION VIRAL Y DE POSIBLES ALTERACIONES EPIGENETICAS EN EL GENOMA DEL HUESPED
Generalitat Valenciana https://doi.org/10.13039/501100003359 ACOMP%2F2012%2F100
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Efficient Translation of Pelargonium line pattern virus RNAs Relies on a TED-Like 3 &apos-Translational Enhancer that Communicates with the Corresponding 5 &apos-Region through a Long-Distance RNA-RNA InteractionBlanco Pérez, MartaRuiz, LeticiaPerez-Cañamas, MHERNANDEZ FORT, CARMENCAP independent translationTobacco necrosis virusPlant viral RNAsSatellite tobaccoMessenger-RNAUntranslated regionsFamily TombusviridaeGenome organizationLoop interactionsStem loop[EN] Cap-independent translational enhancers (CITEs) have been identified at the 3'-terminal regions of distinct plant positive-strand RNA viruses belonging to families Tombusviridae and Luteoviridae. On the bases of their structural and/or functional requirements, at least six classes of CITEs have been defined whose distribution does not correlate with taxonomy. The so-called TED class has been relatively under-studied and its functionality only confirmed in the case of Satellite tobacco necrosis virus, a parasitic subviral agent. The 3' untranslated region of the monopartite genome of Pelargonium line pattern virus (PLPV), the recommended type member of a tentative new genus (Pelarspovirus) in the family Tombusviridae, was predicted to contain a TED-like CITE. Similar CITEs can be anticipated in some other related viruses though none has been experimentally verified. Here, in the first place, we have performed a reassessment of the structure of the putative PLPV-TED through in silico predictions and in vitro SHAPE analysis with the full-length PLPV genome, which has indicated that the presumed TED element is larger than previously proposed. The extended conformation of the TED is strongly supported by the pattern of natural sequence variation, thus providing comparative structural evidence in support of the structural data obtained by in silico and in vitro approaches. Next, we have obtained experimental evidence demonstrating the in vivo activity of the PLPV-TED in the genomic (g) RNA, and also in the subgenomic (sg) RNA that the virus produces to express 3'-proximal genes. Besides other structural features, the results have highlighted the key role of long-distance kissing-loop interactions between the 3'-CITE and 5'-proximal hairpins for gRNA and sgRNA translation. Bioassays of CITE mutants have confirmed the importance of the identified 5'-3' RNA communication for viral infectivity and, moreover, have underlined the strong evolutionary constraints that may operate on genome stretches with both regulatory and coding functions.This work was supported by grants BFU2009-11699 and BFU2012-36095 from the Ministerio de Investigacion, Ciencia e Innovacion (MICINN, Spain, www.micinn.es) and the Ministerio de Economia y Competitividad (MINECO, Spain, http://www.mineco.gob.es), respectively, and ACOMP/2012/100 from the Generalitat Valenciana (http://www.gva.es) (to C.H.). MBP and LR were the recipients of a predoctoral and postdoctoral (Juan de la Cierva program) contract, respectively, from MICINN, and MPC was the recipient of a predoctoral contract from MINECO.Public Library of ScienceInstituto Universitario Mixto de Biología Molecular y Celular de PlantasMinisterio de Ciencia e InnovaciónMinisterio de Economía y CompetitividadGeneralitat ValencianaRepositorio Institucional de la Universitat Politècnica de València Riunet20162016-04-04journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/83590reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BFU2009-11699 Estudio De Mecanismos De Traduccion No Canonicos En Virus De Plantas: Analisis De Las Posibles Ventajas De Su Utilizacion Frente Al Mecanismo ConvencionalMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BFU2012-36095 ANALISIS DE UNA RELACION COMENSALISTA VIRUS-PLANTA: ESTUDIO DE DETERMINANTES DE ACUMULACION VIRAL Y DE POSIBLES ALTERACIONES EPIGENETICAS EN EL GENOMA DEL HUESPEDGeneralitat Valenciana https://doi.org/10.13039/501100003359 ACOMP%2F2012%2F100open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/835902026-06-13T07:49:27Z
score 15,300724

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