Analysis and application of a suite of recombinant endo-β(1,3)-d-glucanases for studying fungal cell walls
[EN]The fungal cell wall is an essential and robust external structure that protects the cell from the environment. It is mainly composed of polysaccharides with different functions, some of which are necessary for cell integrity. Thus, the process of fractionation and analysis of cell wall polysacc...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Universidad de Salamanca (USAL) |
| Repositorio: | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| OAI Identifier: | oai:gredos.usal.es:10366/166721 |
| Acceso en línea: | http://hdl.handle.net/10366/166721 https://doi.org/10.1186/s12934-021-01616-0 |
| Access Level: | acceso abierto |
| Palabra clave: | Fungal cell wall Cell wall synthases Glucanasas Polisacáridos Glucanos 2414 Microbiología 2407 Biología Celular 2414.10 Micología (Levaduras) 2415.01 Biología Molecular de Microorganismos 2302 Bioquímica |
| Sumario: | [EN]The fungal cell wall is an essential and robust external structure that protects the cell from the environment. It is mainly composed of polysaccharides with different functions, some of which are necessary for cell integrity. Thus, the process of fractionation and analysis of cell wall polysaccharides is useful for studying the function and relevance of each polysaccharide, as well as for developing a variety of practical and commercial applications. This method can be used to study the mechanisms that regulate cell morphogenesis and integrity, giving rise to information that could be applied in the design of new antifungal drugs. Nonetheless, for this method to be reliable, the availability of trustworthy commercial recombinant cell wall degrading enzymes with non-contaminating activities is vital. |
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