Cell Cycle Phase-Specific Surface Expression of Nerve Growth Factor Receptors TrkA and p75NTR

[EN]Expression of the nerve growth factor (NGF) receptors TrkA and p75NTR was found to vary at the surface of PC12 cells in a cell cycle phase-specific manner. This was evidenced by using flow cytometric and microscopic analysis of cell populations labeled with antibodies to the extracellular domain...

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Autores: Urdiales, José Luis, Becker, Elena, Andrieu, Muriel, Thomas, Annie, Jullien, Jérôme, van Grunsven, Leo A., Menut, Sophie, Evan, Gerard I., Martı́n-Zanca, Dionisio, Rudkin, Brian B.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:1998
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/146279
Acceso en línea:http://hdl.handle.net/10366/146279
Access Level:acceso abierto
Palabra clave:Signaling
PC12 cells
TrkA antibodies
p75NTR antibodies
Flow cytometry
Cell cycle
Neurotrophin
Receptor
Cell Cycle
Receptor, trkA
2490 Neurociencias
ciclo celular
receptor trkA
id ES_fb9da1c91d52bc7fa1bbdea1886434c3
oai_identifier_str oai:gredos.usal.es:10366/146279
network_acronym_str ES
network_name_str España
repository_id_str
spelling Cell Cycle Phase-Specific Surface Expression of Nerve Growth Factor Receptors TrkA and p75NTRUrdiales, José LuisBecker, ElenaAndrieu, MurielThomas, AnnieJullien, Jérômevan Grunsven, Leo A.Menut, SophieEvan, Gerard I.Martı́n-Zanca, DionisioRudkin, Brian B.SignalingPC12 cellsTrkA antibodiesp75NTR antibodiesFlow cytometryCell cycleFlow cytometryCell cycleNeurotrophinReceptorCell CycleReceptor, trkA2490 Neurocienciasciclo celularreceptor trkA[EN]Expression of the nerve growth factor (NGF) receptors TrkA and p75NTR was found to vary at the surface of PC12 cells in a cell cycle phase-specific manner. This was evidenced by using flow cytometric and microscopic analysis of cell populations labeled with antibodies to the extracellular domains of both receptors. Differential expression of these receptors also was evidenced by biotinylation of surface proteins and Western analysis, using antibodies specific for the extracellular domains of TrkA and p75NTR. TrkA is expressed most strongly at the cell surface in M and early G1 phases, whereas p75NTR is expressed mainly in late G1, S, and G2 phases. This expression reflects the molecular and cellular responses to NGF in specific phases of the cell cycle; in the G1 phase NGF elicits both the anti-mitogenic effect, i.e., inhibition of the G1 to S transition, and the differentiation response whereas a survival effect is provoked elsewhere in the cell cycle. A model is proposed relating these responses to the surface expression of the two receptors. These observations open the way for novel approaches to the investigation of the mechanism of NGF signal transduction.Journal of Neuroscience202120211998info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/146279reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1462792026-06-07T06:28:51Z
dc.title.none.fl_str_mv Cell Cycle Phase-Specific Surface Expression of Nerve Growth Factor Receptors TrkA and p75NTR
title Cell Cycle Phase-Specific Surface Expression of Nerve Growth Factor Receptors TrkA and p75NTR
spellingShingle Cell Cycle Phase-Specific Surface Expression of Nerve Growth Factor Receptors TrkA and p75NTR
Urdiales, José Luis
Signaling
PC12 cells
TrkA antibodies
p75NTR antibodies
Flow cytometry
Cell cycle
Flow cytometry
Cell cycle
Neurotrophin
Receptor
Cell Cycle
Receptor, trkA
2490 Neurociencias
ciclo celular
receptor trkA
title_short Cell Cycle Phase-Specific Surface Expression of Nerve Growth Factor Receptors TrkA and p75NTR
title_full Cell Cycle Phase-Specific Surface Expression of Nerve Growth Factor Receptors TrkA and p75NTR
title_fullStr Cell Cycle Phase-Specific Surface Expression of Nerve Growth Factor Receptors TrkA and p75NTR
title_full_unstemmed Cell Cycle Phase-Specific Surface Expression of Nerve Growth Factor Receptors TrkA and p75NTR
title_sort Cell Cycle Phase-Specific Surface Expression of Nerve Growth Factor Receptors TrkA and p75NTR
dc.creator.none.fl_str_mv Urdiales, José Luis
Becker, Elena
Andrieu, Muriel
Thomas, Annie
Jullien, Jérôme
van Grunsven, Leo A.
Menut, Sophie
Evan, Gerard I.
Martı́n-Zanca, Dionisio
Rudkin, Brian B.
author Urdiales, José Luis
author_facet Urdiales, José Luis
Becker, Elena
Andrieu, Muriel
Thomas, Annie
Jullien, Jérôme
van Grunsven, Leo A.
Menut, Sophie
Evan, Gerard I.
Martı́n-Zanca, Dionisio
Rudkin, Brian B.
author_role author
author2 Becker, Elena
Andrieu, Muriel
Thomas, Annie
Jullien, Jérôme
van Grunsven, Leo A.
Menut, Sophie
Evan, Gerard I.
Martı́n-Zanca, Dionisio
Rudkin, Brian B.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Signaling
PC12 cells
TrkA antibodies
p75NTR antibodies
Flow cytometry

Cell cycle
Flow cytometry
Cell cycle
Neurotrophin
Receptor
Cell Cycle
Receptor, trkA
2490 Neurociencias
ciclo celular
receptor trkA
topic Signaling
PC12 cells
TrkA antibodies
p75NTR antibodies
Flow cytometry
Cell cycle
Flow cytometry
Cell cycle
Neurotrophin
Receptor
Cell Cycle
Receptor, trkA
2490 Neurociencias
ciclo celular
receptor trkA
description [EN]Expression of the nerve growth factor (NGF) receptors TrkA and p75NTR was found to vary at the surface of PC12 cells in a cell cycle phase-specific manner. This was evidenced by using flow cytometric and microscopic analysis of cell populations labeled with antibodies to the extracellular domains of both receptors. Differential expression of these receptors also was evidenced by biotinylation of surface proteins and Western analysis, using antibodies specific for the extracellular domains of TrkA and p75NTR. TrkA is expressed most strongly at the cell surface in M and early G1 phases, whereas p75NTR is expressed mainly in late G1, S, and G2 phases. This expression reflects the molecular and cellular responses to NGF in specific phases of the cell cycle; in the G1 phase NGF elicits both the anti-mitogenic effect, i.e., inhibition of the G1 to S transition, and the differentiation response whereas a survival effect is provoked elsewhere in the cell cycle. A model is proposed relating these responses to the surface expression of the two receptors. These observations open the way for novel approaches to the investigation of the mechanism of NGF signal transduction.
publishDate 1998
dc.date.none.fl_str_mv 1998
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/146279
url http://hdl.handle.net/10366/146279
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Journal of Neuroscience
publisher.none.fl_str_mv Journal of Neuroscience
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869425345567916032
score 15,300719

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