Preclinical immunomodulation by the probiotic Bifidobacterium breve M-16V in early life

This study aimed to investigate the effect of supplementation with the probiotic Bifidobacterium breve M-16V on the maturation of the intestinal and circulating immune system during suckling. In order to achieve this purpose, neonatal Lewis rats were supplemented with the probiotic strain from the 6...

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Detalles Bibliográficos
Autores: Rigo Adrover, Maria del Mar, Franch i Masferrer, Àngels, Castell, Margarida, Pérez-Cano, Francisco J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/108362
Acceso en línea:https://hdl.handle.net/2445/108362
Access Level:acceso abierto
Palabra clave:Suplements nutritius
Probiòtics
Sistema immunològic
Rates (Animals de laboratori)
Dietary supplements
Probiotics
Immune system
Rats as laboratory animals
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spelling Preclinical immunomodulation by the probiotic Bifidobacterium breve M-16V in early lifeRigo Adrover, Maria del MarFranch i Masferrer, ÀngelsCastell, MargaridaPérez-Cano, Francisco J.Suplements nutritiusProbiòticsSistema immunològicRates (Animals de laboratori)Dietary supplementsProbioticsImmune systemRats as laboratory animalsThis study aimed to investigate the effect of supplementation with the probiotic Bifidobacterium breve M-16V on the maturation of the intestinal and circulating immune system during suckling. In order to achieve this purpose, neonatal Lewis rats were supplemented with the probiotic strain from the 6th to the 18th day of life. The animals were weighed during the study, and faecal samples were obtained and evaluated daily. On day 19, rats were euthanized and intestinal wash samples, mesenteric lymph node (MLN) cells, splenocytes and intraepithelial lymphocytes (IEL) were obtained. The probiotic supplementation in early life did not modify the growth curve and did not enhance the systemic immune maturation. However, it increased the proportion of cells bearing TLR4 in the MLN and IEL, and enhanced the percentage of the integrin αEβ7+ and CD62L+ cells in the MLN and that of the integrin αEβ7+ cells in the IEL, suggesting an enhancement of the homing process of naïve T lymphocytes to the MLN, and the retention of activated lymphocytes in the intraepithelial compartment. Interestingly, B. breve M-16V enhanced the intestinal IgA synthesis. In conclusion, supplementation with the probiotic strain B. breve M-16V during suckling improves the development of mucosal immunity in early lifePublic Library of Science (PLoS)2017201720162017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/108362Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1371/journal.pone.0166082PLoS One, 2016, vol. 11, num. 11, p. e0166082https://doi.org/10.1371/journal.pone.0166082cc-by (c) Rigo-Adrover, Mar et al., 2016http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1083622026-05-29T05:05:01Z
dc.title.none.fl_str_mv Preclinical immunomodulation by the probiotic Bifidobacterium breve M-16V in early life
title Preclinical immunomodulation by the probiotic Bifidobacterium breve M-16V in early life
spellingShingle Preclinical immunomodulation by the probiotic Bifidobacterium breve M-16V in early life
Rigo Adrover, Maria del Mar
Suplements nutritius
Probiòtics
Sistema immunològic
Rates (Animals de laboratori)
Dietary supplements
Probiotics
Immune system
Rats as laboratory animals
title_short Preclinical immunomodulation by the probiotic Bifidobacterium breve M-16V in early life
title_full Preclinical immunomodulation by the probiotic Bifidobacterium breve M-16V in early life
title_fullStr Preclinical immunomodulation by the probiotic Bifidobacterium breve M-16V in early life
title_full_unstemmed Preclinical immunomodulation by the probiotic Bifidobacterium breve M-16V in early life
title_sort Preclinical immunomodulation by the probiotic Bifidobacterium breve M-16V in early life
dc.creator.none.fl_str_mv Rigo Adrover, Maria del Mar
Franch i Masferrer, Àngels
Castell, Margarida
Pérez-Cano, Francisco J.
author Rigo Adrover, Maria del Mar
author_facet Rigo Adrover, Maria del Mar
Franch i Masferrer, Àngels
Castell, Margarida
Pérez-Cano, Francisco J.
author_role author
author2 Franch i Masferrer, Àngels
Castell, Margarida
Pérez-Cano, Francisco J.
author2_role author
author
author
dc.subject.none.fl_str_mv Suplements nutritius
Probiòtics
Sistema immunològic
Rates (Animals de laboratori)
Dietary supplements
Probiotics
Immune system
Rats as laboratory animals
topic Suplements nutritius
Probiòtics
Sistema immunològic
Rates (Animals de laboratori)
Dietary supplements
Probiotics
Immune system
Rats as laboratory animals
description This study aimed to investigate the effect of supplementation with the probiotic Bifidobacterium breve M-16V on the maturation of the intestinal and circulating immune system during suckling. In order to achieve this purpose, neonatal Lewis rats were supplemented with the probiotic strain from the 6th to the 18th day of life. The animals were weighed during the study, and faecal samples were obtained and evaluated daily. On day 19, rats were euthanized and intestinal wash samples, mesenteric lymph node (MLN) cells, splenocytes and intraepithelial lymphocytes (IEL) were obtained. The probiotic supplementation in early life did not modify the growth curve and did not enhance the systemic immune maturation. However, it increased the proportion of cells bearing TLR4 in the MLN and IEL, and enhanced the percentage of the integrin αEβ7+ and CD62L+ cells in the MLN and that of the integrin αEβ7+ cells in the IEL, suggesting an enhancement of the homing process of naïve T lymphocytes to the MLN, and the retention of activated lymphocytes in the intraepithelial compartment. Interestingly, B. breve M-16V enhanced the intestinal IgA synthesis. In conclusion, supplementation with the probiotic strain B. breve M-16V during suckling improves the development of mucosal immunity in early life
publishDate 2016
dc.date.none.fl_str_mv 2016
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/108362
url https://hdl.handle.net/2445/108362
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0166082
PLoS One, 2016, vol. 11, num. 11, p. e0166082
https://doi.org/10.1371/journal.pone.0166082
dc.rights.none.fl_str_mv cc-by (c) Rigo-Adrover, Mar et al., 2016
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Rigo-Adrover, Mar et al., 2016
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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