SARS-CoV-2 Evolution and Spike-Specific CD4+ T-Cell Response in Persistent COVID-19 with Severe HIV Immune Suppression

Intra-host evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been reported in cases with persistent coronavirus disease 2019 (COVID-19). In this study, we describe a severely immunosuppressed individual with HIV-1/SARS-CoV-2 coinfection with a long-term course of SARS-CoV...

ver descrição completa

Detalhes bibliográficos
Autores: Álvarez, Hortensia, Ruiz-Mateos, Ezequiel, Juiz-González, Pedro Miguel, Vitallé, Joana, Vieitez, Irene, Vázquez-Friol, María del Carmen, Torres-Beceiro, Isabel, Pérez-Gómez, Alberto, Gallego-García, Pilar, Estévez-Gómez, Nuria, Chiara , Loretta de, Poveda, Eva, Posada, David, Llibre, Josep María
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/268921
Acesso em linha:http://hdl.handle.net/10261/268921
Access Level:acceso abierto
Palavra-chave:SARS-CoV-2
HIV
CD4+ T cell response
id ES_fb8879918942e16eea6849ff6782db5d
oai_identifier_str oai:digital.csic.es:10261/268921
network_acronym_str ES
network_name_str España
repository_id_str
spelling SARS-CoV-2 Evolution and Spike-Specific CD4+ T-Cell Response in Persistent COVID-19 with Severe HIV Immune SuppressionÁlvarez, HortensiaRuiz-Mateos, EzequielJuiz-González, Pedro MiguelVitallé, JoanaVieitez, IreneVázquez-Friol, María del CarmenTorres-Beceiro, IsabelPérez-Gómez, AlbertoGallego-García, PilarEstévez-Gómez, NuriaChiara , Loretta dePoveda, EvaPosada, DavidLlibre, Josep MaríaSARS-CoV-2HIVCD4+ T cell responseIntra-host evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been reported in cases with persistent coronavirus disease 2019 (COVID-19). In this study, we describe a severely immunosuppressed individual with HIV-1/SARS-CoV-2 coinfection with a long-term course of SARS-CoV-2 infection. A 28-year-old man was diagnosed with HIV-1 infection (CD4+ count: 3 cells/µL nd 563000 HIV-1 RNA copies/mL) and simultaneous Pneumocystis jirovecii pneumonia, disseminated Mycobacterium avium complex infection and SARS-CoV-2 infection. SARS-CoV-2 real-time reverse transcription polymerase chain reaction positivity from nasopharyngeal samples was prolonged for 15 weeks. SARS-CoV-2 was identified as variant Alpha (PANGO lineage B.1.1.7) with mutation S:E484K. Spike-specific T-cell response was similar to HIV-negative controls although enriched in IL-2, and showed disproportionately increased immunological exhaustion marker levels. Despite persistent SARS-CoV-2 infection, adaptive intra-host SARS-CoV-2 evolution, was not identified. Spike-specific T-cell response protected against a severe COVID-19 outcome and the increased immunological exhaustion marker levels might have favoured SARS-CoV-2 persistence.This work was partially funded by grant EPICOVIGAL from FONDO SUPERA-COVID19 from Banco Santander-CSIC-CRUE and grant CT850A-2 from ACIS SERGAS from the Consellería de Sanidade of Xunta de Galicia, Spain, to D.P.Peer reviewedMolecular Diversity Preservation InternationalBanco SantanderConsejo Superior de Investigaciones Científicas (España)Conferencia de Rectores de las Universidades EspañolasXunta de GaliciaÁlvarez, Hortensia [0000-0002-5002-1737]Ruiz-Mateos, Ezequiel [0000-0001-6747-7813]Vitallé, Joana [0000-0002-5292-1851]Vieitez, Irene [0000-0001-9241-0207]Pérez-Gómez, Alberto [0000-0002-3644-2914]Gallego-García, Pilar [0000-0001-8531-9851]Chiara , Loretta de [0000-0001-7700-6417]Poveda, Eva [0000-0003-4835-9875]Posada, David [0000-0003-1407-3406]Llibre, Josep María [0000-0002-7158-6753]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/268921reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/microorganisms10010143https://doi.org/10.3390/microorganisms10010143Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2689212026-05-22T06:33:51Z
dc.title.none.fl_str_mv SARS-CoV-2 Evolution and Spike-Specific CD4+ T-Cell Response in Persistent COVID-19 with Severe HIV Immune Suppression
title SARS-CoV-2 Evolution and Spike-Specific CD4+ T-Cell Response in Persistent COVID-19 with Severe HIV Immune Suppression
spellingShingle SARS-CoV-2 Evolution and Spike-Specific CD4+ T-Cell Response in Persistent COVID-19 with Severe HIV Immune Suppression
Álvarez, Hortensia
SARS-CoV-2
HIV
CD4+ T cell response
title_short SARS-CoV-2 Evolution and Spike-Specific CD4+ T-Cell Response in Persistent COVID-19 with Severe HIV Immune Suppression
title_full SARS-CoV-2 Evolution and Spike-Specific CD4+ T-Cell Response in Persistent COVID-19 with Severe HIV Immune Suppression
title_fullStr SARS-CoV-2 Evolution and Spike-Specific CD4+ T-Cell Response in Persistent COVID-19 with Severe HIV Immune Suppression
title_full_unstemmed SARS-CoV-2 Evolution and Spike-Specific CD4+ T-Cell Response in Persistent COVID-19 with Severe HIV Immune Suppression
title_sort SARS-CoV-2 Evolution and Spike-Specific CD4+ T-Cell Response in Persistent COVID-19 with Severe HIV Immune Suppression
dc.creator.none.fl_str_mv Álvarez, Hortensia
Ruiz-Mateos, Ezequiel
Juiz-González, Pedro Miguel
Vitallé, Joana
Vieitez, Irene
Vázquez-Friol, María del Carmen
Torres-Beceiro, Isabel
Pérez-Gómez, Alberto
Gallego-García, Pilar
Estévez-Gómez, Nuria
Chiara , Loretta de
Poveda, Eva
Posada, David
Llibre, Josep María
author Álvarez, Hortensia
author_facet Álvarez, Hortensia
Ruiz-Mateos, Ezequiel
Juiz-González, Pedro Miguel
Vitallé, Joana
Vieitez, Irene
Vázquez-Friol, María del Carmen
Torres-Beceiro, Isabel
Pérez-Gómez, Alberto
Gallego-García, Pilar
Estévez-Gómez, Nuria
Chiara , Loretta de
Poveda, Eva
Posada, David
Llibre, Josep María
author_role author
author2 Ruiz-Mateos, Ezequiel
Juiz-González, Pedro Miguel
Vitallé, Joana
Vieitez, Irene
Vázquez-Friol, María del Carmen
Torres-Beceiro, Isabel
Pérez-Gómez, Alberto
Gallego-García, Pilar
Estévez-Gómez, Nuria
Chiara , Loretta de
Poveda, Eva
Posada, David
Llibre, Josep María
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Banco Santander
Consejo Superior de Investigaciones Científicas (España)
Conferencia de Rectores de las Universidades Españolas
Xunta de Galicia
Álvarez, Hortensia [0000-0002-5002-1737]
Ruiz-Mateos, Ezequiel [0000-0001-6747-7813]
Vitallé, Joana [0000-0002-5292-1851]
Vieitez, Irene [0000-0001-9241-0207]
Pérez-Gómez, Alberto [0000-0002-3644-2914]
Gallego-García, Pilar [0000-0001-8531-9851]
Chiara , Loretta de [0000-0001-7700-6417]
Poveda, Eva [0000-0003-4835-9875]
Posada, David [0000-0003-1407-3406]
Llibre, Josep María [0000-0002-7158-6753]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv SARS-CoV-2
HIV
CD4+ T cell response
topic SARS-CoV-2
HIV
CD4+ T cell response
description Intra-host evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been reported in cases with persistent coronavirus disease 2019 (COVID-19). In this study, we describe a severely immunosuppressed individual with HIV-1/SARS-CoV-2 coinfection with a long-term course of SARS-CoV-2 infection. A 28-year-old man was diagnosed with HIV-1 infection (CD4+ count: 3 cells/µL nd 563000 HIV-1 RNA copies/mL) and simultaneous Pneumocystis jirovecii pneumonia, disseminated Mycobacterium avium complex infection and SARS-CoV-2 infection. SARS-CoV-2 real-time reverse transcription polymerase chain reaction positivity from nasopharyngeal samples was prolonged for 15 weeks. SARS-CoV-2 was identified as variant Alpha (PANGO lineage B.1.1.7) with mutation S:E484K. Spike-specific T-cell response was similar to HIV-negative controls although enriched in IL-2, and showed disproportionately increased immunological exhaustion marker levels. Despite persistent SARS-CoV-2 infection, adaptive intra-host SARS-CoV-2 evolution, was not identified. Spike-specific T-cell response protected against a severe COVID-19 outcome and the increased immunological exhaustion marker levels might have favoured SARS-CoV-2 persistence.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/268921
url http://hdl.handle.net/10261/268921
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/microorganisms10010143
https://doi.org/10.3390/microorganisms10010143
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869425337882902528
score 15,812455

Registos relacionados