RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi

Powdery mildew and rust fungi are major agricultural problems affecting many economically important crops and causing significant yield losses. These fungi are obligate biotrophic parasites that are completely dependent on their hosts for growth and reproduction. Biotrophy in these fungi is determin...

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Detalhes bibliográficos
Autores: Padilla-Roji, Isabel, Ruiz-Jiménez, Laura, Bakhat, Nisrine, Vielba-Fernández, Alejandra, Pérez-García, Alejandro, Fernández-Ortuño, Dolores
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/371771
Acesso em linha:http://hdl.handle.net/10261/371771
https://api.elsevier.com/content/abstract/scopus_id/85160379520
Access Level:acceso abierto
Palavra-chave:ATM-HIGS
HIGS
RNA interference
SIGS
VIGS
dsRNA
powdery mildew
rust
transgenic plants
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spelling RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic FungiPadilla-Roji, IsabelRuiz-Jiménez, LauraBakhat, NisrineVielba-Fernández, AlejandraPérez-García, AlejandroFernández-Ortuño, DoloresATM-HIGSHIGSRNA interferenceSIGSVIGSdsRNApowdery mildewrusttransgenic plantsPowdery mildew and rust fungi are major agricultural problems affecting many economically important crops and causing significant yield losses. These fungi are obligate biotrophic parasites that are completely dependent on their hosts for growth and reproduction. Biotrophy in these fungi is determined by the presence of haustoria, specialized fungal cells that are responsible for nutrient uptake and molecular dialogue with the host, a fact that undoubtedly complicates their study under laboratory conditions, especially in terms of genetic manipulation. RNA interference (RNAi) is the biological process of suppressing the expression of a target gene through double-stranded RNA that induces mRNA degradation. RNAi technology has revolutionized the study of these obligate biotrophic fungi by enabling the analysis of gene function in these fungal. More importantly, RNAi technology has opened new perspectives for the management of powdery mildew and rust diseases, first through the stable expression of RNAi constructs in transgenic plants and, more recently, through the non-transgenic approach called spray-induced gene silencing (SIGS). In this review, the impact of RNAi technology on the research and management of powdery mildew and rust fungi will be addressed.This publication is part of the R+D+i projects PID2019-107464RB-C21 and PDC2021- 121373-C21, funded by MICIN/AEI//10.13039/501100011033 and by the “European Union NextGen erationEU/PRTR”. I.P.-R. was supported by a Ph.D. fellowship also from AEI, grant number PRE2020-093156.Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)European Commission0000-0003-2147-358X0009-0009-8304-84240000-0002-1065-03600000-0002-3134-3095Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/371771https://api.elsevier.com/content/abstract/scopus_id/85160379520reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107464RB-C21info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PDC2021-121373-C21International journal of molecular scienceshttps://doi.org/10.3390/ijms24109082Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3717712026-05-22T06:33:51Z
dc.title.none.fl_str_mv RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi
title RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi
spellingShingle RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi
Padilla-Roji, Isabel
ATM-HIGS
HIGS
RNA interference
SIGS
VIGS
dsRNA
powdery mildew
rust
transgenic plants
title_short RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi
title_full RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi
title_fullStr RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi
title_full_unstemmed RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi
title_sort RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi
dc.creator.none.fl_str_mv Padilla-Roji, Isabel
Ruiz-Jiménez, Laura
Bakhat, Nisrine
Vielba-Fernández, Alejandra
Pérez-García, Alejandro
Fernández-Ortuño, Dolores
author Padilla-Roji, Isabel
author_facet Padilla-Roji, Isabel
Ruiz-Jiménez, Laura
Bakhat, Nisrine
Vielba-Fernández, Alejandra
Pérez-García, Alejandro
Fernández-Ortuño, Dolores
author_role author
author2 Ruiz-Jiménez, Laura
Bakhat, Nisrine
Vielba-Fernández, Alejandra
Pérez-García, Alejandro
Fernández-Ortuño, Dolores
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
European Commission
0000-0003-2147-358X
0009-0009-8304-8424
0000-0002-1065-0360
0000-0002-3134-3095
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv ATM-HIGS
HIGS
RNA interference
SIGS
VIGS
dsRNA
powdery mildew
rust
transgenic plants
topic ATM-HIGS
HIGS
RNA interference
SIGS
VIGS
dsRNA
powdery mildew
rust
transgenic plants
description Powdery mildew and rust fungi are major agricultural problems affecting many economically important crops and causing significant yield losses. These fungi are obligate biotrophic parasites that are completely dependent on their hosts for growth and reproduction. Biotrophy in these fungi is determined by the presence of haustoria, specialized fungal cells that are responsible for nutrient uptake and molecular dialogue with the host, a fact that undoubtedly complicates their study under laboratory conditions, especially in terms of genetic manipulation. RNA interference (RNAi) is the biological process of suppressing the expression of a target gene through double-stranded RNA that induces mRNA degradation. RNAi technology has revolutionized the study of these obligate biotrophic fungi by enabling the analysis of gene function in these fungal. More importantly, RNAi technology has opened new perspectives for the management of powdery mildew and rust diseases, first through the stable expression of RNAi constructs in transgenic plants and, more recently, through the non-transgenic approach called spray-induced gene silencing (SIGS). In this review, the impact of RNAi technology on the research and management of powdery mildew and rust fungi will be addressed.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/371771
https://api.elsevier.com/content/abstract/scopus_id/85160379520
url http://hdl.handle.net/10261/371771
https://api.elsevier.com/content/abstract/scopus_id/85160379520
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107464RB-C21
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PDC2021-121373-C21
International journal of molecular sciences
https://doi.org/10.3390/ijms24109082
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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