Differential expression of five prosomatostatin genes in the central nervous system of the catshark Scyliorhinus canicula

Five prosomatostatin genes (PSST1, PSST2, PSST3, PSST5, and PSST6) have been recently identified in elasmobranchs (Tostivint et al., General and Comparative Endocrinology, 2019, 279, 139–147). In order to gain insight into the contribution of each somatostatin to specific nervous systems circuits an...

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Autores: Sobrido Cameán, Daniel, Tostivint, Hervé, Mazan, Sylvie, Rodicio Rodicio, María Celina, Rodríguez-Moldes Rey, María Isabel, Candal Suárez, Eva María, Anadón Álvarez, Ramón, Barreiro Iglesias, Antón
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglés
OAI Identifier:oai:minerva.usc.gal:10347/44200
Acceso en línea:https://hdl.handle.net/10347/44200
Access Level:acceso abierto
Palabra clave:Brain
Differential gene expression
Elasmobranchs
Shark
Somatostatin precursors
Spinal cord
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spelling Differential expression of five prosomatostatin genes in the central nervous system of the catshark Scyliorhinus caniculaSobrido Cameán, DanielTostivint, HervéMazan, SylvieRodicio Rodicio, María CelinaRodríguez-Moldes Rey, María IsabelCandal Suárez, Eva MaríaAnadón Álvarez, RamónBarreiro Iglesias, AntónBrainDifferential gene expressionElasmobranchsSharkSomatostatin precursorsSpinal cordFive prosomatostatin genes (PSST1, PSST2, PSST3, PSST5, and PSST6) have been recently identified in elasmobranchs (Tostivint et al., General and Comparative Endocrinology, 2019, 279, 139–147). In order to gain insight into the contribution of each somatostatin to specific nervous systems circuits and behaviors in this important jawed vertebrate group, we studied the distribution of neurons expressing PSST mRNAs in the central nervous system (CNS) of Scyliorhinus canicula using in situ hybridization. Additionally, we combined in situ hybridization with tyrosine hydroxylase (TH) immunochemistry for better characterization of PSST1 and PSST6 expressing populations. We observed differential expression of PSST1 and PSST6, which are the most widely expressed PSST transcripts, in cell populations of many CNS regions, including the pallium, subpallium, hypothalamus, diencephalon, optic tectum, midbrain tegmentum, and rhombencephalon. Interestingly, numerous small pallial neurons express PSST1 and PSST6, although in different populations judging from the colocalization of TH immunoreactivity and PSST6 expression but not with PSST1. We observed expression of PSST1 in cerebrospinal fluid-contacting (CSF-c) neurons of the hypothalamic paraventricular organ and the central canal of the spinal cord. Unlike PSST1 and PSST6, PSST2, and PSST3 are only expressed in cells of the hypothalamus and in some hindbrain lateral reticular neurons, and PSST5 in cells of the region of the entopeduncular nucleus. Comparative data of brain expression of PSST genes indicate that the somatostatinergic system of sharks is the most complex reported in any fish.WileyUniversidade de Santiago de Compostela. Departamento de Bioloxía Funcional20202020-03-0520202020-03-05journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10347/44200reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 BFU2017-87079-P EL GABA PROMUEVE LA SUPERVIVENCIA NEURONAL Y LA REGENERACION AXONAL TRAS UNA LESION MEDULAR INHIBIENDO LA VIA DE NOTCHAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 BFU2017-89861-P DESCODIFICACION DE LAS BASES MOLECULARES DE LA NEUROGENESIS EN EL ADULTO MEDIANTE EL ANALISIS DE LA DINAMICA TRANSCRIPCIONAL DE NICHOS NEUROGENICOS DE LARGA DURACIONopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/442002026-06-15T12:47:27Z
dc.title.none.fl_str_mv Differential expression of five prosomatostatin genes in the central nervous system of the catshark Scyliorhinus canicula
title Differential expression of five prosomatostatin genes in the central nervous system of the catshark Scyliorhinus canicula
spellingShingle Differential expression of five prosomatostatin genes in the central nervous system of the catshark Scyliorhinus canicula
Sobrido Cameán, Daniel
Brain
Differential gene expression
Elasmobranchs
Shark
Somatostatin precursors
Spinal cord
title_short Differential expression of five prosomatostatin genes in the central nervous system of the catshark Scyliorhinus canicula
title_full Differential expression of five prosomatostatin genes in the central nervous system of the catshark Scyliorhinus canicula
title_fullStr Differential expression of five prosomatostatin genes in the central nervous system of the catshark Scyliorhinus canicula
title_full_unstemmed Differential expression of five prosomatostatin genes in the central nervous system of the catshark Scyliorhinus canicula
title_sort Differential expression of five prosomatostatin genes in the central nervous system of the catshark Scyliorhinus canicula
dc.creator.none.fl_str_mv Sobrido Cameán, Daniel
Tostivint, Hervé
Mazan, Sylvie
Rodicio Rodicio, María Celina
Rodríguez-Moldes Rey, María Isabel
Candal Suárez, Eva María
Anadón Álvarez, Ramón
Barreiro Iglesias, Antón
author Sobrido Cameán, Daniel
author_facet Sobrido Cameán, Daniel
Tostivint, Hervé
Mazan, Sylvie
Rodicio Rodicio, María Celina
Rodríguez-Moldes Rey, María Isabel
Candal Suárez, Eva María
Anadón Álvarez, Ramón
Barreiro Iglesias, Antón
author_role author
author2 Tostivint, Hervé
Mazan, Sylvie
Rodicio Rodicio, María Celina
Rodríguez-Moldes Rey, María Isabel
Candal Suárez, Eva María
Anadón Álvarez, Ramón
Barreiro Iglesias, Antón
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de Santiago de Compostela. Departamento de Bioloxía Funcional
dc.subject.none.fl_str_mv Brain
Differential gene expression
Elasmobranchs
Shark
Somatostatin precursors
Spinal cord
topic Brain
Differential gene expression
Elasmobranchs
Shark
Somatostatin precursors
Spinal cord
description Five prosomatostatin genes (PSST1, PSST2, PSST3, PSST5, and PSST6) have been recently identified in elasmobranchs (Tostivint et al., General and Comparative Endocrinology, 2019, 279, 139–147). In order to gain insight into the contribution of each somatostatin to specific nervous systems circuits and behaviors in this important jawed vertebrate group, we studied the distribution of neurons expressing PSST mRNAs in the central nervous system (CNS) of Scyliorhinus canicula using in situ hybridization. Additionally, we combined in situ hybridization with tyrosine hydroxylase (TH) immunochemistry for better characterization of PSST1 and PSST6 expressing populations. We observed differential expression of PSST1 and PSST6, which are the most widely expressed PSST transcripts, in cell populations of many CNS regions, including the pallium, subpallium, hypothalamus, diencephalon, optic tectum, midbrain tegmentum, and rhombencephalon. Interestingly, numerous small pallial neurons express PSST1 and PSST6, although in different populations judging from the colocalization of TH immunoreactivity and PSST6 expression but not with PSST1. We observed expression of PSST1 in cerebrospinal fluid-contacting (CSF-c) neurons of the hypothalamic paraventricular organ and the central canal of the spinal cord. Unlike PSST1 and PSST6, PSST2, and PSST3 are only expressed in cells of the hypothalamus and in some hindbrain lateral reticular neurons, and PSST5 in cells of the region of the entopeduncular nucleus. Comparative data of brain expression of PSST genes indicate that the somatostatinergic system of sharks is the most complex reported in any fish.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-03-05
2020
2020-03-05
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10347/44200
url https://hdl.handle.net/10347/44200
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 BFU2017-87079-P EL GABA PROMUEVE LA SUPERVIVENCIA NEURONAL Y LA REGENERACION AXONAL TRAS UNA LESION MEDULAR INHIBIENDO LA VIA DE NOTCH
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 BFU2017-89861-P DESCODIFICACION DE LAS BASES MOLECULARES DE LA NEUROGENESIS EN EL ADULTO MEDIANTE EL ANALISIS DE LA DINAMICA TRANSCRIPCIONAL DE NICHOS NEUROGENICOS DE LARGA DURACION
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
collection Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
repository.name.fl_str_mv
repository.mail.fl_str_mv
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