Impact of cryopreservation on motile subpopulations and tyrosine-phosphorylated regions of ram spermatozoa during capacitating conditions

This article belongs to the Special Issue Factors Affecting In Vitro Assessment of Sperm Quality.

Detalles Bibliográficos
Autores: Peris-Frau, Patricia, Sánchez-Ajofrín, Irene, Martín-Maestro, Alicia, Maside, Carolina, Medina-Chávez, Daniela-Alejandra, García-Álvarez, Olga, Fernández-Santos, M. Rocío, Montoro, Vidal, Garde, José Julián, Ramón, Manuel, Soler, Ana J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/265038
Acceso en línea:http://hdl.handle.net/10261/265038
https://doi.org/10.3390/biology10111213
Access Level:acceso abierto
Palabra clave:Computer-assisted sperm analysis
Tyrosine phosphorylation
Cytometry
Sperm cryopreservation
Subpopulations
Sperm capacitation
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spelling Impact of cryopreservation on motile subpopulations and tyrosine-phosphorylated regions of ram spermatozoa during capacitating conditionsPeris-Frau, PatriciaSánchez-Ajofrín, IreneMartín-Maestro, AliciaMaside, CarolinaMedina-Chávez, Daniela-AlejandraGarcía-Álvarez, OlgaFernández-Santos, M. RocíoMontoro, VidalGarde, José JuliánRamón, ManuelSoler, Ana J.Computer-assisted sperm analysisTyrosine phosphorylationCytometrySperm cryopreservationSubpopulationsSperm capacitationThis article belongs to the Special Issue Factors Affecting In Vitro Assessment of Sperm Quality.The heterogeneous nature of ejaculates highlights the relevance of studying the behavior of different sperm subpopulations. Changes in sperm motility and the increase in tyrosine phosphorylation are key events that usually occur during capacitation and can be modified by the cryopreservation process. However, the relationship between both events remains poorly defined throughout capacitation in the different sperm subpopulations. Fresh and frozen-thawed spermatozoa were incubated in capacitating (CAP) and non-capacitating (NC) media up to 240 min. Sperm kinematics, tyrosine phosphorylation and mitochondrial activity were measured by the CASA system and imaging flow cytometry. Four motile sperm subpopulations (SP) were identified in fresh and frozen-thawed ram semen after the cluster analysis. Incubation under CAP conditions over time led to greater changes in the percentage of spermatozoa included in each subpopulation compared to NC conditions, being different between fresh and frozen-thawed spermatozoa. The SP1, characterized by slow spermatozoa, progressively increased after 15 min in frozen-thawed samples incubated in both media but not in fresh ones. The SP4, characterized by fast and non-linear spermatozoa, showed a marked increase during CAP, but not under NC conditions, occurring more rapidly in frozen-thawed spermatozoa. This subpopulation (SP4) was also the only one positively and strongly correlated with mitochondrial activity and all phosphorylated sperm regions during capacitation, either in fresh or frozen-thawed samples. Our results indicated that in vitro capacitation induced significant changes in the distribution of motile sperm subpopulations, being affected by cryopreservation. Notwithstanding, the subpopulation which probably represents hyperactivated-like spermatozoa (SP4) also increased in frozen-thawed samples, occurring faster and simultaneously to the increment of mitochondrial activity and tyrosine phosphorylation of different sperm regions.This research was supported by the Spanish Ministry of Economy and Competitiveness (AGL2013-48421-R and AGL2017-89017-R). A.M.M. was supported by a Ministry of Economy and Competitiveness scholarship and P.P.-F. was supported by a University of Castilla-La Mancha scholarship [2015/4062].Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Economía y Competitividad (España)Agencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Universidad de Castilla La ManchaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/265038https://doi.org/10.3390/biology10111213reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//AGL2013-48421-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-89017-Rhttps://doi.org/10.3390/biology10111213Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2650382026-05-22T06:33:51Z
dc.title.none.fl_str_mv Impact of cryopreservation on motile subpopulations and tyrosine-phosphorylated regions of ram spermatozoa during capacitating conditions
title Impact of cryopreservation on motile subpopulations and tyrosine-phosphorylated regions of ram spermatozoa during capacitating conditions
spellingShingle Impact of cryopreservation on motile subpopulations and tyrosine-phosphorylated regions of ram spermatozoa during capacitating conditions
Peris-Frau, Patricia
Computer-assisted sperm analysis
Tyrosine phosphorylation
Cytometry
Sperm cryopreservation
Subpopulations
Sperm capacitation
title_short Impact of cryopreservation on motile subpopulations and tyrosine-phosphorylated regions of ram spermatozoa during capacitating conditions
title_full Impact of cryopreservation on motile subpopulations and tyrosine-phosphorylated regions of ram spermatozoa during capacitating conditions
title_fullStr Impact of cryopreservation on motile subpopulations and tyrosine-phosphorylated regions of ram spermatozoa during capacitating conditions
title_full_unstemmed Impact of cryopreservation on motile subpopulations and tyrosine-phosphorylated regions of ram spermatozoa during capacitating conditions
title_sort Impact of cryopreservation on motile subpopulations and tyrosine-phosphorylated regions of ram spermatozoa during capacitating conditions
dc.creator.none.fl_str_mv Peris-Frau, Patricia
Sánchez-Ajofrín, Irene
Martín-Maestro, Alicia
Maside, Carolina
Medina-Chávez, Daniela-Alejandra
García-Álvarez, Olga
Fernández-Santos, M. Rocío
Montoro, Vidal
Garde, José Julián
Ramón, Manuel
Soler, Ana J.
author Peris-Frau, Patricia
author_facet Peris-Frau, Patricia
Sánchez-Ajofrín, Irene
Martín-Maestro, Alicia
Maside, Carolina
Medina-Chávez, Daniela-Alejandra
García-Álvarez, Olga
Fernández-Santos, M. Rocío
Montoro, Vidal
Garde, José Julián
Ramón, Manuel
Soler, Ana J.
author_role author
author2 Sánchez-Ajofrín, Irene
Martín-Maestro, Alicia
Maside, Carolina
Medina-Chávez, Daniela-Alejandra
García-Álvarez, Olga
Fernández-Santos, M. Rocío
Montoro, Vidal
Garde, José Julián
Ramón, Manuel
Soler, Ana J.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Universidad de Castilla La Mancha
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Computer-assisted sperm analysis
Tyrosine phosphorylation
Cytometry
Sperm cryopreservation
Subpopulations
Sperm capacitation
topic Computer-assisted sperm analysis
Tyrosine phosphorylation
Cytometry
Sperm cryopreservation
Subpopulations
Sperm capacitation
description This article belongs to the Special Issue Factors Affecting In Vitro Assessment of Sperm Quality.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/265038
https://doi.org/10.3390/biology10111213
url http://hdl.handle.net/10261/265038
https://doi.org/10.3390/biology10111213
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO//AGL2013-48421-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-89017-R
https://doi.org/10.3390/biology10111213
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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