Maintenance of determination by cells of imaginal discs of Drosophila after dissociation and culture in vivo

Wild type cells of imaginal wing discs or embryos were dissociated and mixed in different proportions with cells of genetically marked wing and/or leg discs. These latter were X-irradiated to such an extent that their rate of proliferation was drastically lowered. They served as a ldquofeeding layer...

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Detalles Bibliográficos
Autores: García-Bellido, Antonio, Nöthiger, Rolf
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:1976
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/47523
Acceso en línea:http://hdl.handle.net/10261/47523
Access Level:acceso abierto
Palabra clave:Drosophila
Imaginal
Disc
Wing
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spelling Maintenance of determination by cells of imaginal discs of Drosophila after dissociation and culture in vivoGarcía-Bellido, AntonioNöthiger, RolfDrosophilaImaginalDiscWingWild type cells of imaginal wing discs or embryos were dissociated and mixed in different proportions with cells of genetically marked wing and/or leg discs. These latter were X-irradiated to such an extent that their rate of proliferation was drastically lowered. They served as a ldquofeeding layerrdquo in which the interspersed wild type cells could be cultured. The reaggregates were allowed to grow in vivo, and fragments of them were tested for recovery of imaginal structures formed by wild-type cells. The experimental conditions for maximal dilution and maximal recovery of wildtype cells were first analysed. Under these conditions the progeny of cells deriving from different fragments of mature wing discs are capable of forming large territories of cuticle. These consisted preferentially of structures located in the region from which their ancestral cells were derived. The proliferating cells remained confined to either the anterior or the posterior wing compartments, but were apparently able to transgress the dorsalventral compartment border. Cells with qualities for distinct imaginal discs and possibly regions could also be recovered from dissociated embryos of 7 h age. The efficiency with which imaginal structures could be recovered as well at the types or qualities of these structures did not depend on the histotype of the feeding layer.Peer reviewedSpringer NatureConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201220121976info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/47523reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1007/BF00848574Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/475232026-05-22T06:33:51Z
dc.title.none.fl_str_mv Maintenance of determination by cells of imaginal discs of Drosophila after dissociation and culture in vivo
title Maintenance of determination by cells of imaginal discs of Drosophila after dissociation and culture in vivo
spellingShingle Maintenance of determination by cells of imaginal discs of Drosophila after dissociation and culture in vivo
García-Bellido, Antonio
Drosophila
Imaginal
Disc
Wing
title_short Maintenance of determination by cells of imaginal discs of Drosophila after dissociation and culture in vivo
title_full Maintenance of determination by cells of imaginal discs of Drosophila after dissociation and culture in vivo
title_fullStr Maintenance of determination by cells of imaginal discs of Drosophila after dissociation and culture in vivo
title_full_unstemmed Maintenance of determination by cells of imaginal discs of Drosophila after dissociation and culture in vivo
title_sort Maintenance of determination by cells of imaginal discs of Drosophila after dissociation and culture in vivo
dc.creator.none.fl_str_mv García-Bellido, Antonio
Nöthiger, Rolf
author García-Bellido, Antonio
author_facet García-Bellido, Antonio
Nöthiger, Rolf
author_role author
author2 Nöthiger, Rolf
author2_role author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Drosophila
Imaginal
Disc
Wing
topic Drosophila
Imaginal
Disc
Wing
description Wild type cells of imaginal wing discs or embryos were dissociated and mixed in different proportions with cells of genetically marked wing and/or leg discs. These latter were X-irradiated to such an extent that their rate of proliferation was drastically lowered. They served as a ldquofeeding layerrdquo in which the interspersed wild type cells could be cultured. The reaggregates were allowed to grow in vivo, and fragments of them were tested for recovery of imaginal structures formed by wild-type cells. The experimental conditions for maximal dilution and maximal recovery of wildtype cells were first analysed. Under these conditions the progeny of cells deriving from different fragments of mature wing discs are capable of forming large territories of cuticle. These consisted preferentially of structures located in the region from which their ancestral cells were derived. The proliferating cells remained confined to either the anterior or the posterior wing compartments, but were apparently able to transgress the dorsalventral compartment border. Cells with qualities for distinct imaginal discs and possibly regions could also be recovered from dissociated embryos of 7 h age. The efficiency with which imaginal structures could be recovered as well at the types or qualities of these structures did not depend on the histotype of the feeding layer.
publishDate 1976
dc.date.none.fl_str_mv 1976
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/47523
url http://hdl.handle.net/10261/47523
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1007/BF00848574
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869425304656674816
score 15,198674

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