Optimization of hollow-fiber liquid phase microextraction for polychlorinated biphenyls in human breast milk

A reliable and sensitive analytical approach has been optimized for the extraction of seven polychlorinated biphenyls (PCBs) from human breast milk. Hollow fiber liquid phase microextraction (HF-LPME) was applied for the first time for the extraction and pre-concentration of the analytes. Analytes w...

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Detalles Bibliográficos
Autores: Villegas Álvarez, Maria Carmen, Callejón Leblic, María Belén, Rodríguez Moro, Gema, Gómez Ariza, José Luis, Arias Borrego, Ana, García Barrera, Tamara
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad de Huelva (UHU)
Repositorio:Arias Montano. Repositorio Institucional de la Universidad de Huelva
Idioma:inglés
OAI Identifier:oai:ariasmontano.uhu.es:10272/23241
Acceso en línea:https://hdl.handle.net/10272/23241
Access Level:acceso abierto
Palabra clave:Hollow Fiber Liquid Phase Microextraction
Breast milk
Polychlorinated Biphenyls
Gas chromatography
2301.03 Análisis Cromatográfico
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spelling Optimization of hollow-fiber liquid phase microextraction for polychlorinated biphenyls in human breast milkVillegas Álvarez, Maria CarmenCallejón Leblic, María BelénRodríguez Moro, GemaGómez Ariza, José LuisArias Borrego, AnaGarcía Barrera, TamaraHollow Fiber Liquid Phase MicroextractionBreast milkPolychlorinated BiphenylsGas chromatography2301.03 Análisis CromatográficoA reliable and sensitive analytical approach has been optimized for the extraction of seven polychlorinated biphenyls (PCBs) from human breast milk. Hollow fiber liquid phase microextraction (HF-LPME) was applied for the first time for the extraction and pre-concentration of the analytes. Analytes were separated by gas chromatography with electron capture detector (GC-μECD) for the sensitive detection and mass spectrometry for the unequivocal identification. A rotable central composite design (RCCD) was performed for the multivariate optimization of the method. The best results were obtained at 40 °C during 30 min and 600 rpm of stirring speed using a hollow fiber length of 5 cm and toluene as an extractant phase and salt addition was not required. The detection limits were in the range 7–14 ng L−1 for PCBs. The coefficients of determination of the calibration curves indicated good linearity (R2> 0.96) and the enrichment factors ranged from 74 to 143. This type of study is of great importance due to the deleterious effect that the presence of contaminants can produce in infants health related to the immature character of the defense system. Moreover, exclusive breastfeeding is recommended by neonatologists up to six months of life and as complementary food during the first two years.Elsevier B.V.20202020-01-0120202020-01-01journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10272/23241reponame:Arias Montano. Repositorio Institucional de la Universidad de Huelvainstname:Universidad de Huelva (UHU)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:ariasmontano.uhu.es:10272/232412026-06-02T14:58:11Z
dc.title.none.fl_str_mv Optimization of hollow-fiber liquid phase microextraction for polychlorinated biphenyls in human breast milk
title Optimization of hollow-fiber liquid phase microextraction for polychlorinated biphenyls in human breast milk
spellingShingle Optimization of hollow-fiber liquid phase microextraction for polychlorinated biphenyls in human breast milk
Villegas Álvarez, Maria Carmen
Hollow Fiber Liquid Phase Microextraction
Breast milk
Polychlorinated Biphenyls
Gas chromatography
2301.03 Análisis Cromatográfico
title_short Optimization of hollow-fiber liquid phase microextraction for polychlorinated biphenyls in human breast milk
title_full Optimization of hollow-fiber liquid phase microextraction for polychlorinated biphenyls in human breast milk
title_fullStr Optimization of hollow-fiber liquid phase microextraction for polychlorinated biphenyls in human breast milk
title_full_unstemmed Optimization of hollow-fiber liquid phase microextraction for polychlorinated biphenyls in human breast milk
title_sort Optimization of hollow-fiber liquid phase microextraction for polychlorinated biphenyls in human breast milk
dc.creator.none.fl_str_mv Villegas Álvarez, Maria Carmen
Callejón Leblic, María Belén
Rodríguez Moro, Gema
Gómez Ariza, José Luis
Arias Borrego, Ana
García Barrera, Tamara
author Villegas Álvarez, Maria Carmen
author_facet Villegas Álvarez, Maria Carmen
Callejón Leblic, María Belén
Rodríguez Moro, Gema
Gómez Ariza, José Luis
Arias Borrego, Ana
García Barrera, Tamara
author_role author
author2 Callejón Leblic, María Belén
Rodríguez Moro, Gema
Gómez Ariza, José Luis
Arias Borrego, Ana
García Barrera, Tamara
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Hollow Fiber Liquid Phase Microextraction
Breast milk
Polychlorinated Biphenyls
Gas chromatography
2301.03 Análisis Cromatográfico
topic Hollow Fiber Liquid Phase Microextraction
Breast milk
Polychlorinated Biphenyls
Gas chromatography
2301.03 Análisis Cromatográfico
description A reliable and sensitive analytical approach has been optimized for the extraction of seven polychlorinated biphenyls (PCBs) from human breast milk. Hollow fiber liquid phase microextraction (HF-LPME) was applied for the first time for the extraction and pre-concentration of the analytes. Analytes were separated by gas chromatography with electron capture detector (GC-μECD) for the sensitive detection and mass spectrometry for the unequivocal identification. A rotable central composite design (RCCD) was performed for the multivariate optimization of the method. The best results were obtained at 40 °C during 30 min and 600 rpm of stirring speed using a hollow fiber length of 5 cm and toluene as an extractant phase and salt addition was not required. The detection limits were in the range 7–14 ng L−1 for PCBs. The coefficients of determination of the calibration curves indicated good linearity (R2> 0.96) and the enrichment factors ranged from 74 to 143. This type of study is of great importance due to the deleterious effect that the presence of contaminants can produce in infants health related to the immature character of the defense system. Moreover, exclusive breastfeeding is recommended by neonatologists up to six months of life and as complementary food during the first two years.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01
2020
2020-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10272/23241
url https://hdl.handle.net/10272/23241
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Arias Montano. Repositorio Institucional de la Universidad de Huelva
instname:Universidad de Huelva (UHU)
instname_str Universidad de Huelva (UHU)
reponame_str Arias Montano. Repositorio Institucional de la Universidad de Huelva
collection Arias Montano. Repositorio Institucional de la Universidad de Huelva
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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