A Novel Active DNA Topoisomerase I in Leishmania donovani
[EN] A common feature shared by type I DNA topoisomerases is the presence of a “serine, lysine, X, X, tyrosine” motif as conventional enzyme active site. Preliminary data have shown that Leishmania donovani DNA topoisomerase I gene (LdTOP1A) lacked this conserved motif, giving rise to different theo...
| Autores: | , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2003 |
| País: | España |
| Institución: | Universidad de León |
| Repositorio: | BULERIA. Repositorio Institucional de la Universidad de León |
| OAI Identifier: | oai:buleria.unileon.es:10612/24313 |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0021925819307549?via%3Dihub https://hdl.handle.net/10612/24313 |
| Access Level: | acceso abierto |
| Palabra clave: | Toxicología DNA topoisomerase Leishmania donovani |
| Sumario: | [EN] A common feature shared by type I DNA topoisomerases is the presence of a “serine, lysine, X, X, tyrosine” motif as conventional enzyme active site. Preliminary data have shown that Leishmania donovani DNA topoisomerase I gene (LdTOP1A) lacked this conserved motif, giving rise to different theories about the reconstitution of an active DNA topoisomerase I in this parasite. We, herein, describe the molecular cloning of a new DNA topoisomerase I gene from L. donovani (LdTOP1B) containing the highly conserved serine, lysine, X, X, tyrosine motif. DNA topoisomerase I activity was detected only when both genes (LdTOP1A and LdTOP1B) were co-expressed in a yeast expression system, suggesting the existence of a dimeric DNA topoisomerase I in Leishmania parasites. |
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